Exosome particle screening device based on ultracentrifugation technology

Abstract

The invention discloses an exosome particle screening device based on an ultracentrifugation technology. The exosome particle screening device comprises a working base, wherein a rotary table motor isfixedly arranged in the working base; a motor shaft is arranged on the rotary table motor in a power manner; a second rotary shaft is arranged in the working base; a fan-shaped rotary table is rotatably arranged in the second rotary shaft; a fan-shaped outer gear ring is fixedly arranged on the fan-shaped rotary table; a first rotary shaft is rotatably arranged in the working base; after a test tube filled with precipitates is mounted in equipment, the equipment automatically adds a quantitative PBS solution into the test tube, and automatically blows and beats the precipitates added with thePBS solution; the positions of centrifugal test tubes are slowly and alternately transferred while blowing and beating, so that the influence on the properties of the solution in the centrifugal testtubes due to too fast transfer is prevented; and a worker only needs to feed the test tube filled with the precipitates into the equipment and take out the finished centrifugal test tubes, so that automatic integration of adding of the PBS solution, blowing and beating of the solution and transfer of the solution is realized, working efficiency is improved, and working quality is guaranteed.

Description

technical field [0001] The invention relates to the technical field of exosome particle screening, in particular to an exosome particle screening device based on ultracentrifugation technology. Background technique [0002] The ultracentrifugation method is the most commonly used exosome purification method, which uses low-speed centrifugation and high-speed centrifugation alternately to separate vesicle particles of similar size. [0003] In the screening process of exosome particles using ultracentrifugation technology, one of the steps requires the initial removal of the supernatant to leave a precipitate rich in exosomes, after standing still, the residual liquid at the bottom is sucked out and discarded, and finally quantitative PBS is taken (Phosphate-buffered saline solution) was added to the sediment and repeatedly pipetted for one minute, and finally the solution was taken out and transferred to a 1.5ml centrifuge tube and left to stand for another minute. The whole...

AI Technical Summary

Problems solved by technology

[0003] In the screening process of exosome particles using ultracentrifugation technology, one of the steps requires the initial removal of the supernatant to leave a precipitate rich in exosomes, after standing still, the residual liquid at the bottom is sucked out and discarded, and finally quantitative PBS is taken (Phosphate buffered saline solution) was added to the sediment and blown repeatedly for one minute, and finally the solution was taken out and transferred to a 1.5ml centrifuge tube and left to stand for another minute. The whole process was manually operated, which consumed a lot of time and energy during the blowing and standing process, and It is difficult to accurately control the amount of PBS solution when using a dropper to draw PBS manually, so this device is designed to solve the above problems

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