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Plasma exosome tsRNA marker related to non-obstructive azoospermia diagnosis and application thereof

A technology of azoospermia and markers, which is applied in the direction of DNA/RNA fragments, recombinant DNA technology, and microbial measurement/testing, and can solve the problems of ineffective diagnosis of non-obstructive azoospermia

Active Publication Date: 2021-01-05
XUZHOU MEDICAL UNIV
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Problems solved by technology

[0005] One of the purposes of the present invention is to overcome the deficiency that the existing non-invasive diagnostic indicators cannot effectively diagnose non-obstructive azoospermia, and provide a plasma exosomal tsRNA marker related to the diagnosis of non-obstructive azoospermia

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  • Plasma exosome tsRNA marker related to non-obstructive azoospermia diagnosis and application thereof
  • Plasma exosome tsRNA marker related to non-obstructive azoospermia diagnosis and application thereof
  • Plasma exosome tsRNA marker related to non-obstructive azoospermia diagnosis and application thereof

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Embodiment Construction

[0026] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0027] Such as figure 1 As shown: the present invention first analyzes the differential tsRNA in plasma exosomes of non-obstructive azoospermia, obstructive azoospermia, and healthy control population, and then analyzes the selected differential tsRNA in more non-obstructive azoospermia, obstructive azoospermia, Real-time fluorescent quantitative PCR verification was carried out in samples from patients with sexual azoospermia, severe spermatogenic dysfunction and healthy controls, and finally determined whether the screened tsRNA was present in testicular tissue samples from patients with obstructive azoospermia and non-obstructive azoospermia. There are differences in expression to further determine its feasibility as a diagnosis of non-obstructive azoospermia, and to distinguish non-obstructive azoospermia from obstructive azoosper...

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Abstract

The invention discloses a plasma exosome tsRNA marker related to non-obstructive azoospermia diagnosis and application thereof. The marker is tRF-Gly-GCC-002, wherein the sequence of the tRF-Gly-GCC-002 is shown as SEQ ID NO.1. The marker has specificity and sensitivity to non-obstructive azoospermia, and can be used for preparing a diagnostic kit for non-obstructive azoospermia; and the plasma exosome marker tRF-Gly-GCC-002 is differentially expressed in plasma of patients with non-obstructive azoospermia and obstructive azoospermia, the plasma exosome marker tRF-Gly-GCC-002 can well diagnosethe non-obstructive azoospermia, and can predict the azoospermia sperm taking result, and the effect of the plasma exosome marker tRF-Gly-GCC-002 is superior to common indexes such as the plasma follicle stimulating hormone level and the testicular volume.

Description

technical field [0001] The invention belongs to the field of biological diagnosis and medicine, and specifically relates to a plasma exosome tsRNA marker related to the diagnosis of non-obstructive azoospermia and its application. Background technique [0002] The incidence of azoospermia accounts for 10%-20% of male infertility. According to the presence or absence of sperm in the testis, it can be divided into two categories: one is obstructive azoospermia (OA) with normal spermatogenesis in the testis. The other is non-obstructive azoospermia (NOA), which is a disorder of testicular spermatogenesis. An accurate diagnosis of non-obstructive azoospermia can provide valuable guidance to clinicians. Although conventional biochemical markers in semen, such as citric acid, acid phosphatase, fructose, and α-glucosidase, can be used to assist in the diagnosis of OA patients; testicular volume, serum follicle-stimulating hormone (FSH) levels, etc. can be used to assist in the di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/113C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 朱作斌李颖韩从辉
Owner XUZHOU MEDICAL UNIV
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