Modeling method for mice with diseases of depression and liver-stagnation and spleen-deficiency syndrome

Abstract

The invention relates to the technical field of depression research, in particular to a modeling method for mice with diseases of depression and liver-stagnation and spleen-deficiency syndrome. Healthy mice are continuously stimulated for 28 days, each type of stimulation is averagely used for 4 times, and the stimulation types are as follows: water forbidding and food interruption stimulation for24 hours, tail clamping stimulation for 1 min, ice water swimming stimulation at 10 DEG C for 1 min, horizontal shaking continuous stimulation at the frequency of 1 time / s for 5 min, lamplight irradiating stimulation for 24 h, saturated water wet padding stimulation for 24 h or continuous stimulation by inclining a mouse cage by 45 degrees for 24 h; from the fifteenth day to the 28th day of continuous stimulation, 0.5 mL of rheum officinale powder aqueous suspension with the concentration of 15% (wt) is further perfused into stomachs of the mice every day. The obtained model mice have the symptomatic expression of depression and liver-stagnation and spleen-deficiency syndrome, and can be used for further research on pathogenesis and treatment of depression and liver-stagnation and spleen-deficiency syndrome.

Description

technical field [0001] The invention relates to the technical field of depression research, in particular to the animal modeling technology of the disease. Background technique [0002] Depression is a serious emotional disorder. The survey found that the prevalence of depression is as high as 10% worldwide, and its lifetime prevalence is as high as 20%. The suicide rate of depression is high, and more than half of Depression patients once had the idea of ​​"suicide", and the number of suicide deaths due to depression exceeds 1 million every year. The high morbidity, disability and suicide rates have serious impacts on families and society. Moreover, the incidence of depression is increasing year by year. [0003] Depression treatment does not respond well. Less than 50% of patients benefit from standard antidepressant treatment, and about 70% of patients still have some residual symptoms. This may be due to the fact that depression is a highly heterogeneous disease, and...

AI Technical Summary

Problems solved by technology

At present, most of the animal models of different TCM syndrome types of depression are based on the modeling method of depression, that is, the "disease" modeling method of Western medicine. They often summarize the TCM syndrome types through observation or reverse the possible syndrome types according to the treatment effect of TCM. Lag and lack of specificity lead to inconsistency and uncertainty in research results, affecting clinical translation of basic research

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