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Experimental system for detecting influence of rhizosphere microorganisms on plant growth and root development and application thereof

A technology for plant growth and promotion of plant growth, which is applied in the field of experimental systems for studying the interaction between plants and rhizosphere microorganisms, and in the field of experimental systems for detecting the effects of rhizosphere microorganisms on plant growth and root development. High requirements for experimental equipment to achieve the effect of promoting plant growth and root development and reducing the impact of CO2 deficiency

Pending Publication Date: 2020-12-29
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, liquid chromatographic detection, which is usually used to identify whether bacteria can synthesize auxin, has high requirements for experimental equipment, and it is difficult to operate in ordinary laboratories. The experimental operation is cumbersome and expensive.
After searching, there are few reports about using the experimental system to detect the effect of rhizosphere microorganisms on plant growth and root system development to determine whether rhizosphere microorganisms can promote plant growth and root system development, and to determine whether rhizosphere microorganisms can synthesize auxin

Method used

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  • Experimental system for detecting influence of rhizosphere microorganisms on plant growth and root development and application thereof
  • Experimental system for detecting influence of rhizosphere microorganisms on plant growth and root development and application thereof
  • Experimental system for detecting influence of rhizosphere microorganisms on plant growth and root development and application thereof

Examples

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Effect test

Embodiment 1

[0021] Example 1: Use the experimental system of the present invention to verify the effects of bacteria including rhizosphere probiotics and pathogenic bacteria on plant growth and lateral root development.

[0022] In order to exclude CO 2 and other volatile substances, to study the influence of rhizosphere bacteria on plant growth or lateral root development, the present invention has designed a kind of experimental system for detecting the influence of rhizosphere microorganisms on plant growth and root system development on the basis of previous experiments: The two-compartment petri dish is an experimental mold, and the sterile Arabidopsis wild-type (Col-0) seedlings that have grown for 5 ± 1 days are transferred to the 1 / 2MS solid medium without sucrose in the two-compartment petri dish, wherein The roots of one grid of seedlings are directly inoculated with rhizosphere bacterial liquid, and the seedlings of the other grid are used as a control. No bacteria are inoculat...

Embodiment 2

[0036] Example 2: Using the experimental system of the present invention to identify whether microorganisms have a tryptophan-dependent auxin synthesis pathway.

[0037] Many rhizobacteria have tryptophan-dependent auxin synthesis pathways, which can synthesize auxin, promote the development of plant roots, and then promote the absorption of water and nutrients in the soil by plants. In order to verify whether the experimental system of the present invention can determine whether a certain bacterium has an auxin synthesis pathway and whether it can synthesize auxin, this example uses the experimental system to detect whether the rhizosphere probiotic S.marcescens can synthesize auxin .

[0038] The method is the same as in Example 1, and the transgenic Arabidopsis seedlings DR5::GUS grown for 5 days are transferred to 1 / 2 MS solid medium containing 50 μM Trp, and the rhizosphere probiotic S.marcescens is inoculated according to the method described in Example 1 After co-cultu...

Embodiment 3

[0044] Embodiment 3: An experimental system for detecting the influence of rhizosphere microorganisms on plant growth and root development.

[0045] The above-mentioned experimental system uses a two-compartment petri dish as an experimental mold, and the sterile Arabidopsis seedlings grown for 5 ± 1 days are transferred to the medium in the two-compartment petri dish ( figure 1 ), the root system of one grid of seedlings was directly inoculated with rhizosphere bacterial liquid, and the seedling of the other grid was used as a control, which was not inoculated but added with sterile water equal to the amount of bacterial liquid; Seal it with sensitive tape, place it vertically in a light incubator at 22±1°C, and cultivate it for 4-6 days under the condition that the photoperiod is 16 hours of light / 8 hours of darkness; by observing and measuring the seedlings of the aboveground part of the plant, the length of the main root, Count the density of lateral roots to judge whether...

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Abstract

The invention discloses an experimental system for detecting the influence of rhizosphere microorganisms on plant growth and root development. The experimental system is characterized in that a two-grid culture dish is used as an experimental mold, sterile arabidopsis seedlings growing for 5 + / -1 days are transferred to a culture medium in the dish, rhizosphere bacterial liquid is directly inoculated on the root system of one grid of seedlings, the other grid of seedlings is used as a contrast, and sterile water of which the amount is equal to that of the bacterial liquid is added without inoculating bacteria; then the two-grid culture dish is sealed with a breathable pressure-sensitive adhesive tape, and is vertically placed in an illumination incubator, and culturing is performed for 4-6days; and whether inoculated microorganisms can promote plant growth and root system development or not is judged by observing and measuring the lengths of seedlings and main roots of the overgroundparts of plants and counting the density of lateral roots, or whether the microorganisms have tryptophan-dependent auxin synthetic pathways or not is identified. The system is helpful for screening and application of rhizosphere microorganisms for promoting plant growth and root development, and has important value for agricultural production and microorganism exploration.

Description

technical field [0001] The invention relates to an experimental system for studying the interaction between plants and rhizosphere microorganisms, in particular to an experimental system for detecting the influence of rhizosphere microorganisms on plant growth and root system development and its application. It belongs to the field of agricultural production technology and application. Background technique [0002] The rhizosphere refers to the micro-domain soil area around the root system of plants and affected by the root system. The roots of plants can secrete a large amount of sugars, amino acids, organic acids and other organic substances around the soil (Badri and Vivanco 2009), and these organic substances can recruit and promote the growth and reproduction of microorganisms in the rhizosphere of plants (Dakora and Phillips 2002; Dennis, Miller et al. 2010). Among them, rhizosphere bacteria are the main components of plant rhizosphere microorganisms. Studies have fo...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/04
CPCA01H1/04A01H4/001A01H4/008
Inventor 丁兆军张春雷张蒙悦
Owner SHANDONG UNIV
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