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A pharmaceutical composition for preventing and treating cancer and its application

A composition and drug technology, applied in the field of biomedicine, can solve the problems of lack of effective treatment methods and unclear molecular mechanism of cancer, and achieve the effects of inhibiting glycolysis, promoting cell growth, and inducing cell apoptosis.

Active Publication Date: 2021-12-17
THE FIRST AFFILIATED HOSPITAL OF JINAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the technical problems in the prior art that the molecular mechanism of the occurrence, development and metastasis of cancer, especially prostate cancer, is not clear enough and lacks effective treatment methods, so as to study the mechanism of the occurrence and development of prostate cancer. Cancer offers a new means of prevention and treatment

Method used

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  • A pharmaceutical composition for preventing and treating cancer and its application
  • A pharmaceutical composition for preventing and treating cancer and its application
  • A pharmaceutical composition for preventing and treating cancer and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1 Correlation between expression levels of ANT2 and miR-137 and prostate cancer

[0078] (1) Collect surgically resected prostate cancer tissues and paracancerous tissues from prostate cancer patients, and sign an informed consent form with the patients;

[0079] (2) Divide each tissue sample into two parts, one part is frozen in liquid nitrogen within 30 minutes of ex vivo, and then transferred to -80°C; the other part is stored in 3.7% paraformaldehyde;

[0080](3) The samples at -80°C were used for protein extraction and Western blot (WB) and qPCR experiments; the samples in paraformaldehyde were used for pathological sections and subsequent immunohistochemical staining.

[0081] Pathological diagnosis was based on TNM staging in accordance with the staging criteria established by the American Joint Cancer Society (AJCC). Collect clinical follow-up data: age, gender, clinical grade, recurrence and metastasis, radiotherapy and chemotherapy, pathological type,...

Embodiment 2

[0089] Example 2 Effects of ANT2 and ANT2 Inhibitors on the Proliferation and Apoptosis of Prostate Cancer Cells

[0090] RNA was extracted from prostate cancer cells (DU145 and LNCaP cells) and reverse transcribed, ANT2 cDNA was amplified by PCR and constructed into a related vector, sequenced and protein expression verified, and the overexpression plasmid of ANT2 was obtained, and its effectiveness was verified by WB detection and specificity, the results are as follows Figure 7 shown.

[0091] Subsequently, different concentrations of ANT2 were overexpressed, treated for 24, 48 and 72 hours respectively, and the cells were detected by CCK-8, and the results were as follows Figure 8-9 As shown, it can be seen that after the overexpression of ANT2 gene, the proliferation ability of DU145 and LNCaP cells was significantly enhanced.

[0092] Further, siRNA (siANT2) was designed and synthesized for the mRNA sequence of Human ANT2, wherein the siANT2 sequence is GGUGACUGCCUGG...

Embodiment 3

[0094] Example 3 Effects of ANT2 and ANT2 inhibitors on the migration and invasion of prostate cancer cells

[0095] DU145 and LNCaP cells were selected for cell migration and invasion experiments. qPCR and WB were used to detect the expression of MMPs and TIMPs-related genes in the cells. Experimental detection of cell invasion ability. The result is as Figure 15-21 shown.

[0096] in Figure 15-17 It shows that after overexpressing the ANT2 gene, the migration and invasion abilities of DU145 cells and LNCaP cells are significantly enhanced; and after silencing the ANT2 gene with siANT2, the migration and invasion abilities of DU145 and LNCaP cells are significantly reduced, and the difference is statistically significant. significance.

[0097] Depend on Figure 18 It can be seen that after the ANT2 gene is silenced, the expression of Bax is low, the expression of Bcl-2 is high, and the expression of Caspase-3 is superhigh, indicating that the silencing of the ANT2 gen...

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Abstract

The invention provides a pharmaceutical composition for preventing and treating cancer, especially prostate cancer. The invention fully reveals the function of ANT2 protein in prostate cancer cells and the target genes regulated by it, clarifies the new mechanism of ANT2 regulating prostate cancer, and finds the key target for inhibiting prostate cancer. The present invention has carried out in-depth research on the specific mechanism of ANT2 in the process of prostate cancer occurrence, development, migration, invasion, apoptosis, etc., clarified the regulatory effect of miR-137 on the expression of ANT2, and determined the effect of miR-137 and ANT2 on prostate cancer. Effects on cell apoptosis, proliferation, migration and invasion. The invention clarifies the occurrence and development mechanism of prostate cancer completely, provides a new idea for the development of related drugs in the later stage, and clinical diagnosis and treatment, and has great social significance and market prospects.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a pharmaceutical composition for preventing and treating cancer and its application, in particular to a pharmaceutical composition for preventing and treating prostate cancer and its application. Background technique [0002] Existing studies have found that abnormal mitochondrial energy metabolism is closely related to tumorigenesis. In tumor cells, mitochondrial aerobic respiration is inhibited or destroyed due to poorly differentiated cells or environmental factors, and the glycolytic pathway takes its place. This phenomenon, known as the Warburg effect, was proposed as early as the 1920s and has been confirmed in an increasing number of tumor types. The energy metabolism of tumor cells has recently received great attention, and related proteins are considered as new tumor markers. Understanding the energy metabolism of tumor cells is of great significance for early diagnosis and tar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61K31/7105A61P35/00
CPCA61K45/00A61K31/7105A61P35/00
Inventor 潘斌李宇同陈果卓育敏
Owner THE FIRST AFFILIATED HOSPITAL OF JINAN UNIV
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