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Bacillus velezensis Hsg1949 and application of bacillus velezensis Hsg1949

The technology of bacillus and pathogen is applied in the field of biological control, can solve the problems such as the application of peanut fruit rot which has not been found, and achieves the effects of easy popularization and application, low cost and simple preparation method

Active Publication Date: 2021-01-05
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacillus Velez ( Bacillus velezensis) Belonging to a new species of Bacillus, it has been used to control rice blast, cotton verticillium wilt, rice stripe, sugarcane head blight, lettuce soft rot, pear gray mold, tobacco red spot fungus, banana wilt, Apple rot and other plant diseases, but its application in the prevention and treatment of peanut fruit rot has not been found

Method used

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  • Bacillus velezensis Hsg1949 and application of bacillus velezensis Hsg1949
  • Bacillus velezensis Hsg1949 and application of bacillus velezensis Hsg1949
  • Bacillus velezensis Hsg1949 and application of bacillus velezensis Hsg1949

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Experimental program
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Effect test

Embodiment 1

[0014]Example 1 Isolation and screening of bacteria with antagonistic effect on peanut fruit rot

[0015]In October 2019, the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences collected more than 1,500 soil samples from plots with different incidences of peanut fruit rot in Daming, Shijiazhuang, Baoding, Qinhuangdao, Tangshan and other counties and cities in Hebei, and brought them back to the experiment. After the chamber, the bacillus was separated and separated and purified several times on the LB medium to obtain pure cultured bacteria. Using peanut fruit rot as the indicator bacteria, the plate confrontation method was used to screen the strains with high antagonistic performance.

[0016]Specific steps: Weigh 1g of the above-mentioned peanut field soil samples and place them into sterile Erlenmeyer flasks containing 100mL sterile water, shake at 170rpm for 30min, and let stand for 30min. Take 10mL of supernatant and add it to a 50mL sterile centrifuge...

Embodiment 2

[0017]Example 2 Determination of genetic stability of antagonistic strains

[0018]The strain Hsg1949 with the strongest antagonistic effect was subcultured on LB medium by streaking method. After 40 generations of culture, the plate confrontation method was used to determine the antagonistic effect of the strain against Fusarium peanut fruit rot. The original strain was taken as In contrast, the antagonistic strain has the same antagonistic effect as the original strain after 40 generations of culture (Table 1), indicating that the antagonistic strain has good genetic stability. The antagonistic strain with stable antibacterial effect is scraped and placed in 1mL 25% Store the sterile glycerin solution in a cryotube at -80°C.

Embodiment 3

[0019]Example 3 Identification of strain Hsg1949

[0020](1) Identification of morphological characteristics

[0021]According to the experimental content and experimental methods recorded in the "Manual for Identification of Common Bacterial Systems", the antagonistic strain Hsg1949 was identified. The colony morphology was observed on LB medium. After the strain was streaked, the strain was inoculated at a constant temperature of 28°C. Incubate for 24 hours to observe the colony morphology. The colony of strain Hsg1949 is nearly round, with eroded edges, umbilical protrusions, round, neat edges, and the colony is raised like a steamed bread, and the surface is moist; in the later period of culture, the surface is dry and wrinkled, and the color is milky white, dull and opaque. Not tightly bound to the medium. Pick and cultivate the bacterial moss for 48 hours and then observe it under a 100-fold oil microscope under an optical microscope. It can be seen under the microscope that the str...

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Abstract

The invention discloses bacillus velezensis Hsg1949, and the preservation number of the bacillus velezensis Hsg1949 is CGMCC No. 20836. The antibacterial rate of the bacillus velezensis Hsg1949 on thepeanut fruit rot reaches 58.7-72.9%, and the field prevention and treatment effect on the peanut fruit rot can reach 71.24%. The bacillus velezensis disclosed by the invention can be prepared into acorresponding microbial agent to be applied to a peanut field, so that the occurrence of peanut fruit rot can be effectively prevented. Besides, the bacillus velezensis Hsg1949 disclosed by the invention is wide in antibacterial spectrum, and has a certain inhibition effect on peanut fruit rot pathogens, peanut southern blight pathogens, cotton verticillium wilt pathogens, cotton fusarium wilt pathogens, cotton rhizoctonia solani and the like.

Description

Technical field[0001]The invention relates to the field of biological control, in particular to a Velez Bacillus Hsg1949, and also relates to the use of the Velez Bacillus to control peanut fruit rot and other plant diseases.Background technique[0002]Fruit rot is an important disease in peanut production. The main symptom of peanut fruit rot is peanut fruit rot. The light part of the pod is brown or black, the kernel is small and hard, and the serious pod is dark black with the skin. And the nuts are rotted, usually whole plant or spot pieces. Peanut-infected plots have reduced yields by 20%, and severely reduced yields by more than 50%, posing an extremely serious threat to the yield and quality of peanuts. Fusarium is one of the main pathogens that cause peanut fruit rot. At present, it is mainly controlled by chemical fungicide pesticides, but the environmental pollution is serious and the control effect is not good. Finding an economic, safe, and effective control measure is a t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCA01N63/22C12N1/205C12R2001/07
Inventor 曹伟平宋健鹿秀云赵聚莹陈丹
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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