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Application of MST1 as drug target in preparation of drug for treating colorectal cancer

A technology of colorectal cancer and drugs, applied in the field of biomedicine, can solve the problem of few clinical significance studies

Pending Publication Date: 2021-01-15
THE FIRST AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the expression of MST1 in CRC and its clinical significance have been rarely studied

Method used

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  • Application of MST1 as drug target in preparation of drug for treating colorectal cancer
  • Application of MST1 as drug target in preparation of drug for treating colorectal cancer
  • Application of MST1 as drug target in preparation of drug for treating colorectal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Cell Culture and Transfection

[0023] 1 Materials and methods

[0024] 1.1 General information

[0025] A total of 81 colorectal cancer patients (tumor tissue and paired normal adjacent tissue) were collected from the Department of General Surgery of Nanchong Central Hospital from March 2009 to April 2014. This study has been approved by the Medical Ethics Committee and complies with the relevant requirements of the Declaration of Helsinki of the World Medical Association. All patients were aware of the research content and signed the informed consent.

[0026] 1.2 Cell culture and transfection

[0027] HCT116, SW480, LOVO and human colonic epithelial cells HCoEpiC were obtained from the Chinese Academy of Sciences (Shanghai) cell bank, and HCT116, SW480, LOVO cells were cultured in RPMI-1640 medium (Gibco, USA) with 10% fetal bovine serum. LV-MST1 and LV-NC lentiviral vectors were transfected according to the manufacturer's instructions (Gene Pharmaceuti...

Embodiment 2

[0049] The expression of embodiment 2MST1 in CRC tissue

[0050] MST1 is lowly expressed in CRC tissues. First, 275 cases of CRC tissues and 349 cases of normal tissues were analyzed (http: / / gepia.cancer-pku.cn / index.html) to evaluate the level of MST1 in CRC. The results are shown in figure 1 .

[0051] figure 1 Middle A is the mRNA expression of MST1 in the TCGA database (colorectal cancer and adjacent non-cancerous tissues); B is the mRNA expression of MST1 in CRC and adjacent non-cancerous tissues; C is the protein expression of MST1 in adjacent cancerous tissues and adjacent non-cancerous tissues; D is the expression of MST1 in cells; *P<0.05, **P<0.01.

[0052] according to figure 1 The detection results showed that, compared with normal tissues, the expression of MST1 in CRC tissues was lower ( figure 1 A), qRT-PCR and Western blot method were used to detect MST1 expressing cells in CRC tissues. The mRNA level of MST1 in CRC tissues was significantly lower than tha...

Embodiment 3

[0053] Example 3 Correlation between immunohistochemical detection of MST1 and clinicopathological features of CRC

[0054] Immunohistochemical results such as figure 2 as shown, figure 2 Among them, A is the immunohistochemical analysis of MST1 expression in CRC and adjacent tissues; B is the statistical analysis of MST1 expression in CRC and adjacent tissues.

[0055] according to figure 2 The detection results showed that the low expression rate of MST1 in CRC was 61.1% (51 / 81) (Figure 2A and B). In addition, the positive expression of MST1 was mainly concentrated in the cytoplasm. By analyzing the relationship between MST1 and clinicopathological features of CRC patients, we found that low MST1 expression was positively correlated with tumor size (P=0.011) and differentiation (P<0.001).

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Abstract

The invention discloses an application of MST1 as a drug target in preparation of a drug for treating colorectal cancer. According to the application of the MST1 as the drug target in preparation of the drug for treating the colorectal cancer, the drug is a drug for inhibiting colorectal cancer migration, invasion and proliferation by promoting expression of the MST1. According to the invention, the proliferation, invasion and migration of CRC cells are inhibited by improving the expression of the MST1, and the MST1 can regulate EMT through a Hippo signal path and inhibit the proliferation, invasion and migration of the CRC cells, so that the effect of cancer suppressor genes is exerted.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of MST1 as a drug target in the preparation of drugs for treating colorectal cancer. Background technique [0002] Colorectal cancer (CRC) is a common malignant tumor of the gastrointestinal tract, and its early symptoms are not obvious. With the development of the disease, there will be changes in bowel habits, blood in the stool, diarrhea, alternating diarrhea and constipation, local abdominal pain and other symptoms. Colorectal cancer is the third most common cancer in China after lung cancer and gastric cancer. However, the process of CRC from precancerous lesion (adenoma) to malignant lesion is a long process, and it is one of the few malignant tumors that can be detected and treated early. [0003] As the core kinase of Hippo signaling pathway, macrophage stimulating 1 (MST1) regulates cell proliferation and apoptosis by interacting with dow...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61P35/00A61P35/04C12Q1/6886
CPCA61K45/00A61P35/00A61P35/04C12Q1/6886C12Q2600/158C12Q2600/106C12Q2600/118
Inventor 潘雪
Owner THE FIRST AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIVERSITY
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