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Application of antioxidant in-vitro maturation of animal oocytes

An oocyte, in vitro mature technology, applied in the field of embryo engineering, can solve the problems of uneven egg quality, inability to obtain embryo sources, and low oocyte development ability.

Active Publication Date: 2021-03-19
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, oocytes obtained from either hormonal superovulation or abattoirs were significantly less developed than oocytes matured in vivo
The quality of these eggs is uneven, resulting in the inability to obtain a more stable source of embryos, which is the main defect of the current technology

Method used

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  • Application of antioxidant in-vitro maturation of animal oocytes
  • Application of antioxidant in-vitro maturation of animal oocytes
  • Application of antioxidant in-vitro maturation of animal oocytes

Examples

Experimental program
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Effect test

Embodiment 1

[0022] (1) Collection of adult sheep oocytes

[0023] After the ovaries were cut at the slaughterhouse, they were stored in saline at 37°C and shipped back to the experiment within two hours. The oocyte / cumulus cell complexes (COCs) in the ovary were aspirated with a 20G needle disposable syringe containing 2 mL of oocyte aspirating solution.

[0024] (2) Collection of lamb oocytes

[0025] Inject FSH (Ningbo Sansheng) six times for 4-8 week-old female lambs for 3 consecutive days, each injection of 45 mg, with an interval of 12 hours each time. The first injection of FSH was simultaneously injected with 330IU PMSG (Ningbo Sansheng). Surgical egg collection was performed 12 hours after the last FSH injection. Oocyte / cumulus cell complexes (COCs) in the ovary were aspirated in vivo with a 20G needle disposable syringe containing 2mL oocyte suction solution.

[0026] (3) In vitro maturation of oocytes

[0027] The selected COCs were washed three times in the oocyte suction ...

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Abstract

The invention discloses application of an antioxidant in-vitro maturation of animal oocytes. A method for improving the in-vitro maturation of the animal oocytes, provided by the invention, comprisesthe step of adding idebenone into an in-vitro maturation solution of the animal oocytes. The invention further provides the application of the idebenone in improvement of the in-vitro maturation of the animal oocytes. Specifically, the concentration of the idebenone in the in-vitro maturation solution of the animal oocytes is 10 to 30 microns, and more specifically, the concentration can be 20 microns. According to the method, in-vitro culture conditions of the oocytes are optimized, and the embryonic development capacity of the oocytes is improved. The method is simple and feasible, and materials are convenient to obtain, so that the method has an important application value.

Description

technical field [0001] The invention belongs to the field of embryo engineering and relates to the application of an antioxidant in the in vitro maturation of animal oocytes. Background technique [0002] Animal embryo in vitro production technology is to collect mammalian oocytes from ovarian follicles, and carry out a series of technical processes such as in vitro maturation of oocytes in culture medium, in vitro fertilization, and in vitro culture of embryos. Now it has become increasingly mature and has become a Important animal reproduction biotechnology. This technology is generally used for pure multiplication and population expansion, increasing the number of breeding animals; shortening the breeding cycle of livestock, accelerating breed improvement; transplanting to produce twins, improving production efficiency; preserving excellent breed resources, etc. Therefore, this technology is a rapid breeding technology with great application value. [0003] However, ooc...

Claims

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Application Information

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IPC IPC(8): C12N5/075
CPCC12N5/0609C12N2501/999C12N2501/31C12N2501/392
Inventor 侯健田浩刘克雄
Owner CHINA AGRI UNIV
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