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Application of marinobacterium in preparation of polyhydroxyalkanoate

A technology of polyhydroxyalkanoate and sea bacteria, which is applied in the field of microbiology and bioengineering, and can solve the problems of restricting large-scale production and application and high production cost

Pending Publication Date: 2021-04-09
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although PHA has good application prospects, its production cost is much higher than that of petroleum-derived plastic materials, thus limiting its large-scale production and application.

Method used

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  • Application of marinobacterium in preparation of polyhydroxyalkanoate
  • Application of marinobacterium in preparation of polyhydroxyalkanoate
  • Application of marinobacterium in preparation of polyhydroxyalkanoate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1, utilize acetic acid as carbon source to produce polyhydroxyalkanoate

[0073] 1. Aseptically prepare the seed solutions of three strains of M. nitratireducens, M. sediminicola and M. zhoushanense respectively.

[0074] (1) Activation of bacteria

[0075] Take a glycerol tube of strains stored in a -80°C refrigerator, streak and inoculate it on a TYS medium plate, and incubate at 37°C for 24 hours.

[0076] (2) Preparation of seed solution

[0077] Pick a single colony from the plate that completed step (1), inoculate it in liquid TYS medium, and cultivate it with shaking at 37°C and 200rpm for 12-16h.

[0078] 2. Prepare liquid TYS medium, add acetic acid to a final concentration of 10g / L, and adjust the pH to 7.5.

[0079] 3. The seed liquid obtained in step 1 (2) in Example 1 was inoculated respectively in liquid TYS medium containing 10 g / L acetic acid (to Acetic acid was added to the liquid TYS medium until the content of acetic acid was 10g / L (the...

Embodiment 2

[0089] Embodiment 2, utilize propionic acid as carbon source to produce polyhydroxyalkanoate

[0090] The specific operation is according to Example 1 of the present invention, the difference is that the medium in step 3 is a liquid TYS medium containing 10 g / L propionic acid. Step 5 detects poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV for short).

[0091] The PHBV output algorithm is: PHBV output = PHB output + PHV output, where:

[0092] PHB yield=(peak area of ​​PHB in sample / peak area of ​​internal standard in sample)×[(peak area of ​​internal standard in standard / peak area of ​​PHB in standard)×(mass of standard×0.866)] / mass of esterification of sample× dry cell weight

[0093] PHV yield=(peak area of ​​PHV in sample / peak area of ​​internal standard in sample)×[(peak area of ​​internal standard in standard / peak area of ​​PHV in standard)×(mass of standard×0.134)] / mass of esterification of sample× dry cell weight

[0094] Polymer content is defined as the ratio o...

Embodiment 3

[0100] Embodiment 3, utilize butyric acid as carbon source to produce polyhydroxyalkanoate

[0101] The specific operation is as in Example 1 of the present invention, except that the medium in step 3 is a liquid TYS medium containing 10 g / L butyric acid.

[0102] The dry cell weight and PHB accumulation of the three strains using butyric acid are shown in Table 3:

[0103] Table 3 The cell growth and PHB accumulation of three kinds of marine bacteria when using butyric acid as carbon source

[0104]

[0105] When butyric acid was used as the carbon source, the dry cell weights of M. nitratireducens, M. sediminicola and M. zhoushanense were 5.06g / L, 3.25g / L and 1.92g / L, respectively, and the polyhydroxyalkanoate accumulated in the cells was The yields of poly-3-hydroxybutyrate were 3.38g / L, 2.07g / L and 0.63g / L, respectively, and the mass fractions of PHB in dry cell weight were 66.81%, 58.39% and 32.49%.

[0106] In addition, utilize transmission electron microscope to ob...

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Abstract

The invention discloses an application of a marinobacterium in preparation of polyhydroxyalkanoate. The marinobacterium is applied to the preparation of polyhydroxyalkanoate. The invention further provides a method for preparing the polyhydroxyalkanoate. The method comprises the following steps that the marinobacterium is fermented and cultured by using a culture medium containing a carbon source to obtain a fermentation product; and the polyhydroxyalkanoate is obtained from the fermentation product. According to the application of a marinobacterium in preparation of the polyhydroxyalkanoate and the method, volatile fatty acid can be used as a carbon source for rapid growth and accumulation of polyhydroxyalkanoate. The volatile fatty acid is used as a carbon source, so that the fermentation cost of the polyhydroxyalkanoate is reduced, and great economic advantages and good industrial application prospects are achieved.

Description

technical field [0001] The invention relates to the application of sea bacterium, more specifically to the application of sea bacterium in preparing polyhydroxyalkanoate and a method for preparing polyhydroxyalkanoate, belonging to the field of microbiology and bioengineering. Background technique [0002] With the increasing depletion of petroleum resources and the intensification of the problem of "white pollution" caused by non-degradable plastic products using it as raw materials, renewable biomass resources are used instead of petroleum resources to produce biodegradable plastic materials needed by people in daily life , has received extensive attention from both scientific and industrial circles. Polyhydroxyalkanoate (English name polyhydroxyalkanoate, referred to as PHA) can be synthesized by many microorganisms in the cells under the condition of unbalanced growth and metabolism. It has material properties and biodegradability similar to plastics, and has broad appli...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/62C12R1/01
CPCC12N1/20C12P7/625
Inventor 李正军王梦汝郑国钧李弘飞陶观宝
Owner BEIJING UNIV OF CHEM TECH
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