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Biological metabonomics analysis method for screening antioxidant active substances

An anti-oxidation activity and anti-oxidation technology, applied in the field of metabolomics analysis, can solve the problems of long cycle, high cost, and poor system

Inactive Publication Date: 2021-03-16
JIANGNAN UNIV
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Problems solved by technology

[0004] In order to solve the technical problems such as high cost, long period, and poor system of using mammals to screen antioxidant active compounds and evaluate their mechanism of action, the present invention provides a method for evaluating the antioxidant effect of active compounds using Caenorhabditis elegans as an antioxidant model. method

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  • Biological metabonomics analysis method for screening antioxidant active substances
  • Biological metabonomics analysis method for screening antioxidant active substances
  • Biological metabonomics analysis method for screening antioxidant active substances

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Embodiment 1

[0035] (1) Experimental grouping: normal control group, model group, and three anthocyanin pretreatment groups, including cyanidin, delphinidin, and mallow pigment-3-arabinoside, a total of 5 groups, with 7 experiments in each group Level.

[0036] (2) Cultivation of Caenorhabditis elegans: Wild-type Caenorhabditis elegans is cultured on NGM plates coated with Escherichia coli OP50, and eggs are collected during the oviposition period for synchronization. The worms were eluted from the plate and collected, and the density of Caenorhabditis elegans was adjusted to 2500-3000 / mL.

[0037] (3) Induction treatment: Caenorhabditis elegans at the L2 stage was evenly added to a 6-well plate at the above-mentioned density of 1 mL / well, and OP50 with a final concentration of 200 mg / mL was provided as food at the same time, and the final concentration was added to the anthocyanin pretreatment group 50 μM (prepared in sterile water) of the above three anthocyanins, the induction group an...

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Abstract

The invention discloses a biological metabonomics analysis method for screening antioxidant active substances, and belongs to the technical field of metabonomics analysis. Metabolites of caenorhabditis elegans samples are detected and analyzed by adopting a GCTOF / MS technology. ChromaTOF software and MS DIAL software carry out data conversion, calibration of a data baseline, peak alignment, identification, peak area calculation and the like, and normalize the peak area. Multivariate statistical analysis on five groups of caenorhabditis elegans samples including a normal control group, a modelgroup and three anthocyanin pretreatment groups are carried out, and biological metabolites with remarkable differences are screened. 15, 8, 9 and 14 different metabolites with antioxidant action mechanisms are respectively obtained through screening and checking, a model group and three anthocyanin pretreatment groups, recognition and high-throughput analysis of the antioxidant action mechanismsare realized by utilizing callback change of abundance of the different metabolites, and a metabolite path is visualized.

Description

technical field [0001] The invention relates to a biological metabolomics analysis method for screening antioxidant active substances, and belongs to the technical field of metabolomics analysis. Background technique [0002] Anthocyanins are a class of natural pigments in flavonoids, widely used in food, cosmetics, biomedicine and other fields. There are 6 kinds of anthocyanins commonly found in plants, and 5 kinds of anthocyanins are detected in blueberries, including cyanidin, mallow pigment, petunienin, paeoniflorin, and delphinidin. Anthocyanins are also common ingredients in human diets, and have various functional effects, such as anti-oxidation, anti-aging, anti-inflammation, etc., and thus have been widely used in food and medical fields. A large number of research results have shown that the antioxidant activity of anthocyanins is related to their chemical structure and even their glycoside forms. However, most previous studies on the antioxidant efficacy of anth...

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Application Information

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IPC IPC(8): G01N30/08G01N30/88
CPCG01N30/08G01N30/88
Inventor 孙秀兰叶永丽孙嘉笛纪剑张银志
Owner JIANGNAN UNIV
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