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Method for producing porous three-dimensional material by enzymatic degradation of PCL/PLLA polymer

A polymer and three-dimensional technology, applied in the biochemical treatment of enzymes/microorganisms, conjugated synthetic polymer artificial filaments, biochemical fiber treatment, etc., can solve the problems of less pore structure and no potential for ideal scaffolds, etc., and meet low requirements Effect

Pending Publication Date: 2020-12-29
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its poor pore structure does not have the potential to prepare ideal scaffolds

Method used

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  • Method for producing porous three-dimensional material by enzymatic degradation of PCL/PLLA polymer
  • Method for producing porous three-dimensional material by enzymatic degradation of PCL/PLLA polymer
  • Method for producing porous three-dimensional material by enzymatic degradation of PCL/PLLA polymer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for producing porous three-dimensional materials by enzymatically degrading PCL / PLLA polymers, comprising the following steps:

[0025] Step 1, dissolve PCL and PLLA with a mass ratio of 7:3 in a mixed solvent of dichloromethane and N,N-dimethylamide (the volume ratio of dichloromethane and N,N-dimethylamide is 3:1 ), obtain the PCL / PLLA solution of 10% by mass percentage;

[0026] Step 2, put the PCL / PLLA solution into a high-voltage electrospinning device for electrospinning. The technical parameters of the electrospinning are: spinning voltage 20kv, needle distance 20cm, drum speed 3000r / min, and the pump moves The speed is 50μm / s to obtain PCL / PLLA fiber membrane;

[0027] Step 3: Rinse the PCL / PLLA fiber membrane with alcohol and dry it thoroughly in a vacuum oven at a preset temperature of 40°C, and then place it in PBS buffer solution with esterase for degradation. The degradation time is 8 days. The concentration of the enzyme is 0.1mg / ml, the degrada...

Embodiment 2

[0029] A method for producing porous three-dimensional materials by enzymatically degrading PCL / PLLA polymers, comprising the following steps:

[0030] Step 1, dissolve PCL and PLLA with a mass ratio of 10:1 in a mixed solvent of dichloromethane and N,N-dimethylamide (the volume ratio of dichloromethane and N,N-dimethylamide is 4:1 ), obtain the PCL / PLLA solution of 5% by mass percent;

[0031] Step 2, put the PCL / PLLA solution into a high-voltage electrospinning device for electrospinning. The technical parameters of the electrospinning are: spinning voltage 10kv, needle distance 30cm, drum speed 2000r / min, and the pump moves The speed is 0.1μm / s, and the PCL / PLLA fiber membrane is obtained;

[0032] Step 3: Rinse the PCL / PLLA fiber membrane with alcohol and dry it thoroughly in a vacuum oven at a preset temperature of 40°C, and then place it in PBS buffer solution with esterase added for degradation. The concentration of esterase is 0.5mg / ml, the degradation temperature i...

Embodiment 3

[0034] A method for producing porous three-dimensional materials by enzymatically degrading PCL / PLLA polymers, comprising the following steps:

[0035]Step 1, dissolve PCL and PLLA with a mass ratio of 1:1 in a mixed solvent of dichloromethane and N,N-dimethylamide (the volume ratio of dichloromethane and N,N-dimethylamide is 5:1 ), obtain the PCL / PLLA solution of 15% by mass percent;

[0036] Step 2, put the PCL / PLLA solution into a high-voltage electrospinning device for electrospinning. The technical parameters of the electrospinning are: spinning voltage 15kv, needle distance 25cm, drum speed 1000r / min, and the pump moves The speed is 100 μm / s to obtain PCL / PLLA fiber membrane;

[0037] Step 3, rinse the PCL / PLLA fiber membrane with alcohol and place it in a vacuum oven at a preset temperature of 40°C to dry thoroughly, and then place it in PBS buffer solution with esterase added for degradation. The concentration of esterase is 0.01mg / ml, the degradation temperature wa...

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Abstract

The invention belongs to the technical field of bioengineering carrier materials, and particularly discloses a method for producing a porous three-dimensional material through enzymatic degradation ofa PCL / PLLA polymer. The method comprises the following steps of dissolving PCL and PLLA in a mixed solvent of dichloromethane and N,N-dimethylformamide to obtain a PCL / PLLA solution; carrying out electrostatic spinning on the solution to obtain a PCL / PLLA fibrous membrane; and placing the fibrous membrane in a PBS buffer solution added with esterase to be degraded, so that the porous three-dimensional PCL / PLLA fibrous membrane with the mechanical property not obviously reduced is obtained. The porous three-dimensional PCL / PLLA fibrous membrane is expected to serve as a high-quality raw material for preparing a porous tissue engineering scaffold or water / air treatment. According to the method, by adjusting the concentration and degradation time of the esterase, the purpose of controlling the size and number of micropores in the surface of the PCL / PLLA fibrous membrane can be achieved, and a new thought is provided for preparing porous materials with high porosity.

Description

technical field [0001] The invention relates to the technical field of bioengineering carrier materials, in particular to a method for producing porous three-dimensional materials by enzymatically degrading PCL / PLLA polymers. Background technique [0002] According to the "China Cardiovascular Disease Report", the current number of cardiovascular disease patients in China is about 290 million. In the treatment of cardiovascular diseases, it has become an important research field of bioengineering to manufacture cardiac stents and vascular stents to replace damaged parts based on biological and engineering techniques. An ideal scaffold should usually have the following characteristics: (1) three-dimensional high-porosity structure, which can facilitate the transport of nutrients and metabolites and cell growth; (2) biocompatible, degradable, and the degradation rate can be adjusted and matched The regeneration speed of cells and tissues in the body; (3) certain mechanical pr...

Claims

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Application Information

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IPC IPC(8): D04H1/4382D04H1/4374D04H1/728D01D5/00D01F8/14D06M16/00D06M101/32
CPCD04H1/4382D04H1/4374D04H1/728D01D5/003D01D5/0092D01D5/0069D01F8/14D06M16/003D06M2101/32
Inventor 刘艳萍曾鋆武跃文王颖超齐致远张梦楠田楠李倩
Owner ZHENGZHOU UNIV
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