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Uniform mixing device for DNA extraction

A technology of mixing and height adjustment devices, which is applied in the direction of DNA preparation, recombinant DNA technology, mixers with rotating stirring devices, etc., can solve the problems of increasing the work intensity of testing personnel and difficulty in ensuring the mixing effect, and achieves the goal of mixing Good effect, reduce working intensity and improve mixing effect

Active Publication Date: 2021-01-01
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, it is generally dependent on the inspectors for manual mixing, which greatly increases the work intensity of the inspectors, and it is difficult to guarantee the mixing effect.

Method used

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  • Uniform mixing device for DNA extraction
  • Uniform mixing device for DNA extraction
  • Uniform mixing device for DNA extraction

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Embodiment Construction

[0029] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0030] see Figure 1-7, in an embodiment of the present invention, a mixing device for DNA extraction, comprising a mixing box 1, a first valve 2, a first feeding funnel 3, a sealing cover 4, a temperature regulating box 5, a first motor 6, a first Pulley 7, second pulley 8, second valve 9, second feed hopper 10, stirring shaft 11, first splint 12, switch 13, first mixing device 14, third valve 15, fourth valve 16, the first A vertical plate 17, a fifth valve...

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Abstract

The invention discloses a uniform mixing device for DNA extraction. The uniform mixing device comprises a first motor, a water pump, uniform mixing blades, a temperature adjusting box and a uniform mixing box; the first motor rotates to drive a first belt wheel to rotate, so that a second belt wheel is driven to rotate, stirring blades on a stirring rotating shaft are driven to rotate, and a solution in the uniform mixing box is uniformly mixed; the uniform mixing blades rotate together in the rotating process of the stirring blades, so that the effect of uniformly mixing a solution in the uniform mixing box is improved; the temperature of the solution in the temperature adjusting box is adjusted by utilizing a heat conduction medium through a heat conduction tube, the solution in the temperature adjusting box further enters the uniform mixing box through the liquid guide tube, and manual uniform mixing of detection personnel is not needed, so that the working intensity of a detectionpersonnel is highly lowered; after uniform mixing is completed, containers such as test tubes and the like are placed between a first clamping plate and a second clamping plate, the second clamping plate moves towards the first clamping plate under the action of the elastic force of a spring, and therefore the containers such as the test tubes and the like are clamped and limited, and DNA extraction by the detection personnel is facilitated.

Description

technical field [0001] The invention relates to a mixing device, in particular to a mixing device for DNA extraction. Background technique [0002] DNA extraction is mainly the CTAB method, and other methods include physical methods such as glass bead method, ultrasonic method, grinding method, and freeze-thaw method. Chemical methods such as guanidine isothiocyanate method, alkaline lysis method. Biological method: enzymatic method. According to different nucleic acid separation and purification methods, there are siliceous materials, anion exchange resins, etc. The extraction methods of genomic DNA of different organisms (plants, animals, microorganisms) are different, but mainly all are adding solutions such as amyl alcohol, amyl alcohol and chloroform, and further mixing. [0003] At present, it is generally relying on the inspectors to perform manual mixing, which greatly increases the work intensity of the inspectors, and at the same time, it is difficult to guarant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10B01F13/00B01F15/00B01F15/06B01F7/20B01F7/16B01F27/906
CPCC12N15/1003B01F27/85B01F27/906B01F33/501B01F35/2215B01F35/4111B01F2035/99B01F35/92
Inventor 林风武赵岩孙宏斌张文龙王振兴程燊薛锦儒许哲男刘政甲李琳邹青旭郭博王晨
Owner JILIN UNIV
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