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CpG-ODN with specific immunity stimulation function for PRRSV, and application of CpG-ODN

An immunostimulatory and specific technology, applied in the field of CpG-ODN, can solve the problem that there is no immunostimulatory unit, and achieve the effect of improving the induction of immune response, reducing the cost of breeding and reducing the incidence of disease.

Active Publication Date: 2021-04-09
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no research report on the immunostimulatory unit with immunostimulatory effect on PRRSV

Method used

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  • CpG-ODN with specific immunity stimulation function for PRRSV, and application of CpG-ODN
  • CpG-ODN with specific immunity stimulation function for PRRSV, and application of CpG-ODN
  • CpG-ODN with specific immunity stimulation function for PRRSV, and application of CpG-ODN

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Embodiment Construction

[0025] The technical solutions of the present invention will be further limited below in conjunction with specific embodiments, but the scope of protection is not limited to the descriptions made. The test methods used in the following examples are conventional methods unless otherwise specified, and the materials and reagents used are reagents or materials that can be directly obtained from commercial channels unless otherwise specified.

[0026] The CpG-ODN fragments are connected end to end to form a DNA sequence containing many -CG-dinucleotides as a CPG motif, which is called "Multi-CpG", that is, the immune activation unit.

[0027] Screening of Example Specific CpG-ODN Fragments

[0028] (1) Screening method: According to conventional techniques, CpG-ODN fragments were directional screened, and the results are shown in Table 1 below.

[0029] Table 1 CpG-ODN fragments screened

[0030]

[0031] The CpG-ODN fragments screened above are screened based on the research...

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Abstract

The invention relates to the technical field of bioengineering, in particular to CpG-ODN with a specific immunity stimulation function for PRRSV, and application of the CpG-ODN. A nucleotide sequence is disclosed by SEQ ID NO:1, 11 pieces of screened CpG-ODN fragments are used for randomly forming 10 groups of immune activation units, and in addition, the position information of each CpG-ODN fragment in each group of immune activation unit is different; and the 10 groups of immune activation units are inserted into a multi-copy cloning vector Puc19 deriving vector FVX through two digestion sites Xbal and EcoRI, a recombinant plasmid FVX-CpG is constructed through a gene synthesis means, and a preparation containing a recombinant plasmid FVX-CpG is prepared. The preparation has a low production cost, the defects of instability, sequence diversity, individual differences and the like of the CpG-ODN in the body are overcome, the in vivo natural immunologic function of a pig can be induced to be activated, the in vivo acquired immunity function of the pig for PRRSV can be activated, the in vivo immunoreaction induction function of the pig for the PRRSV can be improved, a PRRSV incidence rate is lowered, and a pig breeding cost is lowered.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a CpG-ODN with specific immunostimulatory effect on PRRSV and its application. Background technique [0002] Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is the causative agent of Porcine Reproductive and Respiratory Syndrome (PRRS). PRRSV was first discovered in the United States in 1987. In 1991, the Dutchman Wensvoot isolated the virus from sick piglets and sows for the first time, and then Germany, the United States, and the United Kingdom also isolated the virus. At present, the virus has spread throughout North America and Europe, and has spread globally, and epidemic infections have occurred successively in various places, causing serious economic losses to the pig industry. Although porcine reproductive and respiratory syndrome virus vaccines have been widely used, they are accompanied by side effects and have only partial immune protection, which m...

Claims

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Application Information

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IPC IPC(8): C12N15/117C12N15/85A61K39/39A61P31/14
CPCA61K39/39A61K2039/552A61K2039/55561A61P31/14C12N15/117C12N15/85C12N2310/17C12N2800/107
Inventor 冯璥李涛何文彬吕雪梅
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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