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Establishment method of oyster powder fingerprint spectrum and fingerprint spectrum

A technique of fingerprinting and establishing methods, which is applied in the field of quality identification of traditional Chinese medicine, can solve the problems of no oyster powder fingerprint research, complex components of oyster powder, and qualitative and quantitative analysis that cannot fully reflect the comprehensive information of the compound, and achieve large peak areas and reproducible results. Good performance and moderate retention time

Active Publication Date: 2021-02-23
QINGDAO NAT LAB FOR MARINE SCI & TECH DEV CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the composition of oyster powder is complex, and the qualitative and quantitative analysis of one or two components is difficult to fully reflect the comprehensive information of the compound, and so far there is no related research on the fingerprint of oyster powder

Method used

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  • Establishment method of oyster powder fingerprint spectrum and fingerprint spectrum
  • Establishment method of oyster powder fingerprint spectrum and fingerprint spectrum
  • Establishment method of oyster powder fingerprint spectrum and fingerprint spectrum

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Experimental program
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Effect test

preparation example Construction

[0036] 2. Preparation of oyster powder

[0037] A total of 15 batches of prescription decoction pieces were purchased from major decoction piece companies across the country, and the origin information is shown in Table 1-Table 4.

[0038] Table 1. Sources of Astragalus decoction pieces

[0039]

[0040] Table 2. Sources of ephedra root decoction pieces

[0041]

[0042] Table 3. Sources of floating wheat decoction pieces

[0043]

[0044] Table 4. Sources of ephedra root decoction pieces

[0045] herbs Numbering place of origin Manufacturer Roasted Oysters 1 liaoning Anhui Wansheng Chinese Herbal Pieces Co., Ltd.

[0046] The preparation method of oyster powder of the present invention is as follows: take 3 parts of calcined oyster, 3 parts of astragalus root, 3 parts of ephedra root and 2.5 parts of floating wheat, the above four flavors, soak in water for 1 hour, decoct twice, each time for 2 hours , add water 8 times the weight of...

Embodiment 1

[0050] The establishment method of oyster scattered fingerprint collection described in embodiment 1, comprises the following steps:

[0051] Step 1: Take the reference substances of actecoside, formononetin, and actecoisoflavone, and add methanol to prepare the concentrations of 0.22, 0.31, and 0.28 mg·mL respectively −1 mixed reference solution.

[0052] Step 2: Take 15 batches of oyster powder test samples (batch number 20200801-20200815), take about 50mg, accurately weigh, add 2mL water to dissolve; Cleanert S C18 solid phase extraction column treatment, the treatment process is: methanol activation, Equilibrate with water, load the sample, wash with 2 times the column volume of aqueous solution, elute with 2 times the column volume of 0.4% formic acid methanol, collect the eluate, N 2 Blow dry, add 200 to the residue mu L methanol was redissolved, centrifuged, and the supernatant was taken to prepare 15 batches of oyster powder for the test solution.

[0053] Step 3: P...

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Abstract

The invention discloses an establishment method of an oyster powder fingerprint spectrum and the fingerprint spectrum. The establishment method of the fingerprint spectrum comprises the following steps of: 1, preparing a reference peak solution; 2, preparing an oyster powder test solution; 3, precisely sucking a certain amount of the reference peak solution and the test solution respectively, injecting the solutions into a liquid chromatograph, and recording a chromatogram; and 4, exporting the oyster powder fingerprint spectrum obtained in the step 3 from the instrument, importing the oysterpowder fingerprint spectrum into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, selecting chromatographic peaks existing in chromatograms of different batchesof oyster powder test samples as common peaks, generating a control fingerprint spectrum of the oyster powder by using an average value calculation method, and calculating the relative retention timeand the relative peak area of each common peak. The method for establishing a fingerprint spectrum has the advantages of simplicity, convenience, stability, high precision, good reproducibility and the like. The oyster powder fingerprint spectrum provided by the invention can comprehensively and objectively characterize the quality of oyster powder, and is beneficial to comprehensive monitoring of the quality of medicines.

Description

technical field [0001] The invention relates to a method for establishing an oyster loose fingerprint and the oyster loose fingerprint established by the method, belonging to the technical field of traditional Chinese medicine quality identification. Background technique [0002] Fingerprint of traditional Chinese medicine is an effective means of comprehensive analysis to identify the authenticity of traditional Chinese medicinal materials or Chinese patent medicines by using modern analysis technology, and to evaluate the consistency and stability of the quality of raw materials, semi-finished products and finished products. Compared with the identification of existing single active ingredients or index ingredients, the fingerprint technology of traditional Chinese medicine integrates various effective chemical information to make the results more scientific and reasonable. At present, the fingerprint of traditional Chinese medicine has become an important method of qualit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8686G01N30/8634
Inventor 马晓青魏宝红胡淑曼刘红兵管华诗
Owner QINGDAO NAT LAB FOR MARINE SCI & TECH DEV CENT
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