Method and device for synthesizing trehalose by ultrasonic-assisted enzyme

Abstract

The invention relates to the field of enzyme preparation, and particularly discloses a method and device for synthesizing trehalose by ultrasonic-assisted enzyme. The method comprises the following steps: adding water, starch and alpha-amylase into a reaction kettle, and performing mixing; regulating the pH value of the mixture; adding anhydrous calcium chloride into the mixture; starting a stirring shaft of the reaction kettle stirring the mixture, a plurality of ultrasonic transducers, a heater and a temperature detector, starting a circulation path to outward transfer, crush and filter themixture at the bottom of the reaction kettle, and sending the mixture back into the reaction kettle; heating the mixture by stages and performing insulation; and stopping heating when the liquefactionDE value is 15, performing stirring and circulating cooling the mixture to the normal temperature, regulating the pH value of the mixture, adding pullulanase, MTSase, MTHase and alpha / beta-CGTase into the mixture, performing reheating, preserving the temperature at 45 DEG C, regulating the pH value of the mixture, adding glucoamylase into the mixture, and performing mixing to finally obtain a product. The ultrasonic-assisted enzyme is used for promoting synthesis of trehalose to achieve a remarkable effect for improving the conversion rate of trehalose synthesized by using rice starch as a basic raw material.

Description

technical field [0001] The invention belongs to the field of enzyme preparation, in particular to a method and device for ultrasonic-assisted enzyme synthesis of trehalose. Background technique [0002] Some insects and plants that live in desert areas for many years are almost dehydrated and dehydrated in the high temperature at noon, and are in a state of physiological suspended animation, but they can be revived after a few hours after rainfall replenishes water. Scholars at the University of Cambridge in the United Kingdom have shown that this resurrection phenomenon is due to the presence of high concentrations of trehalose in their bodies. In addition, some organisms such as frogs can survive under severe cold conditions, and the important reason is also the protective effect of trehalose on cells. The stress tolerance of many organisms under stress (such as starvation, high temperature, freezing, desiccation, hypertonicity, radiation, toxic substances, etc.) is direc...

AI Technical Summary

Problems solved by technology

Due to the low yield of trehalose and complex production process, the product price is still high, which limits the popularization and application of trehalose. How to increase the yield of trehalose, how to improve the production method, and how to reduce the production cost are important topics for the study of trehalose.

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