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Application of inhibiting proliferation and growth of non-small cell lung cancer by preventing LACTB2 expression

A non-small cell lung cancer, cell technology, applied in the field of genetic engineering, to achieve the effect of reducing value-added ability and exercise ability

Active Publication Date: 2021-02-26
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, LACTB2 has not been reported in lung cancer cells, so an application to inhibit the expression of LACTB2 to inhibit the proliferation and growth of non-small cell lung cancer was designed

Method used

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  • Application of inhibiting proliferation and growth of non-small cell lung cancer by preventing LACTB2 expression
  • Application of inhibiting proliferation and growth of non-small cell lung cancer by preventing LACTB2 expression
  • Application of inhibiting proliferation and growth of non-small cell lung cancer by preventing LACTB2 expression

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Embodiment 1

[0052] see Figure 1-Figure 14 , the present invention provides a technical solution: an application of preventing the expression of LACTB2 to inhibit the proliferation and growth of non-small cell lung cancer.

[0053] In this embodiment, preferably, include:

[0054] Construction of S1. LACTB2 overexpression plasmid;

[0055] S2. Construction of lung cancer cell lines overexpressing LACTB2;

[0056] Construction of S3.LACYB2 knockdown vector;

[0057] Construction of S4. LACTB2 knockdown lung cancer cell lines;

[0058] S5. Detecting the effect of LACTB2 on the non-anchor-dependent growth of non-small cell lung cancer;

[0059] S6. Detecting the effect of LACTB2 on the proliferation ability of non-small cell lung cancer;

[0060] S7. Detecting the effect of LACTB2 on the exercise capacity of non-small cell lung cancer;

[0061] S8. detecting the effect of LACTB2 on the regulation of E-cadherin expression;

[0062] S9. Detection of the effect of LACYB2 on the morpholog...

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Abstract

The invention discloses application of inhibiting proliferation and growth of non-small cell lung cancer by preventing LACTB2 expression. According to the application, the LACTB2 is knocked down to prepare lentivirus, and the lentivirus is transfected into lung cancer cells; the situation that knockdown of the LACTB2 reduces the non-anchoring dependent growth ability of non-small cell lung cancercells, reduces the proliferation ability of the lung cancer cells, reduces the movement ability of the non-small cell lung cancer cells, influences the functions of the lung cancer cells by regulatingand controlling the expression of E-Cadherin, and can maintain no obvious change of pseudopodia in cytoskeletons is detected; and therefore, the LACTB2 is conveniently knocked down to inhibit the proliferation of the non-small cell lung cancer cells, and a new clinical treatment target and a treatment direction are provided for the treatment of tumors.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to an application of preventing the expression of LACTB2 to inhibit the proliferation and growth of non-small cell lung cancer. Background technique [0002] In the world, although the treatment of cancer is increasing, the death caused by cancer is still the biggest difficulty in medical treatment. According to statistics, in 2018, almost half of the world's cancer-related deaths occurred in Asia. Lung cancer is one of the most prevalent cancers, accounting for 11.6% of all new cancer patients. It is the main cause of death caused by cancer, accounting for about 20% of cancer deaths. Not only in my country but also in the world, lung cancer It is still an important factor in the death of cancer patients, and it is also the biggest difficulty in cancer treatment in my country. The national cancer prevalence rate and mortality rate are among the top. According...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/867C12N15/66C12N5/10C12Q1/686C12Q1/02G01N33/68
CPCC12N15/113C12N15/86C12N15/66C12Q1/686G01N33/68G01N33/5029C12N2310/14C12N2740/15043C12Q2521/107C12Q2545/101
Inventor 李勇李东佳王云陈荣
Owner ANHUI UNIVERSITY
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