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Preparation and use method of culture medium suitable for morchella esculenta breeding

A culture medium and morel technology, which is applied in the fields of bioengineering and biological planting and cultivation, can solve the problems of many miscellaneous bacteria, low survival rate of breeding, and low yield of morel, so as to increase the amount of bacteria and increase the unit of bacteria Quantity, cost reduction effect

Pending Publication Date: 2021-02-26
安徽乐永园农业科技有限公司
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Problems solved by technology

[0003] At this stage, because the economic value of morels is large and suitable for promotion, many farmers have begun to plant morels. However, the selection of morels is very important, and in the breeding of morels, morel breeding The yield of cultivation has not been high, which restricts the artificial planting of morels, and the low survival rate of breeding and the presence of many miscellaneous bacteria have always existed, which has also become a condition that restricts the promotion of morel planting technology

Method used

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  • Preparation and use method of culture medium suitable for morchella esculenta breeding
  • Preparation and use method of culture medium suitable for morchella esculenta breeding
  • Preparation and use method of culture medium suitable for morchella esculenta breeding

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preparation example Construction

[0023] The preparation process of the culture medium comprises the following steps:

[0024] A. Get several grams of poplar roots, wash and crush them, pour the pulverized poplar roots into water, boil, keep for a period of time, cool, and filter the poplar roots, and the obtained soup is poplar root additive;

[0025] B. Take water, crush raw potatoes, put them in water, add agar strips, boil and simmer for a while, until the agar strips are completely melted, and the potatoes are cooked until greasy but not rotten, add glucose, stir, and dissolve completely Afterwards, the intermediate is obtained, cooled for subsequent use;

[0026] C. get the poplar root additive prepared in step A, the intermediate prepared in step B, mix in proportion, fully boil, and fully stir and mix to obtain the culture medium;

[0027] D. Divide the culture medium prepared in step C into clean test tubes, seal them with sterile cotton, and then put them in an autoclave for autoclaving. After a per...

Embodiment 1

[0035] A method for preparing and using a culture medium suitable for hickory chick culture, including the preparation of the culture medium, the culture medium includes raw potatoes, glucose, agar, and poplar root additives, calculated by weight components, including poplar root additives 5%, raw potatoes 25%, glucose 15%, agar 25%, and the rest is water. (70)

Embodiment 2

[0037] A method for preparing and using a culture medium suitable for hickory chick culture, including the preparation of the culture medium, the culture medium includes raw potatoes, glucose, agar, and poplar root additives, calculated by weight components, including poplar root additives 10%, raw potatoes 25%, glucose 15%, agar 25%, and the rest is water. (75)

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Abstract

The invention discloses a preparation and use method of a culture medium suitable for morchella esculenta breeding. The method comprises the following step of preparing a fungus breeding culture medium which comprises the following components in percentage by weight of 5%-15% of poplar root additive, 25%-40% of raw potatoes, 10%-15% of glucose, 20%-30% of agar and the balance of water. The culturemedium is adopted to better breed and cultivate fungi, especially for morchella esculenta strains, has the advantages of being easy to culture, fast in growth and good in bacterial colony, has the advantages of being low in cost and wide in material source, can perform breeding according to morchella esculenta habits in a targeted manner, and is better in bacterial colony, more developed in hyphaand more beneficial to next-step transplantation.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, specifically the technical field of biological planting and cultivation, especially for the cultivation and breeding of hickory chick, and involves the preparation of medium and its use method. Background technique [0002] Morel is a precious edible fungus and medicinal fungus. Morel contains 20% crude protein, 26% crude fat, 38.1% carbohydrate, and also contains a variety of amino acids and vitamins, especially glutamic acid content as high as 1.76 %. Modern medical clinical research shows that Morchella has the effects of improving immunity, anti-tumor, and anti-aging, and has high application value. [0003] At this stage, because the economic value of morels is large and suitable for promotion, many farmers have begun to plant morels. However, the selection of morels is very important, and in the breeding of morels, morel breeding The output of cultivating is not high all the time, ...

Claims

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Application Information

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IPC IPC(8): A01G18/20A01G18/40C12N1/14C12N1/38C12R1/645
CPCA01G18/20A01G18/40C12N1/14C12N1/38
Inventor 郭永乐
Owner 安徽乐永园农业科技有限公司
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