A kind of method for preparing peanut meal

A technology of peanut meal and enzymolysis, which is applied in the fields of animal feed and bioengineering, can solve the problems of reduced disease resistance of fish, adverse effects, and liver cell damage in growing and fattening pigs, so as to improve comprehensive antioxidant properties and reduce arginine. The effect of improving acid content, overall protein quality

Active Publication Date: 2022-04-29
JIANGNAN UNIV
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Problems solved by technology

Although this method can degrade the anti-nutritional factors and toxic substances in the feed cake to a certain extent and improve the nutrients in the feed, but because the enzyme itself is also a kind of protein, it is easy to be decomposed by some enzymes produced by microorganisms, and there are many problems. massive loss
Although the current research has increased the proportion of animal-limiting amino acids (such as lysine and methionine) to a certain extent, it has not reduced the excessively high arginine content in peanut meal, and amino acid antagonism still affects the normal growth of livestock metabolism
[0004] In addition, artificially synthesized antioxidants are generally added to the feed, such as ethoxyquinoline (EMQ), di-tert-butylhydroxytoluene (BHT), tert-butylhydroxyanisole (BHA), etc., to enhance the animal's resistance Oxidation performance, however, these synthetic feed antioxidants have safety assessment issues. Studies have shown that high doses of ethoxyquin can cause liver cell damage in growing and fattening pigs, and long-term feeding of fish with EMQ can even make fish resistant to disease Reduced ability
In addition, because BHT has adverse effects on the body's liver, spleen, lungs, etc.

Method used

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  • A kind of method for preparing peanut meal

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Embodiment 1

[0043] (1) Preparation of peanut meal enzymatic hydrolyzate

[0044] Peanut meal (the peanut meal is completely crushed and passed through a 40-mesh sieve) is mixed with water at a ratio of 1:5 (w / w), and 0.1% cellulase (purchased from Baiyin Sanuo Biotechnology Co., Ltd.) and phytic acid are added respectively Enzyme (provided by Wuhan Xinhuayang Biological Co., Ltd.), enzymolysis at 50°C for 1h, inactivated in boiling water bath for 10min, supplemented with distilled water to 250mL, and then acid protease (provided by Weifang Kangdien Biotechnology Co., Ltd.) for a second After enzymatic hydrolysis and enzyme inactivation, adjust the pH to 5.5.

[0045] Optimization of the secondary enzymatic hydrolysis conditions, changing the conditions of acidic protease enzymatic hydrolysis of peanut meal, and investigating the effect of the degree of protein hydrolysis of peanut meal, specifically changing the substrate enzyme activity, substrate mass fraction, enzymatic hydrolysis temp...

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Abstract

The invention discloses a method for preparing peanut meal, which belongs to the technical field of animal feed and bioengineering. The peanut meal is used as the main raw material, and the peanut meal is prepared by combining enzymatic hydrolysis and microbial fermentation. The arginine content of the prepared peanut meal is reduced, effectively balancing the content of amino acids, and producing a variety of beneficial metabolites such as enzymes, functional polypeptides, lactic acid and lactate, which solves the problem of amino acid imbalance and poor palatability of peanut meal , the problem of low nutritional value caused by high molecular protein and low digestibility. At the same time, it can also improve the comprehensive antioxidant performance of peanut meal, so that the DPPH, hydroxyl radical, superoxide anion scavenging rate and iron reducing power in peanut meal are all improved, which improves the utilization value of peanut meal and is beneficial to reduce free radicals in animals. , Improve the immunity of animals, improve the cost performance of feed and the efficiency of livestock and poultry breeding.

Description

technical field [0001] The invention relates to a method for preparing peanut meal, belonging to the technical fields of animal feed and bioengineering. Background technique [0002] Peanut meal is a by-product of peanuts deoiled by hot pressing. It is rich in nutrients, and its protein content is as high as 40%-50%. It has a complete range of amino acids and is a potential high-quality protein feed. Although peanut meal has a high protein content, the amino acid imbalance problem still limits its efficient utilization in animal farming. For example, the ratio of arginine to lysine in peanut meal is as high as 3.45, and excessive intake of arginine leads to The generation of amino acid antagonism in animals inhibits the absorption of the limiting amino acid (lysine) in animals, causing them to be lost in large quantities in urine and hindering the normal growth and reproduction of animals. Compared with high-quality protein feed raw materials such as fishmeal and soybean me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/14A23K10/12A23K10/37
CPCA23K10/14A23K10/12A23K10/37A23V2400/121Y02P60/87
Inventor 蔡国林陆健周佳慧李晓敏吴殿辉余培斌
Owner JIANGNAN UNIV
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