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Markers used for diagnosis of delayed preeclampsia

A preeclampsia and marker technology, applied in the field of markers for the diagnosis of delayed preeclampsia, can solve the problems of low sensitivity and less research, and achieve the effect of convenient material collection and avoiding medical waste.

Pending Publication Date: 2020-12-25
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This diagnostic method can minimize the missed diagnosis for patients with suspected preeclampsia, but its disadvantage is that the sensitivity is not high, and continuous monitoring is often required to make a diagnosis
Therefore, differentially expressed genes in the peripheral blood of late-onset preeclampsia pregnant women and normal pregnant women is a good potential method for screening late-onset preeclampsia markers, but there are few related studies in the prior art

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  • Markers used for diagnosis of delayed preeclampsia
  • Markers used for diagnosis of delayed preeclampsia
  • Markers used for diagnosis of delayed preeclampsia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Screening of novel diagnostic markers

[0053] Search a preeclampsia peripheral blood blood cell expression microarray database in Gene Expression Omnibus (GEO) (GSE48424) [16] , respectively compare different types of preeclampsia with the corresponding control group to screen differentially expressed genes ((Fold Change≥2; P-valuefigure 1 , the number of differentially expressed genes in early-onset preeclampsia was significantly lower than that in late-onset preeclampsia, and there was also heterogeneity among late-onset preeclampsia with different severity, such as figure 2 .

[0054] In order to further screen the genes that play a pivotal role in the onset of preeclampsia, we screened the genes in the microarray data for highly variable genes and performed correlation analysis, and divided them into 18 different modules, such as image 3 , there are mainly salmon, red, yellow, green, magenta, turquoise, black, blue and other modules.

[0055] Through...

Embodiment 2

[0058] Example 2: Detection of markers

[0059] 1) Take 2ml of venous blood with EDTA anticoagulant tube, and mix it upside down.

[0060] 2) Divide 2mL of peripheral blood into two 2mL EP tubes, 1mL each, 1500×g, 5min, 4°C;

[0061] 3) Add 1mL of PBS to the remaining blood cells, mix well to wash the cells, 1500×g, 5min, 4°C;

[0062] 4) Discard the supernatant, add 1 mL of TRIZOL (Invitrogen, 15596026) to the remaining blood cells, and mix well;

[0063] 5) After inverting and mixing, place it at room temperature for 10 minutes, add 200ul chloroform, vortex and mix thoroughly, and place it on ice for 5 minutes; 15000×r, 15 minutes, 4°C;

[0064] 6) Carefully pipette the supernatant into a new 1.5mL EP tube, add an equal amount of isopropanol to mix, and place at -20°C for 30min+;

[0065] 7) 15000×r, 5min, 4°C, pour the supernatant, add 1mL of pre-cooled 75% ethanol, blow and mix well;

[0066] 8) 15000×r, 5min, 4°C, repeat step 7 once;

[0067] 9) Pour the supernatant,...

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Abstract

The invention discloses a group of markers used for diagnosis of delayed preeclampsia. According to the invention, differential expression genes of peripheral blood cells of a delayedpreeclampsia patient and a normal pregnant woman are screened on the basis of a gene chip technology, hub genes in disease occurrence are analyzed and screened through a co-expression network, and diagnostic values ofthe markers in practical application are verified through fluorescent quantitative PCR. The markers are high in specificity, good help can be provided for differential diagnosis of the delayed preeclampsia, meanwhile, the method has the advantages of non-invasiveness and simplicity for detecting specimen, and a new strategy can be provided for general screening of clinical suspected delayed preeclampsia pregnant women.

Description

technical field [0001] The present invention relates to a group of markers for the diagnosis of delayed preeclampsia. Background technique [0002] Preeclampsia (preeclampsia) refers to the new onset of hypertension, proteinuria or other end-organ damage in pregnant women after 20 weeks of pregnancy. The incidence rate is 4-5%. 500,000 pregnant women and 500,000 fetuses died due to [1] . Preeclampsia is a dynamic disease with continuous progression, which can be divided into early-onset (≤34 weeks, early-onset of preeclampsia; EOPE) and late-onset (>34 weeks, late -onsetofpreeclampsia; LOPE) two types of preeclampsia. The jury is still out on whether the two types of preeclampsia are considered to be one disease spanning a longer gestational period or two distinct disorders, but a growing body of research suggests that the pathogenesis of the two preeclampsia is quite different: early onset eclampsia Preeclampsia is associated with developmental defects of the placent...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/136C12Q2600/158
Inventor 张宏波王帅玉张宝杨浩
Owner SUN YAT SEN UNIV
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