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Bifidobacterium animalis and breeding method and application thereof

A technology of animal bifidobacteria and bifidobacteria, applied in the direction of bifidobacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve problems affecting the intestinal micro-ecological balance, and improve the intestinal micro-environment, Improve intestinal health and enhance immunity

Active Publication Date: 2021-01-05
SHANDONG DEYI DAIRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Intestinal abnormalities can be treated with medication, but it can affect the microecological balance of the gut, and taking medication is not a healthy and sustainable way for most people

Method used

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  • Bifidobacterium animalis and breeding method and application thereof
  • Bifidobacterium animalis and breeding method and application thereof
  • Bifidobacterium animalis and breeding method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Sample Collection:

[0023] In April 2007, I went to Bama County, Guangxi Zhuang Autonomous Region to investigate the living conditions of the long-lived elderly and collected fecal samples from the elderly.

[0024] As shown in Table 1, all the people in group M come from Jiazhuan Township, where the long-lived elderly in the suburbs of Bama are concentrated. Group S is the direct or collateral generation of group M, who also live in Jiazhuan Township. Group C comes from the Bama County Welfare Institute. And Baxu City Village.

[0025] Among them, group M is the long-lived elderly living in Jiazhuan Township in the suburb of Bama, group S is the same generation of long-lived elderly living in the suburb of Bama, and group C is the elderly living in Bama County.

[0026] Table 1 Grouping of the sampled population

[0027]

[0028]

[0029] The 8 centenarians in Group M came from Ping'an Village, Poyue Village and other villages in Jiazhuan Township, among which...

Embodiment 2

[0034] Molecular Biological Species Identification of Bifidobacterium animalis:

[0035] 1. Use the bacterial genome extraction kit to extract the genomic DNA of potential bifidobacterium strains.

[0036] 2. Use 27F and 1541R primers to PCR amplify the complete sequence of bacterial 16S rDNA, and the product is recovered and purified by a DNA purification kit and then sent to Invitrogen (Beijing) Company for sequencing.

[0037] 3. Input the sequencing results into the NCBI database, and identify the strain species through BLASTn homology comparison.

[0038] The sequencing result is:

[0039]

[0040]

[0041] 4. Comparison:

[0042] The above 16s rDNA sequence was compared on NCBI, and it was found that the homology between the sequence and the 16s rDNA sequence of Bifidobacterium animalis subsp. lactis strain 2601 was 99%, and the E value was 0. Therefore, it can be determined that the isolated strain is Bifidobacterium animalis.

[0043] 5. Preservation:

[004...

Embodiment 3

[0046] Strain safety evaluation:

[0047] Safety evaluation is a prerequisite for the application of probiotics, and Bifidobacterium animalis IU-100 is a widely studied potential probiotic. The invention evaluates the safety of the strain in vitro and in vivo, and provides basic guarantee for the application of the strain.

[0048] The antibiotic resistance sensitivity and biogenic amine production of IU-100 were evaluated in vitro; the oral toxicity and translocation risk of IU-100 were evaluated in vivo.

[0049] The results showed that the strain IU-100 did not have strain-specific antibiotic sensitivity, which excluded the risk of lateral transfer of antibiotic resistance; no strain was found to produce biogenic amines after being cultivated in decarboxylase medium; the intragastric test found that 10 8 、10 9 、10 10 After feeding cfu / mouse for 28 days, no adverse reactions occurred in the mice, and the strain was not found in the blood, kidney, and liver. The study fou...

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Abstract

The invention relates to bifidobacterium animalis and a breeding method and application thereof, and belongs to the technical field of bifidobacterium lactis. According to the bifidobacterium animalisdisclosed by the invention, the strain is bifidobacterium animalis IU100 in the bifidobacterium genus, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. 12942. The bifidobacterium animalis strain is a bifidobacterium strain obtained by continuously researching intestinal floras of the longevous in Bama district in Guangxi of China formany years, selecting elder excrement samples with a high intestinal bifidobacteria quantity and carrying out separation and purification by utilizing a selective culture method. The bifidobacteriumanimalis disclosed by the invention not only can improve intestinal health conditions of populations, but also has a potential effect of improving immunity; and meanwhile, the invention provides a simple and feasible breeding method and application. The breeding method is high in efficiency and low in cost.

Description

technical field [0001] The invention relates to animal bifidobacteria, a breeding method and application thereof, and belongs to the technical field of lactobacillus bifidobacteria. Background technique [0002] Probiotics are a type of active microorganisms that can improve the host's micro-ecological balance and exert beneficial effects on the human body. They are widely found in the intestines of humans and animals, breast milk, and traditional fermented foods. Probiotics colonize the human body, such as the intestinal tract, and play an important physiological role in the digestion and absorption of nutrients, the growth and development of the body, immunity and antagonism. In a normal adult, the intestinal tract contains more than 1,000 species of microorganisms, which can be divided into three categories: native flora, conditional pathogens, and transit bacteria. The original flora is the intestinal dominant bacteria with immune regulation and other functions; the con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A23C9/123C12R1/01
CPCC12N1/20C12N1/02A23C9/1234C12R2001/01C12N1/205A23V2400/515
Inventor 徐轲栾庆刚彭跃屈倩王培亮
Owner SHANDONG DEYI DAIRY IND
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