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A kit and method for rapidly determining the sensitivity of citrus spider mites to acaricides

A technology of acaricide and spider mite, which is applied in the field of kits for rapid determination of the sensitivity of citrus spider mite to acaricide, which can solve the problems of strong technical expertise, long time-consuming, and heavy workload.

Active Publication Date: 2022-03-15
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have the following problems: one is strong technical expertise;

Method used

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  • A kit and method for rapidly determining the sensitivity of citrus spider mites to acaricides
  • A kit and method for rapidly determining the sensitivity of citrus spider mites to acaricides
  • A kit and method for rapidly determining the sensitivity of citrus spider mites to acaricides

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Embodiment 1

[0020] The diagnostic kit for rapid determination of the mite-killing sensitivity of citrus spider mite in this embodiment consists of a 50ml plastic centrifuge tube, clean and fresh bean leaves, a portable magnifying glass (60 times), and a No. 0 writing brush. The said plastic centrifuge tube is equipped with the acaricide of corresponding diagnostic dosage concentration.

[0021] The method of using the diagnostic kit for quickly determining the sensitivity of the citrus spider mite to the acaricide of the present invention is as follows: take the prepared diagnostic kit of the acaricide to the field, and pick 25 oranges of the same size with a No. 0 brush The female spider mites were transferred to bean leaves, put into a plastic centrifuge tube filled with liquid medicine (acaricide) for 5-10 seconds, observed with a portable magnifying glass after 1 hour, and the mortality rate was calculated.

[0022] 1. The concentration and dosage test of the medicinal solution in the...

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Abstract

The invention discloses a kit and a method for rapidly measuring the sensitivity of citrus spider mite field populations to acaricides in different regions and on host plants. A. Randomly select 5 plants from the field in the same area to collect adult mites of citrus red spider mites, pick up female adult mites of citrus red spider mite sensitive strains with a brush and transfer them to bean leaves, and put them into a centrifuge with gradient concentrations of acaricides. Tube, shake for 5-10s, so that the insect body contacts the acaricide. After taking out, cultivate and observe for 1 hour and calculate the mortality rate, draw the virulence regression curve, obtain the virulence regression equation, and calculate the LC of the citrus spider mite sensitive strain to the acaricide 99 , and get 2*LC 99 , with 2*LC 99 As an alternative concentration for the diagnostic dose, set the corresponding concentration gradient before and after the concentration to carry out the verification test, and select the dose value at which the mortality rate of citrus spider mite is 100% as the diagnostic dose; Transfer the adult red spider mite to the bean leaf, put it into a centrifuge tube containing a diagnostic dose of acaricide and shake it for 5-10 seconds, so that the insect body is exposed to the acaricide, after taking it out, culture and observe for 1 hour, and calculate the mortality rate to be tested Susceptibility of citrus spider mite to acaricides.

Description

technical field [0001] The invention belongs to the field of pest control, and in particular relates to a kit and a method for rapidly measuring the sensitivity of citrus spider mites to acaricides. Background technique: [0002] The citrus spider mite Panonychus citri (McGregor) belongs to the class Arachnida, the order Acarina, the family Tetranychidae, and the genus Panonychus (Mcgregor, 1948). Mites and red spider mites belong to the complete metamorphosis class of mites. The citrus spider mite is a polyphagous mite with worldwide distribution. It is mainly distributed in citrus producing areas such as Asia, Europe, Africa, and South America. It can feed on 112 species of host plants such as Rutaceae, Fabaceae, and Moraceae. It mainly damages citrus fruit trees (A.Migeon et al., 2012). Citrus spider mite mainly sucks the juice of leaves, twigs and fruits with the mouth needles of adults, nymphs or young mites. The damaged leaves form white spots, and the back of the le...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01M1/20A01K67/033
CPCA01M1/2011A01K67/033
Inventor 曾鑫年彭敏珊刘家莉
Owner SOUTH CHINA AGRI UNIV
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