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Screening method and application of ERO1alpha molecular marker related to liver injury endoplasmic reticulum stress unfolded protein response

A technology of endoplasmic reticulum stress and molecular markers, applied in biochemical equipment and methods, microbial measurement/testing, analysis materials, etc., to achieve the effect of easy promotion

Inactive Publication Date: 2021-02-19
NINGXIA MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether ERO1α mediates the mechanism of HHcy-induced liver injury is unknown

Method used

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  • Screening method and application of ERO1alpha molecular marker related to liver injury endoplasmic reticulum stress unfolded protein response
  • Screening method and application of ERO1alpha molecular marker related to liver injury endoplasmic reticulum stress unfolded protein response
  • Screening method and application of ERO1alpha molecular marker related to liver injury endoplasmic reticulum stress unfolded protein response

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Experimental program
Comparison scheme
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Embodiment Construction

[0030] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0031] 1 material

[0032] 1.1 Main reagents

[0033] Fluorescence quantitative kit from Fermentas, USA, Thermo Fisher reverse transcription kit; Beibo cell apoptosis detection kit; GRP78, PERK, ATF6, and XBP-1 antibodies.

[0034] 1.2 Main instruments

[0035] Ultra-clean workbench, Bio-Rad film transfer apparatus, Bio-Rad electrotransfer apparatus, nucleic acid analyzer, gel imaging system, low-temperature refrigerator, mini centrifuge, high-speed low-temperature centrifuge, cryostat, fluorescent quantitative PCR and ordinary PCR.

[0036] 2 Experimental methods

[0037] 2.1 Selection of experimental subjects

[0038] CBS + / - The mice were purchased from the Jackson Laboratory of the United States, raised in an SPF environment in the Experimental Animal Center of Ningxia Medical University, and randomly divided into the control grou...

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Abstract

The invention discloses a screening method and application of an ERO1alpha molecular marker related to liver injury endoplasmic reticulum stress unfolded protein response. CBS<+ / ->gene knockout mice and liver cells are taken as research objects, RNA and protein of tissues or cells are extracted, genes related to HHcy induced liver injury are screened out, the expression change of ERO1alpha and UPRrelated proteins is verified at the cellular level, and the Hcy-induced liver injury specific disease combined diagnosis method composed of ERO1alpha and UPR protein gene networks is established. Themethod is simple and reliable, materials are convenient to obtain, the sensitivity and specificity are highly matched with those of biochemical diagnosis results, and the method has great applicationvalue in early diagnosis and prognosis evaluation of HHcy induced liver injury diseases.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a screening method of molecular markers of ERO1α related to liver injury endoplasmic reticulum stress unfolded protein response and application thereof. Background technique [0002] Homocysteine ​​(Hcy) is a thiol-containing non-protein amino acid produced during the nucleic acid methylation and demethylation of methionine. The concentration of Hcy in plasma exceeds 15 μmol / l plasma is defined as hyperhomocysteine Acidemia (HHcy). Involved in the pathogenesis of various diseases, including hypertension and coronary artery disease. Hcy is metabolized in the body mainly through two important ways: remethylation and transsulfuration. The transsulfuration pathway plays an important role in the process of liver damage caused by Hcy, and the process is mainly regulated by cystathionase ether synthase (CBS ) regulation, when the synthesis of CBS decreases, Hcy cannot be fully...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/573G01N23/04
CPCC12Q1/6883G01N33/573G01N23/04C12Q2600/158C12Q2600/178C12Q2600/136G01N2800/085G01N2333/90212G01N2223/03G01N2223/6126
Inventor 姜怡邓张慧萍吴欣妍焦运徐龙张辉刘圆马芳
Owner NINGXIA MEDICAL UNIV
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