Method for preparing peanut meal

A technology of peanut meal and enzymolysis, which is applied in the fields of animal feed and bioengineering, can solve the problems of reduced disease resistance of fish, adverse effects, and liver cell damage in growing and fattening pigs, so as to improve comprehensive antioxidant properties and reduce arginine. The effect of improving acid content, overall protein quality

Active Publication Date: 2021-04-02
JIANGNAN UNIV
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Problems solved by technology

Although this method can degrade the anti-nutritional factors and toxic substances in the feed cake to a certain extent and improve the nutrients in the feed, but because the enzyme itself is also a kind of protein, it is easy to be decomposed by some enzymes produced by microorganisms, and there are many problems. massive loss
Although the current research has increased the proportion of animal-limiting amino acids (such as lysine and methionine) to a certain extent, it has not reduced the excessively high arginine content in peanut meal, and amino acid antagonism still affects the normal growth of livestock metabolism
[0004] In addition, artificially synthesized antioxidants are generally added to the feed, such as ethoxyquinoline (EMQ), di-tert-butylhydroxytoluene (BHT), tert-butylhydroxyanisole (BHA), etc., to enhance the animal's resistance Oxidation performance, however, these synthetic feed antioxidants have safety assessment issues. Studies have shown that high doses of ethoxyquin can cause liver cell damage in growing and fattening pigs, and long-term feeding of fish with EMQ can even make fish resistant to disease Reduced ability
In addition, because BHT has adverse effects on the body's liver, spleen, lungs, etc.

Method used

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  • Method for preparing peanut meal

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Embodiment 1

[0043] (1) Preparation of peanut meal enzymatic hydrolyzate

[0044] Peanut meal (the peanut meal is completely crushed and passed through a 40-mesh sieve) is mixed with water at a ratio of 1:5 (w / w), and 0.1% cellulase (purchased from Baiyin Sanuo Biotechnology Co., Ltd.) and phytic acid are added respectively Enzyme (provided by Wuhan Xinhuayang Biological Co., Ltd.), enzymolysis at 50°C for 1h, inactivated in boiling water bath for 10min, supplemented with distilled water to 250mL, and then acid protease (provided by Weifang Kangdien Biotechnology Co., Ltd.) for a second After enzymatic hydrolysis and enzyme inactivation, adjust the pH to 5.5.

[0045] Optimization of the secondary enzymatic hydrolysis conditions, changing the conditions of acidic protease enzymatic hydrolysis of peanut meal, and investigating the effect of the degree of protein hydrolysis of peanut meal, specifically changing the substrate enzyme activity, substrate mass fraction, enzymatic hydrolysis temp...

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Abstract

The invention discloses a method for preparing peanut meal, and belongs to the technical field of an animal feed and bioengineering. The peanut meal is used as a main raw material, and the peanut mealis prepared by utilizing a method of combining enzymolysis and microbial fermentation. The arginine content of the prepared peanut meal is reduced, the content of amino acid is effectively balanced,beneficial metabolites such as various enzymes, functional polypeptide, lactic acid, lactate and the like are generated, and the problem of low nutritional value caused by imbalance of the amino acid,poor palatability, high macromolecular protein content, low digestibility and the like of the peanut meal is solved. Meanwhile, the comprehensive oxidation resistance of the peanut meal also can be improved, so that the scavenging rate of DPPH, hydroxyl free radicals and superoxide anions in the peanut meal and the iron reducing power are both improved; the utilization value of the peanut meal isimproved; free radicals in animal bodies are reduced; the immunity of the animal bodies is improved; and the cost performance of the feed and the livestock and poultry breeding benefits are improved.

Description

technical field [0001] The invention relates to a method for preparing peanut meal, belonging to the technical fields of animal feed and bioengineering. Background technique [0002] Peanut meal is a by-product of peanuts deoiled by hot pressing. It is rich in nutrients, and its protein content is as high as 40%-50%. It has a complete range of amino acids and is a potential high-quality protein feed. Although peanut meal has a high protein content, the amino acid imbalance problem still limits its efficient utilization in animal farming. For example, the ratio of arginine to lysine in peanut meal is as high as 3.45, and excessive intake of arginine leads to The generation of amino acid antagonism in animals inhibits the absorption of the limiting amino acid (lysine) in animals, causing them to be lost in large quantities in urine and hindering the normal growth and reproduction of animals. Compared with high-quality protein feed raw materials such as fishmeal and soybean me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/14A23K10/12A23K10/37
CPCA23K10/14A23K10/12A23K10/37A23V2400/121Y02P60/87
Inventor 蔡国林陆健周佳慧李晓敏吴殿辉余培斌
Owner JIANGNAN UNIV
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