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DNA bar code for identifying lilium plants and application thereof

A barcode, Lilium technology, applied in the field of molecular biology, achieves good stability, high repeatability, and accurate identification

Inactive Publication Date: 2021-02-05
BEIJING AGRO BIOTECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to provide a DNA barcode that can quickly and easily identify and distinguish Lilium resources, so as to solve and improve the classification of wild species of Lilium plants.

Method used

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  • DNA bar code for identifying lilium plants and application thereof
  • DNA bar code for identifying lilium plants and application thereof
  • DNA bar code for identifying lilium plants and application thereof

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Embodiment Construction

[0035] A DNA barcode for identification of Lilium plants, spliced ​​from three gene fragments trnP-psaJ-rpI33, psbB-psbH and ITS, used to obtain primers for DNA barcodes, including: used to amplify trnP-psaJ- The nucleotide sequence of the primer pair of rpI33 is as shown in SEQ ID NO:1 and SEQ ID NO:2 in the sequence listing, and the nucleotide sequence of the primer pair for amplifying psbB-psbH is as SEQ ID NO in the sequence listing: 3 and SEQ ID NO: 4, the nucleotide sequence of the primer pair for amplifying ITS is shown in SEQ ID NO: 5 and SEQ ID NO: 6 in the sequence listing.

[0036] Application of the DNA barcode of the present invention in identifying plants of the genus Lilium.

[0037] The method for identifying plants of the genus Lilium using the DNA barcode of the present invention comprises the following steps:

[0038] (1) Extract the DNA of unknown wild lily plants; the samples to be tested include fresh or dry samples of leaves, scales, and petals of Liliu...

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Abstract

The invention discloses a DNA bar code for identifying lilium plants. The DNA bar code is formed by splicing three gene segments, namely trnP-psaJ-rpI33, psbB-psbH and ITS. Nucleotide sequences of a primer pair for amplifying trnP-psaJ-rpI33 are shown as SEQ ID NO: 1 and SEQ ID NO: 2 in SEQUENCE LISTING, nucleotide sequences of a primer pair for amplifying psbB-psbH are shown as SEQ ID NO: 3 and SEQ ID NO: 4, and nucleotide sequences of a primer pair for amplifying ITS are shown as SEQ ID NO: 5 and SEQ ID NO: 6. The DNA bar code can identify and distinguish lilium resources quickly and easily,and solve and improve classification of wild species of lilium plants.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a DNA barcode for lily plant identification and its application. Background technique [0002] Lilium (Lilium): Belonging to Liliaceae (Liliaceae), the Lily family (Lilieae) is a perennial herb with bulbs and fleshy scales; stems with leaves, not branched, large flowers, solitary or in racemes. China is the origin of lily, and it is also mostly distributed in temperate regions of the northern hemisphere such as eastern Asia, Europe, and North America. Due to the beautiful posture of lilies, there has been a long history of breeding from wild lilies to artificially cultivated varieties. Today, lily is favored by more and more people because of its beautiful flower posture, rich color, beautiful flower meaning and unique flower fragrance. At the same time, it is also widely used in the world's flower market. It is a must for potted plants, landscaping and garden decoration. An in...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/11C12Q1/6895C12Q1/6858C12Q1/6809
CPCC12Q1/6895C12Q1/6858C12Q1/6809C12Q2600/156C12Q2537/165C12Q2531/113C12Q2565/125C12Q2535/122
Inventor 杜运鹏张秀海刘一心张铭芳陈绪清吴健杨凤萍薛静高俊莲陈曦
Owner BEIJING AGRO BIOTECH RES CENT
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