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Application of yak milk fermentation extract

A technology of yak milk and extract, which is applied in the application field of fermented extract of yak milk, can solve the problems of repairing aging skin without microecology, achieve good application prospects, reduce the ratio, and promote the effect of proliferation

Pending Publication Date: 2021-02-26
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] At present, there is no report on the use of fermented yak milk extracts as external skin agents, and there is no report on the use of yak milk fermented extracts to improve skin surface microecology or repair aging skin, and to inhibit tyrosinase. Active as a whitening active ingredient reported

Method used

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  • Application of yak milk fermentation extract
  • Application of yak milk fermentation extract
  • Application of yak milk fermentation extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation of yak milk fermentation product filtrate

[0034] The base material of the fermentation product is yak milk or dry milk powder of yak milk, which is produced in Pan-Himalayan region. Yak milk is mixed with deionized water, and the content of yak milk solids in the fermentation medium / culture solution is 4wt%; after high temperature and high pressure sterilization, Lactobacillus plantarum ( Lactobacillus plantarum ) to ferment for 36 hours at 35-40°C and 150-250rpm.

[0035] Preparation of yak milk fermentation filtrate:

[0036] (1) Centrifuge the fermentation product at 4-10°C and 6000rpm for 10-30min, and take the supernatant;

[0037] (2) Adjust the pH to 5.0-7.0, bathe in water at 80-90°C for 30-60 minutes, centrifuge at 1000-4000 rpm for 10-30 minutes, and take the supernatant;

[0038] (3) Choose a filter membrane with a pore size of 0.05, 0.10 or 0.22 μm, and pre-soak the filter membrane in 75% (v / v) ethanol; clean the filter filter and...

Embodiment 2

[0042] Example 2 Effect of yak milk fermented extract on surface flora

[0043] (1) Experimental materials

[0044] 1. Experimental strains

[0045] Staphylococcus epidermidis S. e ( Staphylococcus epidermidis ), Staphylococcus aureus S. a ( Staphylococcus aureus ).

[0046] 2. Medium

[0047] LB medium: 0.5% powder, 1.0% tryptone, 1.0% NaCl, the balance is water, and the percentages are mass percentages. 121 ℃, 20min high temperature and high pressure sterilization, and cool down for later use.

[0048] 3. Sample information

[0049] Blank sample (Control): Sterile physiological saline: 0.9% NaCl, the balance is water, and the stated percentages are mass percentages. 121 ℃, 20min high temperature and high pressure sterilization, and cool down for later use.

[0050] Experimental sample: the yak milk fermentation product filtrate obtained in Example 1, wherein the solid content is 1.5wt%.

[0051] (2) Experimental steps

[0052] 1. to deposit S. a and S. e ...

Embodiment 3

[0057] Example 3 Cell proliferation of yak milk fermentation product

[0058] (1) Experimental materials

[0059] Cells: dermal fibroblast Fibroblast, human immortalized epidermal HaCaT.

[0060] Experimental sample: yak milk fermentation product filtrate was prepared with deionized water to make solutions of different concentrations, and various concentrations of yak milk fermentation product filtrate were added to the serum-free DMEM or MEM medium required for different cell growth to ferment yak milk in the medium The dry matter content of the product filtrate is 0.015%, 0.075% and 0.15%, and the percentages are mass percentages.

[0061] (2) Experimental method

[0062] The yak milk fermentation product filtrate was prepared with deionized water to make different concentrations of solutions, added to the human fibroblast culture medium, and deionized water without samples was used as a blank control. After 48 hours of cultivation, the cells were stained with the MTT meth...

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Abstract

The invention belongs to the fields of natural extracts and daily chemicals, and particularly relates to application of a yak milk fermentation extract. The yak milk fermentation extract is obtained by fermenting yak milk with lactobacillus plantarum and carrying out centrifugal filtration, so that the yak milk fermentation extract is reduced in staphylococcus aureus to staphylococcus epidermidisratio, and capable of promoting proliferation of dermal fibroblasts and epidermal cells so as to realize the effects of regulating skin flora, maintaining and / or repairing skin surface micro-ecology,repairing aged skin and whitening the skin; and thus, the yak milk fermentation extract can be used for preparing a topical agent for skin use, as well as an active ingredient for repairing the aged skin, whitening the skin and improving the skin surface micro-ecology.

Description

technical field [0001] The invention belongs to the field of plant extraction and daily chemical industry, and specifically relates to the application of yak milk fermented extract. Background technique [0002] It is not uncommon for humans to use milk and dairy products for beauty and skin care. For example, the ancient Romans believed that bread soaked in milk can make the skin white, tender and smooth. Milk protein and fat in milk can be attached to the skin surface, which can moisturize the skin; enzymes in milk can promote the decomposition of cutin on the surface of the skin. Yak milk contains high content of vitamins, proteins, amino acids and trace elements. Compared with milk, its nutrients are more abundant and higher in content, such as amino acid and vitamin A content are 15% and 6% higher than milk respectively; calcium content is 1.13 times that of ordinary milk, and zinc content is 3 times that of milk. It has been reported that people can use casein in dai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/20A61Q19/00A61Q19/08A61Q17/00C12P1/02C12R1/865
CPCA61K35/20A61Q19/00A61Q19/08A61Q17/005C12P1/02A61K2800/85C12R2001/865C12N1/185
Inventor 李俊章漳黄俊
Owner JALA GROUP CORPORATION
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