Application of tau protein and genes thereof as drug targets in preparation of drugs for treating diabetes mellitus

A diabetes and protein technology, which is applied in the field of diabetes treatment, can solve the problems of few targets, affect the glucose metabolism system, and high risk of complications, and achieve the effect of promoting extracellular secretion and reducing blood sugar levels

Active Publication Date: 2021-03-30
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These data suggest that diabetes not only affects the body's glucose metabolism system, but also has a high risk of complications
[0005] But at present, insulin is usually used mainly for diabetes, and there are fewer targets, and more therapeutic targets are still needed for diabetes

Method used

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  • Application of tau protein and genes thereof as drug targets in preparation of drugs for treating diabetes mellitus
  • Application of tau protein and genes thereof as drug targets in preparation of drugs for treating diabetes mellitus
  • Application of tau protein and genes thereof as drug targets in preparation of drugs for treating diabetes mellitus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Quantitative proteomics analysis of islet cells INS-1(tau-KD) cells

[0060] Protein is the main bearer of life activities, and proteomics can study the expression level of all proteins and the interaction between proteins at the overall level. In order to explore the importance of tau protein in the process of insulin secretion from the overall protein level, the embodiment of the present invention constructed islet INS-1 (rat insulinoma cells) with reduced tau protein level for quantitative proteomic analysis (N=4) .

[0061] A total of 5933 proteins were quantified in the quantitative proteomics analysis. From the quantitative proteins, 100 up-regulated and down-regulated proteins with the largest fold difference were selected for overall protein expression heat map analysis. The heat map analysis showed that each replicate sample in the WT group and tau-KD group showed good uniformity, indicating that this The data has good repeatability and reliability, and can b...

Embodiment 2

[0063] Changes of tau protein level during insulin secretion

[0064] 2.1 The level of tau protein is down-regulated in a glucose-dependent manner in islet cells

[0065]In order to determine the change of tau protein level in the process of insulin secretion in vitro, this example utilizes different concentrations of glucose: low concentration (2.8mM), medium concentration (11.1mM) and high concentration (16.7mM) glucose to stimulate islet cell INS-1 (Rat islet cell tumor cells) were subjected to glucose-stimulated insulin secretion test (GSIS). After the GSIS experiment, Western Blot was used to detect the change of tau protein expression level in INS-1 cells. The experimental results showed that with the increase of glucose concentration, the expression level of tau protein was gradually down-regulated ( image 3 ). This phenomenon indicated that the level of tau protein changed during the insulin secretion process of islet cells, suggesting that tau protein may be invol...

Embodiment 3

[0069] Effects of decreased tau protein level on insulin secretion and glucose tolerance

[0070] 3.1 The reduction of tau protein level increases the secretion of insulin in INS-1 cells

[0071] After confirming the significant change of tau protein level in the process of insulin secretion, this implementation uses the INS-1tau-KD islet cell model to explore the regulation of tau protein on insulin secretion. First, the reduction of tau protein level in INS-1 cells was verified by Western blot ( Figure 5 Middle a). INS-1 cells can secrete insulin under the stimulation of glucose, so in order to determine whether the reduction of tau protein level will affect the secretion of insulin in INS-1 cells, we conducted a glucose-stimulated insulin secretion experiment (GSIS) using INS-1tau-KD cells , GSIS results showed that, compared with WT group, INS-1(tau-KD) cells significantly increased insulin secretion under the stimulation of low-concentration glucose (2.8mm) and high-co...

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Abstract

The invention discloses tau protein and application of a gene of the tau protein as a drug target in preparation of a drug for treating diabetes, and relates to the technical field of diabetes treatment. The invention discloses an application of a reagent taking tau protein or tau gene as a target point in preparation of a medicine for preventing or treating diabetes mellitus. Embodiment of the invention creatively shows that tau protein has an important regulation and control effect in insulin secretion, and diabetes mellitus can be treated by targeting the tau protein or the tau gene. The invention provides a new thought and strategy for diabetes treatment and drug research and development or screening.

Description

technical field [0001] The invention relates to the technical field of diabetes treatment, in particular to the application of tau protein and its gene as a drug target in the preparation of medicines for treating diabetes. Background technique [0002] Diabetes is a common chronic disease. About 10% of the adults in the world suffer from diabetes, and its complications also increase the economic and medical burden. Diabetes spending in the United States reached $465 billion in 2011, accounting for 11% of all health care costs for adults. China is the country with the largest number of diabetic patients in the world, and it is still increasing year by year. The prevalence rate in my country was 0.67% in 1980, and by 2013, 11% of adults (about 154 million) in my country have suffered from varying degrees. of diabetes. These data show that the incidence of diabetes in the world has increased significantly, and has caused serious medical and economic burdens. [0003] Diabete...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61P3/10
CPCA61K45/00A61P3/10
Inventor 雷鹏庹清章李小兰
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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