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A kind of method and its application of extracting apigenin from mountain celery

A kind of technology of apigenin and dihydro apigenin, applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, drug combinations, etc., can solve the problems of undisclosed apigenin, less excretion, uric acid cannot be excreted normally, and achieve Improve the yield and purity, improve the effect of reducing uric acid, and improve the effect

Active Publication Date: 2021-09-28
吉林浩泰健康产业发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are four main reasons for the accumulation of uric acid: the first is excessive consumption of high-purine foods; the second is a problem with purine metabolism in the body; the third is too little excretion; the fourth is that uric acid cannot be excreted normally
[0011] In the prior art, there is no report on the use of kaempferin for reducing uric acid

Method used

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  • A kind of method and its application of extracting apigenin from mountain celery
  • A kind of method and its application of extracting apigenin from mountain celery
  • A kind of method and its application of extracting apigenin from mountain celery

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A method for extracting apigenin from celery, comprising the steps of:

[0053] (1) Dry and pulverize the celery, add 5 times the amount of solvent A to extract, add 3% xylanase for enzymolysis for 10 minutes, and then add 5% glucosidase, the solvent A is ethylene glycol, iso A mixed solvent of propanol and water, the volume ratio of the three is 2:1:1, the extraction temperature is 30°C, the total extraction time is 1h, and the extract is concentrated to 1 / 5 of the original volume to obtain substance A;

[0054] (2) Substance A is successively added into equal volumes of petroleum ether, chloroform and ethyl acetate for extraction, the extraction layers of chloroform and ethyl acetate are collected, dried respectively, and combined to obtain a crude product;

[0055] (3) The crude product is dissolved with 30% ethanol of 3 times the amount, and the D101 macroporous adsorption resin is used for adsorption, and the solvent D is used for elution, and the solvent D is a mi...

Embodiment 2

[0058] A method for extracting apigenin from celery, comprising the steps of:

[0059] (1) Dry and pulverize the celery, add 10 times the amount of solvent A to extract, add 8% xylanase for enzymolysis for 25min, then add 10% glucosidase, the solvent A is ethylene glycol, iso A mixed solvent of propanol and water, the volume ratio of the three is 4:1:3, the extraction temperature is 40°C, the total extraction time is 2h, and the extract is concentrated to 1 / 8 of the original volume to obtain substance A;

[0060] (2) Substance A is successively added into equal volumes of petroleum ether, chloroform and ethyl acetate for extraction, the extraction layers of chloroform and ethyl acetate are collected, dried respectively, and combined to obtain a crude product;

[0061] (3) The crude product is dissolved with 5 times of 90% ethanol, adsorbed by D101 macroporous adsorption resin, and eluted by solvent D, which is a mixed solvent of chloroform and methanol, and the volume ratio of...

Embodiment 3

[0064] A method for extracting apigenin from celery, comprising the steps of:

[0065] (1) Dry and pulverize the celery, add 8 times the amount of solvent A to extract, add 5% xylanase for enzymolysis for 15min, then add 7% glucosidase, the solvent A is ethylene glycol, iso A mixed solvent of propanol and water, the volume ratio of the three is 3:1:2, the extraction temperature is 35°C, the total extraction time is 1.5h, and the extract is concentrated to 1 / 6 of the original volume to obtain substance A;

[0066] (2) Substance A is successively added into equal volumes of petroleum ether, chloroform and ethyl acetate for extraction, the extraction layers of chloroform and ethyl acetate are collected, dried respectively, and combined to obtain a crude product;

[0067] (3) The crude product is dissolved with 4 times of 60% ethanol, adsorbed by D101 macroporous adsorption resin, and eluted by solvent D, which is a mixed solvent of chloroform and methanol, and the volume ratio of...

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Abstract

The invention relates to the technical field of plant extraction, in particular to a method for extracting apigenin from celery and its application. The method comprises the following steps: (1) drying and pulverizing the celery, adding solvent A for extraction, and extracting The liquid is concentrated to obtain substance A; (2) substance A is extracted with solvent B, and the extracted solvent B layer is dried to obtain a crude product; (3) the crude product is dissolved with solvent C, and adsorbed by a macroporous adsorption resin, Use solvent D for elution to obtain the eluent; (4) concentrating and crystallizing the eluent to obtain the final product. The invention has the advantages of high yield and high purity, and the combined use of kaempferin and other effective ingredients has the advantages of remarkable uric acid-lowering effect.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for extracting apigenin from celery and its application. Background technique [0002] Celery is a perennial herb of Umbelliferae and the genus Celery. Celery has both medicinal and edible value. Shanqin has the effects of mainly treating rheumatic arthralgia, soreness of the waist and knees, cold headache, carbuncle sores, swelling and pain. Celery is a high-grade rare vegetable in celery. The nutrient content of wild celery is also higher in wild vegetables, and the seedlings of wild celery can be used as wild vegetables in spring. Its nutritional effects are as follows: (1) Celery has high iron content, which can supplement the loss of women's menstrual blood. Eating it can prevent pale, dry and dull complexion, and can make eyes bright and hair black and shiny. (2) Celery is a high-fiber food. It produces a lignin or intestinal fat substance through intes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30C07D311/40A61K31/352A61K31/519A61P19/06A61K36/238A61K31/353A61K31/37
CPCA61K31/352A61K31/353A61K31/37A61K31/519A61K36/238A61K2236/19A61K2236/333A61K2236/55A61P19/06C07D311/30C07D311/40A61K2300/00
Inventor 李雪殷金龙殷金玲董志杰
Owner 吉林浩泰健康产业发展股份有限公司
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