Method for establishing nerve root type cervical spondylosis animal model

Abstract

The invention belongs to the technical field of medical treatment, and particularly relates to a method for establishing a nerve root type cervical spondylosis animal model. The method comprises the following steps: obtaining an anesthetized object and a rat subjected to abdominal cavity anesthesia; taking a subclavian incision and free subcutaneous tissue on the right side of the rat, sequentially separating muscles and fascias according to the thoracic muscle fiber deformation direction, exposing and separating brachial plexus nerves and subclavian arteries and veins, and obtaining a targetposition; cutting blunt separation tissues by using tweezers and small tissues; protecting vein vessels, and increasing the visual field range; performing blunt separation tto protect the inner artery; ligating the brachial plexus nerve, wherein the catgut made of 4-0 chromium and the clavicle are fixed together, the distance between every two adjacent ligation positions is 1 mm, and ligation is conducted three times; stitching the incision; the method has the advantages that stable nerve root compression can be provided, interference of other factors is avoided, operation is easy, a large-scale nerve root type cervical spondylosis animal model can be established, and research on pathogenesis of nerve root type cervical spondylosis is facilitated.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for an animal model of nerve root cervical spondylosis. Background technique [0002] Cervical spondylotic radiculopathy refers to a certain degree of pain, numbness, loss of sensation, and changes in reflexes in the areas dominated by cervical spine degeneration, compression of nerve roots, and other factors. The main symptom of cervical spondylotic radiculopathy is root pain [1], accounting for 50%-60% of the total number of cervical spondylosis [2]. At present, the pathological mechanism of cervical spondylotic radiculopathy has not been studied clearly. At the same time, the animal model of cervical spondylotic radiculopathy has no clear and unified construction method and recognized effective evaluation system. Changed standardized animal models and model evaluation systems are necessary prerequisites for basic research on the disease. [0003] At present, animal...

AI Technical Summary

Problems solved by technology

Although the nylon thread in the experiment can increase tissue adhesion after being treated with ethanol and polylysine solution, it still cannot guarantee that the nylon thread will maintain a fixed and continuous compression on the cervical nerve root without displacement and the nerve root compression will disappear. or cause spinal cord compression

Because the degree of entrapment by the nylon thread is relatively light, and the self-repair ability of rat nerves is strong, the sensory function will basically recover in about 2 weeks, so this scheme is only suitable for acute phase research.

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