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Pepsinogen I/II determination kit and preparation method thereof

A pepsinogen and kit technology, applied in the field of immunodetection, can solve the problems of complex operation process, many influencing factors, poor precision, etc.

Active Publication Date: 2020-12-18
WUHAN LIFE ORIGIN BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzyme-linked immunosorbent assay method is complex in operation, takes a long time to measure, has many influencing factors, and is not suitable for the detection of emergency samples; immunochromatography has the advantage of short test time, but is limited by material factors and other reasons, and the precision is poor It is its biggest disadvantage; when the amount of antigen or antibody is too large, soluble complexes may appear in the determination of immunoturbidimetric method, which will cause errors and be easily affected by lipemia
Although chemiluminescence immunoassay has many advantages such as short reaction time and low sensitivity, but the anti-interference performance is low, and because it is a liquid reagent, the stability and precision are greatly affected by the diluent

Method used

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  • Pepsinogen I/II determination kit and preparation method thereof
  • Pepsinogen I/II determination kit and preparation method thereof
  • Pepsinogen I/II determination kit and preparation method thereof

Examples

Experimental program
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Embodiment approach

[0038] According to a typical embodiment of the present invention, a pepsinogen I / II assay kit is provided, the kit comprising: reagent M and reagent R;

[0039] The reagent M is a working solution obtained by diluting magnetic particles coated with anti-pepsinogen I / II antibody with a magnetic bead diluent; wherein, the formula of the magnetic bead diluent is:

[0040] HEPES buffer 0.04~0.06mol / L, protein 9~11g / L, protective agent 1~4g / L, sucrose 29~31g / L, S17 (Rhodasur ON-870) 1.5~2.5g / L, preservative 0.5~ 1.5g / L, pH 7.0-7.5;

[0041] The reagent R is a working solution obtained by diluting the alkaline phosphatase-labeled anti-pepsinogen I / II antibody with an enzyme-labeled diluent; wherein, the formula of the enzyme-labeled diluent is:

[0042] MES buffer 0.04~0.06mol / L, casein 19~21g / L, sucrose 39~41g / L, dispersant 1~5g / L, blocking agent 0.05~0.15g / L, ZnCl 2 0.13~0.14g / L, MgCl 2 1~1.5g / L, Tween 20 1.5~2.5g / L, preservative 0.5~1.5g / L, NaCl 8~9g / L, pH 6.0-6.5.

[0043...

Embodiment 2

[0112] On the basis of Example 1, the gelatin in the magnetic bead dilution was replaced with alginic acid, and other components and processes were the same as in Example 1.

Embodiment 3

[0114] On the basis of Example 1, the gelatin in the magnetic bead dilution was replaced with pectin, and other components and processes were the same as in Example 1.

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Abstract

The invention provides a pepsinogen I / II determination kit and a preparation method thereof. A magnetic bead diluent in the kit comprises 0.04-0.06 mol / L of HEPES, 9-11 g / L of protein, 1-4 g / L of a protective agent, 29-31 g / L of sucrose, 1.5-2.5 g / L of S17 and 0.5-1.5 g / L of a preservative, and the pH value is 7.07.5. The enzyme-labeled diluent comprises 0.04 to 0.06 mol / L of MES, 19 to 21 g / L ofcasein, 39 to 41 g / L of sucrose, 1 to 5 g / L of a dispersant, 0.05 to 0.15 g / L of a blocking agent, 0.13 to 0.14 g / L of ZnCl2, 1 to 1.5 g / L of MgCl2, 1.5 to 2.5 g / L of Tween 20, 0.5 to 1.5 g / L of a preservative and 8 to 9 g / L of NaCl, the PH value is 6.0-6.5; and the stability is high.

Description

technical field [0001] The invention belongs to the technical field of immunoassay, and relates to a pepsinogen I / II assay kit and a preparation method thereof. Background technique [0002] Pepsinogen is the inactive precursor of pepsin. It is divided into 2 subgroups according to its biochemical properties and immunogenicity. Components 1-5 have the same immunogenicity and are called pepsinogen I. It is mainly composed of gastric fundus Secreted by chief cells and mucous neck cells of the gland; components 6 and 7 are called pepsinogen II, in addition to being secreted by chief cells and mucous neck cells of the fundic glands, mucous neck cells of the pyloric glands of the cardia glands and antrum And the upper duodenum can also produce pepsinogen II. Most of the PG synthesized in the stomach enters the gastric cavity and is activated into pepsin under the action of acidic gastric juice. Only a small amount of PG (about 1%) enters the blood circulation through the capilla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/573G01N33/543
CPCG01N33/581G01N33/573G01N33/54326G01N33/54333G01N2333/96477Y02A50/30
Inventor 常浩来祥兵
Owner WUHAN LIFE ORIGIN BIOTECH LTD
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