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Lactobacillus fermentum with weight reducing function and application of lactobacillus fermentum

A technology of Lactobacillus fermentum and Lactobacillus plantarum, applied in the field of microorganisms, can solve the problems of lowering serum leptin and achieve the effects of promoting secretion, inhibiting fat synthesis, and reducing weight

Active Publication Date: 2021-03-16
HANGZHOU WAHAHA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Lactobacillus fermentum that has the function of reducing serum leptin has not been found so far

Method used

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  • Lactobacillus fermentum with weight reducing function and application of lactobacillus fermentum
  • Lactobacillus fermentum with weight reducing function and application of lactobacillus fermentum
  • Lactobacillus fermentum with weight reducing function and application of lactobacillus fermentum

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Effect test

Embodiment 1

[0055] Embodiment 1: the biological property of lactobacillus fermentum WHH3906

[0056] The strain provided by the present invention has been identified as Lactobacillus fermentum, named as WHH3906 strain, and was preserved at the General Microorganism Culture Collection Center of China Microbiology Culture Collection Management Committee on March 13, 2020, and the microorganism preservation number is CGMCC NO.19472.

[0057] The bacterial strain Lactobacillus fermentum WHH3906 provided by the present invention is isolated from ghee collected in the Tibet Autonomous Region of my country.

[0058] The biological properties of bacterial strain Lactobacillus fermentum WHH3906 provided by the present invention are as follows:

[0059] Morphological characteristics: In MRS agar medium, the growth form is a milky white round colony, the surface is smooth and moist, the edges are neat, opaque, and the center is raised. Gram staining was typically positive, and under the microscope...

Embodiment 2

[0065] Example 2: Cultivation of Lactobacillus fermentum WHH3906 After the second-generation activation of Lactobacillus fermentum WHH3906, the bacterial strain of the present invention was inoculated in MRS medium at a 1% inoculum size, cultivated at 37°C for 24 hours, and sampled once every 1 hour for determination The optical density (OD) value at a wavelength of 600nm, the growth curve was drawn, and three replicates were set.

[0066] The result is as figure 2 As shown, after the lag phase of 0-2h, Lactobacillus fermentum WHH3906 began to grow rapidly and entered the logarithmic growth phase from 2h, and entered the stable phase at the end of the logarithmic phase at 13h.

Embodiment 3

[0067] Embodiment 3: the acid resistance and bile salt resistance performance of Lactobacillus fermentum WHH3906 bacterial strain Lactobacillus fermentum WHH3906 of the present invention, contrast commercial bacterial strain Lactobacillus casei Daita strain (LcS) and contrast commercial bacterial strain Lactobacillus rhamnosus GG (LGG) through second generation activation Finally, take the bacterial solution at the end of logarithmic growth, centrifuge at 4000rpm for 10min, discard the supernatant to obtain the bacterial sludge, and perform the following operations: ① Add the same volume of MRS solution with pH 2.5, mix by pipetting, incubate at 37°C, and use Measure the change of bacterial count at 0 point and after incubation for 1h, 2h, and 4h by dilution coating counting method; Cloth counting method was used to measure the change of bacterial count at 0 point and after incubation for 4h and 8h, and three repetitions were set. Strain survival rate calculation formula:

[...

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Abstract

The invention relates to the field of microorganisms, and discloses lactobacillus fermentum with a weight reducing function and an application of the lactobacillus fermentum. The lactobacillus fermentum is named as WHH3906, is separated from butter collected in Tibet Autonomous Region in China, is preserved in China General Microbiological Culture Collection Center on March 13, 2020, has a preservation number of CGMCC NO.19472, and is classified and named as lactobacillus fermentum. The lactobacillus fermentum strain can effectively reduce serum leptin level to recover leptin sensitivity of anorganism, effectively prevent and treat obesity, relieve non-alcoholic fatty liver and relieve chronic inflammation. After being compounded with lactobacillus plantarum 1701, the lactobacillus fermentum can synergistically relieve chronic inflammation.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a lactobacillus fermentum with weight loss function and its application. Background technique [0002] According to a new study by my country's Center for Disease Control and Prevention, a survey of more than 170,000 people nationwide found that the prevalence of obesity among adults in the country is 14%, among which the prevalence rate of men is 14%, and that of women is 14.1%. ; The prevalence of abdominal obesity was 35.1%, 30.7% for men and 32.4% for women, and there were regional and gender differences. Among them, the obesity and abdominal obesity rates in Beijing, Tianjin and Hebei were the highest. However, with the advent of a well-off life, the living standards of Chinese residents will be improved, and the number of overweight and obese people will increase. A series of subsequent diseases will also bring troubles to people's lives and bring The development of the national eco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A23L2/38A23L2/39A23L19/20A23C9/123A23C17/02A61K35/747A61P3/04A61P1/16A61P29/00A61P3/06C12R1/225
CPCA23L33/135A23L2/382A23L2/39A23L19/20A23C9/1234A23C17/02A61K35/747A61P3/04A61P1/16A61P29/00A61P3/06A23V2002/00A23V2400/143A23V2200/332A23V2200/3204A23V2200/30Y02A50/30
Inventor 孙盛陈苏俞赟霞陈丽娥李言郡余腾斐陈彩玲郑志瑶陈作国葛红娟李理
Owner HANGZHOU WAHAHA TECH
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