SNP loci for increasing content of fatty acid C14: 0 in chicken and method for breeding high-quality chicken strain

A fatty acid and chicken technology, which is applied in the field of molecular biology, can solve the problems of lack of universality of multi-variety effects, impossibility of livestock breeding practice, and has not yet been carried out, and achieve the effects of high accuracy, low cost, and simple operation

Active Publication Date: 2021-01-08
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, the effects of these SNPs are mostly limited to a specific domestic animal breed, and none of them have the universality of multi-breed effects within a species, which makes them unable to be widely used in livestock breeding practices
For chickens, research in related fields lags behind livestock, and large-scale GWAS analysis related to fatty acid metabolism has not yet been carried out

Method used

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  • SNP loci for increasing content of fatty acid C14: 0 in chicken and method for breeding high-quality chicken strain
  • SNP loci for increasing content of fatty acid C14: 0 in chicken and method for breeding high-quality chicken strain
  • SNP loci for increasing content of fatty acid C14: 0 in chicken and method for breeding high-quality chicken strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. Determination of SNP markers related to fatty acid C14:0 content in chicken

[0039] 1. Genome-wide association analysis (GWAS) obtained SNP molecular markers in the upstream region of FASN gene and chicken fatty acid C14:0 content.

[0040] 1) Experimental animals

[0041]The present invention uses populations from the sixteenth generation chicken IMF selection line (n=256) and control line (n=264), totaling 520 hens. The IMF selection line and the control line are derived from the same basic group (Jingxing Yellow Chicken, Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences). Since 2000, IMF traits have been selected for directional selection. Compared with the control line, the IMF content of the IMF-selected line population was significantly increased (P<0.001), and the shear stress was significantly reduced after selection (ZHAO et al., 2007). During the feeding process, free access to food and water ...

Embodiment 2

[0060] Example 2 The effectiveness and contribution rate of chr18:4910701 and chr18:4911141 mutation-associated fatty acid C14:0 content in chicken meat

[0061] 1. Chr18:4910701 and chr18:4911141 regulate the expression of the associated gene FASN

[0062] 1) Experimental animals

[0063] Using the same animal population as in the GWAS analysis in Example 1 (the sixteenth generation chicken IMF selection line, n=252; and the control line, n=264), the relevant data were used for linkage and variation contribution analysis; Ten mutant individuals and reference individuals carrying the chr18:4910701 site and the chr18:4911141 site were used to detect the expression of the FASN gene.

[0064] 2) The linkage relationship between chr18:4910701 and chr18:4911141 loci

[0065] Use the haplotype linkage analysis method to explore the relationship between the chr18:4910701 site and the chr18:4911141 site, and find that there is a high linkage between the two, such as Figure 4 shown...

Embodiment 3

[0085] Example 3 Establishment of molecular marker detection method for loci chr18:4910701 and chr18:4911141 and their application in breeding

[0086] 1. Establishment of molecular marker detection method

[0087] (1) Primer design

[0088] According to the chicken chromosome 18 DNA sequence provided by the Ensemble website (version 6.0), 2 pairs of specific primers containing these 2 SNP sites were designed using NCBI primer design software. The DNA sequences of the primers are as follows:

[0089] chr18:4910701

[0090] Upstream primer: 5'-TCTTCCCAGGAAGGCGAATTT-3' (SEQ ID NO.1)

[0091] Downstream primer: 5'-AGAATCACGGGATGGTTGGAG-3' (SEQ ID NO.2)

[0092] chr18:4911141

[0093] Upstream primer: 5'-TGGAGGAGGTGAATTCTCACAG-3' (SEQ ID NO.3)

[0094] Downstream primer: 5'-TTTCGGTCGGCAAAAGCG-3' (SEQ ID NO.4)

[0095] (2) PCR reaction program optimization

[0096] The reaction program is: 95°C for 10min, 95°C for 30s, 60°C for 30s, 72°C for 50s, a total of 32 cycles; 72°C ...

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Abstract

The invention provides SNP loci related to the content of fatty acid C14: 0 (myristic acid) in chicken on a chicken chromosome 18 and application of the SNP loci, provides a kit for detecting SNP molecular markers, and further provides a method for screening high-quality chicken strains by utilizing the two linked SNP loci. The chicken quality is improved by increasing the C14: 0 content of the chicken, and the genetic improvement progress of the broiler chicken is accelerated. The kit is simple to operate, high in sensitivity, high in accuracy and low in detection cost, and has important application value.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a SNP marker related to chicken fatty acid C14:0 content, a kit for detecting the marker, and an application of the SNP marker and the kit to high-quality chicken breeding. Background technique [0002] As a big chicken production and consumption country, my country ranks second in the world in terms of chicken production and consumption. Chicken has become my country's second largest meat consumption product and an important source of animal protein. However, with the continuous improvement of the growth rate and feed conversion efficiency of broilers, the quality problems of chicken products have become increasingly prominent, and it is difficult to meet the growing demand of consumers for high-quality livestock products. Increasing the content of intramuscular fat is an important means to improve the quality of livestock and poultry meat, and fatty acids are the main...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156Y02P60/87
Inventor 文杰赵桂苹崔焕先郑麦青刘冉冉李庆贺王巧
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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