Kit for homologous recombination repair defect detection

A detection kit and defect detection technology, applied in the fields of molecular biology and medicine, can solve the problems of lack of DNA homologous recombination repair, lack of wild genotype, cancer occurrence, etc., and achieve the effect of simple structure, low cost and convenient use

Inactive Publication Date: 2021-02-23
宁波爱她基因科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, cells with BRCA1 or BRCA2 mutations lack the wild genotype, resulting in the loss of DNA homologous recombination repair, resulting in gene mutations, leading to cancer

Method used

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  • Kit for homologous recombination repair defect detection
  • Kit for homologous recombination repair defect detection
  • Kit for homologous recombination repair defect detection

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Embodiment Construction

[0014] The technical solutions in the embodiments of the present invention will be clearly and completely described below, obviously, the described embodiments are only some of the embodiments, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0015] A kit for homologous recombination repair defect detection, comprising a library construction kit, a hybridization kit, a probe kit and an adapter kit.

[0016] The kit for building a library comprises a packaging box, a liner of the kit for building a library, and a reagent tube. Including 1 tube of end repair reaction solution 300 μl, 1 tube of ligation reaction solution 500 μl, 1 tube of ligase 100 μl, 1 tube of amplification reaction solution 1000 μl, and 1 tube of amplification primer 200 μl.

[0017] The hybridization kit comprises a pa...

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Abstract

The invention discloses a kit for homologous recombination repair defect detection. A multi-probe targeted capture technology is combined with a next-generation sequencing technology to enrich and detect the variation conditions of 25 genes, wherein the 25 genes include ARID1A, BRCA1, BRCA2, POLG, ATR, BRIP1, MSH2, RAD51C, ATM, MLH1, MRE11A, RAD50, ATRX, CHECK1, MUTYH, RAD51D, BAP1, CHECK2, NBN, SMARCB1, BARD1, FANCE, PALB2, WRN and BLM.

Description

technical field [0001] The invention relates to the fields of molecular biology and medicine, in particular to a kit for detecting recombinant repair defects. Background technique [0002] The development of cancer requires a series of lesion stages. Its development process is dominated by gene mutations, including DNA, RNA, protein and other levels of lesions. Among them, DNA changes are an important factor in the occurrence of cancer, including single nucleotide mutations. , small insertions and deletions, large structural variations and other common forms of variation. With the deepening of cancer research, the importance of homologous recombination loss (HRD) mechanism in different cancers has been continuously discovered. More than 50% of patients with high-grade serous ovarian cancer have significant DNA repair gene deletions, and 20-25% of prostate cancers have inactivating mutations in BRCA1 / BRCA2 or other genes involved in the homologous recombination pathway. Dur...

Claims

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Application Information

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IPC IPC(8): C12Q1/6874
CPCC12Q1/6874
Inventor 刘赟
Owner 宁波爱她基因科技有限公司
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