Polygene methylation detection kit for nasopharyngeal carcinoma diagnosis

Abstract

The invention belongs to the field of molecular diagnosis, and particularly relates to a polygene methylation detection kit for the nasopharyngeal carcinoma diagnosis. The kit comprises a p16 gene extraction reagent group, a p16 gene methylation modification reagent group, a fluorescent quantitative PCR detection reagent group and a p16 gene methylation level calibrator. Compared with the prior art, the kit has the beneficial effects that the p16 gene in peripheral blood of a patient is extracted through superparamagnetic beads, the detection is carried out through fluorescent quantitative PCR, a detection sample is easier to obtain, the DNA extraction is simpler, more convenient and faster, and the commercialized application and popularization of the kit are facilitated; in addition, methylation detection primers adopted by the kit can more efficiently detect the methylation level of the p16 gene; and moreover, the p16 gene methylation level calibrator in the kit can ensure the reliability of a detection result.

Description

technical field [0001] The invention belongs to the field of molecular diagnosis, and in particular relates to a multi-gene methylation detection kit for nasopharyngeal carcinoma diagnosis. Background technique [0002] my country is one of the countries with a high incidence of nasopharyngeal carcinoma (tumor). The incidence rate in the south is higher than that in the north, especially in Guangxi, Guangdong, Fujian, Hunan and other provinces. It is difficult to find nasopharyngeal carcinoma by yourself. Even if some symptoms are found and go to the hospital for examination, 80% of them are already in the advanced stage. Due to the concealed position of the nasopharynx, it is not easy to check. At the same time, the early symptoms of nasopharyngeal carcinoma are complex and lack of characteristics, so they are easily overlooked and delay diagnosis and treatment. Therefore, vigilance must be increased. [0003] Methylation refers to the process of catalytic transfer of meth...

AI Technical Summary

Problems solved by technology

[0004] The abnormal methylation level of the p16 gene is of great significance for the detection and prognosis of nasopharyngeal carcinoma in high-risk groups, but the current detection process for the abnormal methylation level of the p16 gene is complicated, time-consuming and costly