Kit and method for rapidly determining sensitivity of panonychus citri to acaricide

Abstract

The invention discloses a kit and a method for rapidly determining sensitivity of panonychus citri field populations in different areas and host plants to an acaricide. The method comprises the following steps of A, randomly selecting five plants from the field of the same region to collect panonychus citri adult mites, picking female adult mites of panonychus citri sensitive strains by using a writing brush, transferring the female adult mites to bean leaves, putting the bean leaves into a centrifuge tube filled with the acaricide with gradient concentration, and shaking for 5-10 seconds to enable insect bodies to be in contact with the acaricide; after taking out, culturing and observing for 1h, calculating the death rate, drawing a toxicity regression curve, obtaining a toxicity regression equation, calculating to obtain LC99 of the panonychus citri sensitive strain to the acaricide, obtaining 2*LC99, taking 2*LC99 as a diagnosis dosage alternative concentration, and setting corresponding concentration gradients before and after the concentration to carry out a verification test; selecting a dosage value with the panonychus citri death rate of 100% as a diagnosis dosage; and B, picking panonychus citri female adult mites of the to-be-detected land with a writing brush, transferring the panonychus citri female adult mites to bean leaves, putting the bean leaves into a centrifuge tube containing a diagnostic dose of acaricide, shaking for 5-10 seconds to enable insect bodies to be in contact with the acaricide, taking out, culturing and observing for 1 hour, and calculating the death rate to judge the sensitivity of panonychus citri to the acaricide.

Description

technical field [0001] The invention belongs to the field of pest control, and in particular relates to a kit and a method for rapidly measuring the sensitivity of citrus spider mites to acaricides. Background technique: [0002] The citrus spider mite Panonychus citri (McGregor) belongs to the class Arachnida, the order Acarina, the family Tetranychidae, and the genus Panonychus (Mcgregor, 1948). Mites and red spider mites belong to the complete metamorphosis class of mites. The citrus spider mite is a polyphagous mite with worldwide distribution. It is mainly distributed in citrus producing areas such as Asia, Europe, Africa, and South America. It can feed on 112 species of host plants such as Rutaceae, Fabaceae, and Moraceae. It mainly damages citrus fruit trees (A.Migeon et al., 2012). Citrus spider mite mainly sucks the juice of leaves, twigs and fruits with the mouth needles of adults, nymphs or young mites. The damaged leaves form white spots, and the back of the le...

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Problems solved by technology

However, these methods have the following problems: one is strong technical expertise;

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