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A method for separating and preparing petunienin-3-o-(6-o-p-coumaroyl) glucoside

A technology for petunidin and glucoside, which is applied in the field of separation and preparation of petunidin-3-O-glucoside, can solve the problem that there is no acylated anthocyanin, the separation and purification of acylated anthocyanin is difficult, and it is difficult to obtain target anthocyanin and other problems, to achieve the effect of good repeatability and large processing capacity

Active Publication Date: 2021-12-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this technical scheme discloses its separation and obtains two kinds of anthocyanins, but from figure 1 In the HPLC chart of the turbid grape juice, it can be seen that its anthocyanin composition is simple. From the limitation of high-speed countercurrent chromatography, it can be inferred that when the anthocyanin components of the separated sample are complex, it may be difficult to obtain the target anthocyanin by this method
[0008] Due to the difficulty in the separation and purification of acylated anthocyanins, there is currently no commercial standard acylated anthocyanins on the market

Method used

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  • A method for separating and preparing petunienin-3-o-(6-o-p-coumaroyl) glucoside
  • A method for separating and preparing petunienin-3-o-(6-o-p-coumaroyl) glucoside
  • A method for separating and preparing petunienin-3-o-(6-o-p-coumaroyl) glucoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Wash and peel the grapes to obtain 1 kg of grape skins, add 70% ethanol solution containing 0.5% (v / v) hydrochloric acid according to the ratio of solid to liquid 1g: 6mL, mix well, and extract by ultrasonic for 60min, (control the temperature below 50°C , protected from light), filtered through gauze, the filtrate was centrifuged at 4000rpm for 10min, and the supernatant was taken. The filter residue was extracted once more in the same way. The filtrates were combined and filtered one more time using a Buchner funnel. The filtrate was evaporated under reduced pressure at 45°C to remove ethanol and concentrated to obtain a crude grape skin anthocyanin extract. The high performance liquid chromatogram of grape skin anthocyanin crude extract is as follows figure 2 shown.

[0049] Put the AB-8 macroporous resin into the chromatographic column, and use ethanol, 0.5mol / L hydrochloric acid solution, 0.5mol / L sodium hydroxide solution, and water to wash in sequence, and th...

Embodiment 2

[0054] Wash and peel the grapes to obtain 4kg of grape skins, add 70% ethanol solution containing 0.5% (v / v) hydrochloric acid according to the ratio of solid to liquid 1g: 6mL, mix well, and ultrasonically extract for 90min, (control the temperature below 50°C , protected from light), filtered through gauze, the filtrate was centrifuged at 4000rpm for 10min, and the supernatant was taken. The filter residue was extracted once more in the same way. The filtrates were combined and filtered one more time using a Buchner funnel. The filtrate was evaporated under reduced pressure at 45°C to remove ethanol and concentrated to obtain a crude grape skin anthocyanin extract.

[0055] Put the AB-8 macroporous resin into the chromatographic column, and use ethanol, 0.5mol / L hydrochloric acid solution, 0.5mol / L sodium hydroxide solution, and water to wash in sequence, and then wash the crude anthocyanin extract at 0.2BV / The flow rate of h is injected into the chromatography column. A...

Embodiment 3

[0059] Wash and peel the grapes to obtain 10kg of grape skins, add 70% ethanol solution containing 0.5% (v / v) hydrochloric acid according to the ratio of solid to liquid 1g: 6mL, fully mix, ultrasonically extract for 60min, (control the temperature below 50°C , protected from light), filtered through gauze, the filtrate was centrifuged at 4000rpm for 10min, and the supernatant was taken. The filter residue was extracted once more in the same way. The filtrates were combined and filtered one more time using a Buchner funnel. The filtrate was evaporated under reduced pressure at 45°C to remove ethanol and concentrated to obtain a crude grape skin anthocyanin extract.

[0060] Put the AB-8 macroporous resin into the chromatographic column, and use ethanol, 0.5mol / L hydrochloric acid solution, 0.5mol / L sodium hydroxide solution, and water to wash in sequence, and then wash the crude anthocyanin extract at 0.2BV / The flow rate of h is injected into the chromatography column. Aft...

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Abstract

The invention discloses a method for separating and preparing petunia-3-O-(6-O-p-coumaroyl) glucoside through extraction, macroporous resin purification, extraction, high-speed countercurrent chromatography and solid-phase extraction purification and other steps to separate and purify from grapes to obtain high-purity petunienin-3-O-(6-O-p-coumaroyl) glucoside monomer. By this method, at least 80 mg of petunienin-3-O-(6-O-p-coumaroyl) glucoside can be obtained from 10 kg of grape skins, and the purity can reach no less than 98%. The method has the advantages of simple operation, large processing capacity and good repeatability, and provides a new idea for the development and utilization of grape resources in my country.

Description

technical field [0001] The invention relates to the technical field of separation and purification of natural products, in particular to a method for separating and preparing petunienin-3-O-(6-O-p-coumaroyl) glucoside. Background technique [0002] Grapes are the fruit of the plant Vitis vinifera and are cultivated all over the world. Grapes have high nutritional value. Chinese medicine believes that grapes are flat in nature and sweet in taste, can nourish qi and blood, strengthen the heart and diuresis. Modern research shows that grapes not only contain vitamin B1, vitamin B2, vitamin C, vitamin E and other vitamins, but also rich polyphenolic compounds such as resveratrol, proanthocyanidins, anthocyanins, etc. Tumor, antibacterial and other biological activities. [0003] Anthocyanins are one of the important polyphenols in grapes. Studies have shown that grapes contain anthocyanins formed by combining anthocyanins such as delphinidin, mallowin, paeoniflorin and petuni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/08C07H17/065
CPCC07H1/08C07H17/065
Inventor 陈卫谢佳宏徐阳崔昊昕
Owner ZHEJIANG UNIV
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