Method for improving chlorella pyrenoidosa fermentation grease

A technology of chlorella pyrenoidosa and oil, which is applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems such as the application of chlorella pyrenoidosa which are rarely reported, and achieve the method is simple and easy to operate, reduce Effects of production cost and energy consumption reduction

Inactive Publication Date: 2021-01-26
QINGDAO KEHAI BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the above-mentioned prior art, Chlorella pyrenoidosa is used to adsorb heavy metals, but the applic...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for improving the high-yield oil and fat fermented by chlorella pyrenoidosa, comprising the following steps:

[0027] 1) Activation culture: inoculate Chlorella pyrenoidosa cells in the first medium for activation and culture to the logarithmic growth phase and serve as seed solution. The first medium is: glucose 10g / L, KNO 3 0.08g / L, K 2 HPO 4 ·3H 2 O 1.0g / L, MgSO 4 .7H 2 O0.3g / L, FeSO 4 .7H 2 O 0.003g / L, Vitamin B1 5-10g / L, A5 trace element solution 1mL / L.

[0028] Culture conditions: the test was carried out in a 250mL Erlenmeyer flask with a liquid volume of 100mL. The inoculum size was 10%, the culture temperature was 25°C, the initial pH was 6.5, the light intensity was 1500Lux, the shaker speed was 110r / min, and the fermentation culture period was 7d.

[0029] 2) Inoculate Chlorella pyrenoidosa into the second medium containing different glucose concentrations, using glucose as the carbon source, the concentration of amino acid as a fixed nitrog...

Embodiment 2

[0032] A method for improving the high-yield oil and fat fermented by chlorella pyrenoidosa, comprising the following steps:

[0033] 1) Activation culture: inoculate Chlorella pyrenoidosa cells in the first medium for activation and culture to the logarithmic growth phase and use it as a seed solution. The first medium is: glucose 10g / L, KNO 3 0.08g / L, K 2 HPO 4 ·3H 2O 1.0g / L, MgSO 4 .7H 2 O0.3g / L, FeSO 4 .7H 2 O 0.003g / L, Vitamin B1 5-10, A5 trace element solution 1mL / L.

[0034] Culture conditions: the test was carried out in a 250mL Erlenmeyer flask with a liquid volume of 100mL. The inoculum size was 10%, the culture temperature was 25°C, the initial pH was 6.5, the light intensity was 1500Lux, the shaker speed was 110r / min, and the fermentation culture period was 7d.

[0035] 2) Inoculate Chlorella pyrenoidosa into media containing different glucose concentrations, use sucrose as carbon source, and fix nitrogen source amino acid at a concentration of 0.08g / L. Th...

Embodiment 3

[0038] A method for improving the high-yield oil and fat fermented by chlorella pyrenoidosa, comprising the following steps:

[0039] 1) Activation culture: inoculate Chlorella pyrenoidosa cells in the first medium for activation and culture to the logarithmic growth phase and serve as seed solution. The first medium is: glucose 10g / L, KNO 3 0.08g / L, K 2 HPO 4 ·3H 2 O 1.0g / L, MgSO 4 .7H 2 O0.3g / L, FeSO 4 .7H 2 O 0.003g / L, Vitamin B1 5-10, A5 trace element solution 1mL / L.

[0040] Culture conditions: the test was carried out in a 250mL Erlenmeyer flask with a liquid volume of 100mL. The inoculum size was 10%, the culture temperature was 25°C, the initial pH was 6.5, the light intensity was 1500Lux, the shaker speed was 110r / min, and the fermentation culture period was 7d.

[0041] 2) Inoculate Chlorella pyrenoidosa into the second medium containing different glucose concentrations, using glucose as the carbon source, the concentration of amino acid as a fixed nitrogen ...

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Abstract

The invention discloses a method for improving chlorella pyrenoidosa fermentation grease, and belongs to the technical field of microalgae development. The method comprises the following steps: an activation culture step: inoculating culture medium with chlorella pyrenoidosa for activation culture to a logarithmic phase and taking the chlorella pyrenoidosa as a seed solution; inoculating a shake flask of a culture medium with the chlorella pyrenoidosa, and performing culturing in an illumination shaking table; and finally, centrifuging, washing and freeze-drying cultured microbial cells to obtain powder which is used for determining and evaluating the biomass oil concentration, the total fat content, the fat content and the yield of the chlorella pyrenoidosa. According to the method, the fatty acid composition of microbial oil is measured and analyzed by utilizing the influence of different culture medium compositions including a carbon source, a nitrogen source, a carbon-nitrogen ratio, divalent metal ions and different culture conditions such as temperature and initial culture pH value on chlorella cell growth and fat accumulation, and a gas chromatographic analysis result showsthat the fatty acid composition of the microbial oil can be accurately measured and analyzed. The content of unsaturated fatty acid reaches about 73%.

Description

technical field [0001] The invention relates to the technical field of microalgae development, in particular to a method for using chlorella pyrenoidosa to ferment oil. Background technique [0002] Microalgae is a type of single-celled algae, which has the advantages of fast cell proliferation, short production cycle, not limited by seasons and land, rich source of medium required, and the oil composition produced is similar to vegetable oil. Therefore, the use of The development of oil resources by microalgae has been increasingly favored by people. Microalgae are considered to be a potential biodiesel feedstock. [0003] Chlorella pyrenoidosa belongs to Chlorella phylum Chlorella, which is a universal single-cell green algae. Chlorella has a strong reproductive ability and can use sunlight to produce a large amount of protein. The protein content is about 50%, which exceeds that of beef, soybeans, etc. High-protein food; also contains 10% to 30% of unsaturated fatty aci...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12N1/12C12R1/89
CPCC12P7/649C12N1/12Y02E50/10
Inventor 李悦明徐建春徐炳政韩萍董婉
Owner QINGDAO KEHAI BIOLOGICAL
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