Method for identifying deer hard antler button effective components for treating mastitis

Abstract

The invention discloses a method for identifying deer hard antler button effective components for treating mastitis. The method comprises the following steps: S1, setting deer hard antler button extracts with gradient concentration; S2, determining and screening the change level of inflammatory cytokines; S3, determining and screening out an effect peak value, and collecting a treating fluid corresponding to the effect peak value; S4, verifying a single-component peak, and determining the treating fluid with a verified effect peak value; and S5, determining chemical components of the effective components. According to the invention, deer hard antler button is selected, and detailed components are obtained through pretreatment, concentration gradient setting, immunoassay, enzyme-linked immunosorbent assay, real-time fluorescent quantitative PCR detection, immunoblotting analysis, fingerprint map, grey correlation analysis, verification of a single-component peak and non-targeted metabonomics of a liquid chromatograph-mass spectrometer, so that comprehensive judgment of efficacy and accurate identification are realized.

Description

technical field [0001] The invention relates to the technical field of identification of traditional Chinese medicine components, in particular to an identification method for effective components of antler discs for treating mastitis. Background technique [0002] Antler discs are the antler bases that fall off in the spring of the next spring after red deer or sika deer saw antlers. It contains mineral elements, amino acids, proteins, polypeptides, polysaccharides, lipids, cholesterol and sex hormones. In traditional animal Chinese medicine, deer antler discs have a long history of medicinal use. It not only has the effects of warming kidney yang, strengthening muscles and bones, promoting blood circulation and reducing swelling, but also has the magic of no inflammation, no suppuration, and no swelling from the deer horn discs that fall off naturally on living tissues. Biological phenomena and extensive folk applications all show that antler discs have good anti-inflammat...

AI Technical Summary

Problems solved by technology

[0003] Throughout modern medicine and scholars' scientific research on antler discs, for mastitis, so far, no pharmacological experiments and anti-inflammatory mechanism research have been conducted in strict scientific experiments, and no effective ingredients have been screened. Most of the existing studies on the efficacy of antler discs focus on the analysis and research of single components such as proteins and polypeptides. The corresponding extraction methods are also based on the chemical properties of the monomer components, selecting suitable solvents and conditions for extraction, and single components Although body composition is extremely beneficial for understanding and analyzing the chemical composition and pharmacological effects of medicines, it often ignores the correlation between medicinal ingredients and cannot better reflect the comprehensive effects of animal Chinese medicine ingredients to a certain extent. Sexual Chinese medicine, the efficacy of treating mumps, mammary gland hyperplasia, mastitis, etc. has withstood the test of thousands of years, and this is not just a certain monomer component that plays a role. Composition Theory" as evidence

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