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Culture method of hair follicle mesenchymal stem cells

A technology of mesenchymal stem cells and culture methods, which is applied in the field of hair follicle mesenchymal stem cell culture, can solve the problems such as the source limitation of autologous mesenchymal stem cells, and achieve the effects of convenient material collection, improved quality, and improved expansion ability

Pending Publication Date: 2020-12-11
YINFENG JILIN BIOLOGICAL ENG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to overcome the deficiencies in the prior art, the present invention provides a method for culturing hair follicle mesenchymal stem cells, so as to solve the current technical problem of the limitation of the source of autologous mesenchymal stem cells

Method used

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  • Culture method of hair follicle mesenchymal stem cells
  • Culture method of hair follicle mesenchymal stem cells
  • Culture method of hair follicle mesenchymal stem cells

Examples

Experimental program
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Embodiment 1

[0050] Embodiment 1 A kind of culture method of hair follicle mesenchymal stem cells

[0051] (1) Isolation and culture of primary hair follicle mesenchymal stem cells

[0052] 1) Preparation of complete medium: serum-free medium + 5% serum substitute + 20ng / ml epidermal growth factor + 20ng / ml fibroblast growth factor + 20ng / ml platelet-derived growth factor + 20ng / ml vascular endothelial growth factor.

[0053] 2) 20 human hairs with intact hair follicles were directly extracted, and the centrifuge tube was placed in an ice box, placed in a centrifuge tube containing 0.1% gentamicin and physiological saline, and transported to the laboratory.

[0054] 3) Cut off the hair, rinse three times with normal saline containing 0.1% gentamicin, and then rinse three times with normal saline.

[0055] 4) Tissue shears are mechanically sheared.

[0056] 5) The chopped tissue was resuspended in physiological saline, centrifuged at 1500 rpm for 5 min, and the supernatant was discarded;...

Embodiment 2- Embodiment 19

[0061] Example 2-Example 19 A method for culturing hair follicle mesenchymal stem cells

[0062] Same as Example 1.

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Abstract

The present invention relates to a culture method of hair follicle mesenchymal stem cells and belongs to the field of biology. The culture method of the hair follicle mesenchymal stem cells comprisesthe following steps: step 1, acquiring primary hair follicle tissue blocks; step 2, inoculating the obtained primary hair follicle tissue blocks into a cell culture medium for a culture treatment; andstep 3, carrying out biological identification on amplified cells, wherein results show that (1) the cells are adhered to walls; (2) cell phenotypes are as follows: CD44+ is greater than or equal to95%, CD105+ is greater than or equal to 95%, CD45+ is less than or equal to 2% and CD34+ is less than or equal to 2%; and (3) the cells can be differentiated into adipocytes, osteocytes and chondrocytes. The culture method can effectively improve proliferation of the hair follicle mesenchymal stem cells, improves an amplification capacity of the hair follicle mesenchymal stem cells in a culture process, is convenient to obtain materials, has small pain to obtain the materials, and is rich in sources and simple in separation.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for culturing hair follicle mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (Mesenchymal Stem Cells, MSCs) were first discovered in the bone marrow and have been confirmed to exist in various tissues and organs of the body. They are a group of pluripotent stem cells with multi-directional differentiation potential. [0003] The mesenchymal stem cells of dental pulp, adipose, and peripheral blood may have problems with low cell numbers, or have invasive problems, or have problems limited by physical health, so that the application of autologous mesenchymal stem cells cannot be realized. Therefore, it is very necessary to find new mesenchymal stem cells and cultivate them successfully. SUMMARY OF THE INVENTION [0004] In order to overcome the deficiencies in the prior art, the present invention provides a method for culturing hair follicle mese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0666C12N2500/90C12N2501/11C12N2501/135C12N2501/165
Inventor 单延红李珈莹郭华于沛勇张建中刘潇潇
Owner YINFENG JILIN BIOLOGICAL ENG TECH CO LTD
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