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Application of bacillus subtilis HF1 to astragalus membranaceus planting

A technology of Bacillus subtilis and HF-1, applied in the field of microorganisms, can solve the problems of excessive pesticide residues in Astragalus membranaceus, limit the sustainable production and output of Astragalus membranaceus, affect the food safety of Astragalus membranaceus, and achieve the effect of changing community changes

Active Publication Date: 2021-03-05
北京中科金株生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the expansion of the planting area of ​​Astragalus membranaceus, the rampant occurrence of soil-borne diseases mainly caused by Fusarium acuminatum and F.
The continuous cropping obstacles of Astragalus membranaceus are mainly caused by soil-borne pathogenic fungi and autotoxicity. In order to effectively prevent and control diseases, a large number of high-residue chemical pesticides are used in the production of Astragalus membranaceus. Although the yield and appearance quality are guaranteed, the pesticide residues in Astragalus membranaceus exceed the standard , which directly affects the edible safety of Astragalus

Method used

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  • Application of bacillus subtilis HF1 to astragalus membranaceus planting
  • Application of bacillus subtilis HF1 to astragalus membranaceus planting
  • Application of bacillus subtilis HF1 to astragalus membranaceus planting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The present embodiment provides a kind of preparation method of Bacillus subtilis (Bacillus subtilis) HF1 fermented liquid, specifically comprises the steps:

[0032] (1) After activating the Bacillus subtilis HF1 strain on the plate, pick the monoclonal strain, inoculate it into 100 mL of LB medium, and culture it overnight on a shaker at 30° C. and 200 rpm.

[0033] (2) Inoculate the Bacillus subtilis HF1 strain into 2L of LB medium, culture on a shaker at 30° C. and 150 rpm for 36 hours, collect the fermentation broth and store it at room temperature for later use.

Embodiment 2

[0035] The present embodiment provides a kind of application method of Bacillus subtilis (Bacillus subtilis) HF1, specifically as follows:

[0036] 1. Community settings

[0037] Land plots: divided into non-heavy-cropping land and heavy-cropping land, located in;

[0038] Seedling selection: select astragalus seedlings of similar size;

[0039] Planting method: ditching, about 15cm deep, about 2m long, 40cm row spacing, place seedlings in the same direction, place 10 seedlings in each ditch, 5 ditches for each plot, repeat each plot as a treatment, each treatment Repeat 3 times.

[0040] 2. Seedling treatment

[0041] Get the Bacillus subtilis (Bacillus subtilis) HF1 fermented liquid that embodiment 1 provides to carry out experiment in non-replanted land (marked as A) and replanted land (marked as B); Before covering soil, fermented liquid is diluted 100 times and 300 times respectively with clear water times, use a sprayer to evenly spray the diluted solution on the soi...

Embodiment 3

[0043] In this example, the control effect of Bacillus subtilis HF1 grown in Example 2 and the content of effective components of Astragalus membranaceus were tested.

[0044] 1. Disease classification and disease index processing standards:

[0045] According to the degree of damage of Astragalus root disease, this experiment divides the disease into 5 levels, as follows:

[0046] Grade 0, no lesion;

[0047] Grade 1, no large blackhead lesions at the junction of rhizomes, and less than 10 lesions in other places;

[0048]Grade 3, no large blackhead lesions at the junction of rhizomes, and more than 10 lesions in other places;

[0049] Grade 5, there are large blackhead lesions at the junction of rhizomes, and the number of lesions in other places is less than 10;

[0050] Grade 7, there are large blackhead lesions at the junction of rhizomes, and the number of lesions in other places is more than 10.

[0051] Disease index = ∑ (number of diseased plants at all levels × v...

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Abstract

The invention relates to application of bacillus subtilis HF1 to astragalus membranaceus planting. In the application, the bacillus subtilis HF1 can effectively increase the content of calycosin-7-glucoside and astragaloside in astragalus membranaceus; and particularly in a continuous cropping land, the content of the calycosin-7-glucoside is increased by 35.14%-63.51%, and the content of the astragaloside is increased by 26.39%-45.83%. Meanwhile, the bacillus subtilis HF1 disclosed by the invention can reduce the occurrence of soil-borne diseases of the astragalus membranaceus, the control effect in a non-continuous cropping land can reach 57%-60%, and the control effect in a continuous cropping land can reach 54%-56%.

Description

technical field [0001] The invention relates to the field of microbes, in particular to the application of Bacillus subtilis HF1 in planting Astragalus membranaceus. Background technique [0002] Astragalus membranaceus is a perennial herbaceous plant of leguminous family. Its roots are used as medicine, and the roots are mostly lignified. It is a traditional Chinese medicinal material with high medicinal value. With the expansion of the planting area of ​​Astragalus, the rampant occurrence of soil-borne diseases mainly caused by Fusarium acuminatum and F.solani has limited the sustainable production and increase of the yield of Astragalus. The continuous cropping obstacles of Astragalus membranaceus are mainly caused by soil-borne pathogenic fungi and autotoxicity. In order to effectively prevent and control diseases, a large number of high-residue chemical pesticides are used in the production of Astragalus membranaceus. Although the yield and appearance quality are guaran...

Claims

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Application Information

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IPC IPC(8): A01G22/25A01G22/40A01G7/06A01G13/00C12N1/20C12R1/125
CPCA01G22/25A01G22/40A01G7/06A01G13/00C12N1/20
Inventor 胡赞民范成明陈宇红李旻辉王文乐侯兴坤傅洪波李将健何红军
Owner 北京中科金株生物科技有限公司
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