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Preparation method of novel biocontrol microbial agent for kiwi fruit leaf blight and application of preparation method

A biocontrol agent and kiwifruit leaf technology, which is applied in the field of preparation of new biocontrol agents for kiwifruit leaf blight, can solve the problems of large research potential, narrow application scope, backward research and application, etc. The effect is obvious and the effect of improving economic efficiency

Inactive Publication Date: 2021-02-05
陕西省生物农业研究所
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Problems solved by technology

[0007] At present, the prevention and control methods of kiwifruit diseases in China are mainly limited to agricultural control and chemical control, and the research and application in the field of biological control are still relatively backward, especially the application range of antagonistic antibiotic products prepared mainly from antagonistic strains is still relatively narrow. great research potential

Method used

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Embodiment

[0042] This embodiment relates to a preparation method of a novel biocontrol agent for kiwifruit leaf blight, said method comprising the following steps:

[0043] Step 1, the selection of bacterial strain;

[0044] Step 2, the cultivation of bacterial strain;

[0045] Step 3, optimization of strain fermentation conditions;

[0046] Step 4, preparation of compound biocontrol agent.

[0047] Preferably, in Step 1, the strains are selected: Bacillus subtilis Q14, Bacillus amyloliquefaciens J, and Brevibacillus NF2, wherein the preservation number of NF2 is CGMCC No.12749.

[0048] Preferably, in step 2, the cultivation of the bacterial strain is specifically the following steps:

[0049] (1) Strain activation: the original strains stored at -80°C were inoculated on beef extract peptone medium for activation through aseptic manipulation;

[0050] (2) Antagonism test of biocontrol bacteria: plate confrontation method, antagonism test on Brevibacillus NF2, Bacillus subtilis Q14,...

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Abstract

The invention provides a preparation method of a novel biocontrol microbial agent for kiwi fruit leaf blight. The method comprises the following steps of selecting a bacterial strain; culturing the strain; optimizing strain fermentation conditions; and preparing a composite biocontrol microbial agent. According to the method, an antagonistic microbial agent is produced by fully utilizing a modernfermentation engineering technology to replace chemical drugs, biological prevention and treatment of field diseases are performed on the kiwi leaf blight, the growth capacity of crops and the diseaseresistance to plant diseases and insect pests are enhanced, the existing ecological environment is improved, a green and sustainable development mode is established, and a comprehensive long-acting prevention and control system is formed, which will be the main trend of healthy and orderly development of the kiwi fruit industry in the future.

Description

technical field [0001] The invention relates to the field of antibacterial agents; in particular, it relates to a preparation method and application of a novel biocontrol agent for kiwifruit leaf blight. Background technique [0002] Shaanxi is the largest kiwifruit production base in my country. The main planting areas are concentrated in Zhouzhi County, Hu County, Chang'an County, Baqiao District of Xi'an and Mei County of Baoji City. Among them, the planting area of ​​Zhouzhi County alone has reached 130,000 mu. Since 1993, our province has started to build large-scale production bases and standardized management demonstration areas. Up to now, the province’s kiwifruit planting area has exceeded 500,000 hectares, with an output of more than 500,000 tons, accounting for 1 / 3 of the country’s total area and 2% of its output. / 3 or above, ranking first in the country. Kiwifruit products have been exported to more than 10 countries and regions such as Southeast Asia and Russi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01G13/00C12R1/07C12R1/125C12R1/08
CPCC12N1/20A01N63/00A01G13/00
Inventor 付博任平王家哲张锋李英梅陈志杰
Owner 陕西省生物农业研究所
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