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Application of amentoflavone in preparation of drug for treating glioma

A technology of biflavonoids and glioma, applied in the field of medicine, can solve the problems that the prognosis of patients has not been significantly improved, and the treatment of glioblastoma has not achieved the desired effect.

Pending Publication Date: 2021-01-26
NINGXIA MEDICAL UNIV
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Problems solved by technology

[0003] So far, although surgery, radiotherapy and chemotherapy have made great progress, the treatment of glioblastoma is far from ideal. The prognosis of patients has not improved significantly, and the average survival time is only about 1 year

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  • Application of amentoflavone in preparation of drug for treating glioma
  • Application of amentoflavone in preparation of drug for treating glioma
  • Application of amentoflavone in preparation of drug for treating glioma

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Embodiment Construction

[0012] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0013] 1 Materials and methods

[0014] 1.1 Cell culture The GBM cell line U87MG was purchased from the Shanghai Cell Resource Center of the Chinese Academy of Sciences. It is a monoclonal line of human glioblastoma multiforme, and the WHO grading standard is grade IV. Cultured in DMEM medium containing 10% fetal bovine serum (DMEM medium, trypsin were purchased from Gibco, USA, and fetal bovine serum was purchased from HyClone, USA).

[0015] 1.2 The main reagents (boxes) were purchased from Sigma Company of the United States, JAK2, P-JAK2, STAT3, P-STAT3, Bcl-2, and β-actin monoclonal antibodies were purchased from Cell Signal Technology Company of the United States, and Western blotting related reagents ( Kit) was purchased from Nanjing Kaiji Biotechnology Development Co., Ltd., the luminescent liquid was...

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Abstract

The invention discloses application of amentoflavone in preparation of a drug for treating glioma. The amentoflavone is used for influencing activation of a JAK2STAT3 pathway of glioblastoma and apoptosis and proliferation of tumor cells. The method comprises the following steps: treating glioblastoma cell lines U87MG 24, 48 and 72 hours by using 0 [mu]M, 50 [mu]M, 100 [mu]M, 150 [mu]M and 200 [mu]M amentoflavone, then respectively detecting the influence of the glioblastoma cell lines on cell proliferation and apoptosis by using CCK8 and Annexin V / PI staining, and detecting the influence of the glioblstoma cell lines on cell JAK2-STAT3 pathway activation and the apoptosis related protein of the downstream regulation by using Western blotting. The amentoflavone is used for blocking a JAK2-STAT3 signal path, promoting tumor cell apoptosis and inhibiting proliferation of tumor cells, and is a novel method for glioblastoma treatment or adjuvant therapy.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a new application of an extract, and the application of amiflavone of spicantha spiconiae in the preparation of drugs for treating glioma. Background technique [0002] Glioma is the most common primary tumor of the central nervous system, and glioblastoma is the most malignant among them, showing infiltrative growth and rapid growth, which is extremely difficult to cure. Glioblastoma, also known as glioblastoma multiforme (GBM), is the most common and deadly primary malignant tumor in the adult central nervous system, and the current treatment effect is poor. Studies have shown that the abnormally activated Janus kinase 2 (Jak2)-signal transducers and activators of transcription 3 (STAT3) pathway plays an important role in the pathogenesis of GBM, and it is a clinical One of the important potential targets for comprehensive therapy. Amentoflavone, also known as amentoflavone (A...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P35/00
CPCA61K31/352A61P35/00
Inventor 顾金海牛建国文玉军孙涛王峰和祯泉强媛媛王鹏杨勇
Owner NINGXIA MEDICAL UNIV
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