Hybrid rice anther culture method

A cultivation method, hybrid rice technology, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of low seedling resistance, low seedling emergence rate, low anther callus induction rate, etc., to improve induction rate and emergence rate, promoting application, and increasing resistance

Pending Publication Date: 2021-02-23
黑龙江省农业科学院绥化分院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the main problems of rice anther culture are low anther callus induction rate, low seedling resistance, and low seedling emergence rate, which seriously hinder the application of anther culture technology in the hybrid breeding practice of common wild rice and cultivated rice

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for cultivating hybrid rice anthers. The culturing method includes collection and pretreatment of anthers, disinfection and sterilization of anthers, culture of anther callus, rooting cultivation of differentiated seedlings of anthers, hardening of anthers and transplanting.

[0022] The collection and pretreatment of described anther: select field to collect materials, get young ear before six o'clock in the morning of a sunny day, choose the rice ear that distance is 6-7cm between flag leaf ring and next leaf ring, ear is not broken, keep Put the two leaves and leaf sheaths into a bucket filled with clear water immediately after picking the ears to avoid dehydration of the ears, and then as an antifreeze exercise, add ice cubes to the water bucket and control the water temperature at 2-3°C.

[0023] Disinfection and sterilization of the anthers: in the laboratory, put the blade and the leaf sheath into 70% ethanol to soak for 1 minute, let it stand for ten min...

Embodiment 2

[0032] The difference between this embodiment and embodiment 1 is that it has not undergone antifreeze exercise.

[0033] A method for cultivating hybrid rice anthers. The culturing method includes collection and pretreatment of anthers, disinfection and sterilization of anthers, culture of anther callus, rooting cultivation of differentiated seedlings of anthers, hardening of anthers and transplanting.

[0034] The collection and pretreatment of described anther: select field to collect materials, get young ear before six o'clock in the morning of a sunny day, choose the rice ear that distance is 6-7cm between flag leaf ring and next leaf ring, ear is not broken, keep Two leaves and leaf sheaths are put into a bucket filled with clear water immediately after the ears are taken to avoid water loss from the ears.

[0035] Disinfection and sterilization of the anthers: in the laboratory, put the blade and the leaf sheath into 70% ethanol to soak for 1 minute, let it stand for te...

Embodiment 3

[0044] The difference between this embodiment and embodiment 1 is that it is not sterilized by ultraviolet rays.

[0045] A method for cultivating hybrid rice anthers. The culturing method includes collection and pretreatment of anthers, disinfection and sterilization of anthers, culture of anther callus, rooting cultivation of differentiated seedlings of anthers, hardening of anthers and transplanting.

[0046] The collection and pretreatment of described anther: select field to collect materials, get young ear before six o'clock in the morning of a sunny day, choose the rice ear that distance is 6-7cm between flag leaf ring and next leaf ring, ear is not broken, keep Put the two leaves and leaf sheaths into a bucket filled with clear water immediately after picking the ears to avoid dehydration of the ears, and then as an antifreeze exercise, add ice cubes to the water bucket and control the water temperature at 2-3°C.

[0047] Disinfection and sterilization of the anthers: ...

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PUM

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Abstract

The invention discloses a hybrid rice anther culture method. The hybrid rice anther culture method comprises the steps of anther collection and pretreatment, anther disinfection and sterilization, anther callus culture, anther differentiation seedling rooting culture, anther seedling hardening and transplantation. According to the hybrid rice anther culture method, the rice anther is pretreated, and resistant callus induction culture and improved culture medium proliferation culture methods are adopted, so that the inductivity and the emergence rate are improved and the resistance of seedlingsis increased during rice anther culture, and the main problems of low anther callus inductivity, low seedling resistance and low emergence rate of conventional rice anther culture are solved. The application of an anther culture technology to crossbreeding practice of common wild rice and cultivated rice is greatly promoted.

Description

technical field [0001] The invention relates to a rice cultivation method, in particular to a hybrid rice anther cultivation method. Background technique [0002] Rice anther culture is to inoculate anthers that have developed to a certain stage on an artificially configured medium through aseptic techniques, and induce their pollen (microspores) to form callus, and then differentiate into complete plants. During the culture process, after the chromosomes are doubled naturally or artificially, they become homozygous diploids. The homozygous diploids produced by this chromosome doubling are very stable genetically and do not segregate traits. [0003] The use of rice anther culture in breeding is reflected in the early and stable offspring, rapid acquisition of pure lines, shortening the breeding period, and improving selection efficiency. The progeny of conventional hybridization needs to be above the F5 generation, and the recrossing combination requires more algebraic tra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 谢树鹏刘立超门龙楠魏中华孙中华宗天鹏张广彬景玉良刘宝海聂守军高世伟刘晴刘宇强马成常汇琳符强冯鑫张起昌米刚
Owner 黑龙江省农业科学院绥化分院
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