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Porcine reproductive and respiratory syndrome inactivated vaccine immunopotentiator and preparation method thereof

An immune enhancer and respiratory syndrome technology, applied in the field of porcine reproductive and respiratory syndrome inactivated vaccine immune enhancer and its preparation, can solve the problem of large-scale promotion and use of inactivated vaccines, low antibody levels, mucosal antibodies, and antibody production Slow down and other problems, to achieve the effect of shortening the immune window period, increasing the antibody level, and improving the immune level

Active Publication Date: 2020-12-11
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the shortcomings of existing inactivated vaccines are obvious, mainly reflected in slow antibody production, short immune period, narrow antigen spectrum, low antibody level, and inability to induce mucosal antibodies; the shortcomings of these inactivated vaccines have seriously affected the inactivation The large-scale promotion and use of vaccines requires an immune enhancer that can improve the immune enhancement of antigens to make up for the defects in current inactivated vaccines

Method used

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  • Porcine reproductive and respiratory syndrome inactivated vaccine immunopotentiator and preparation method thereof

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Embodiment 1

[0027] This embodiment is the preparation method of the first porcine cytoplasmic polypeptide, comprising the following steps:

[0028] S1. Take fresh porcine tissue, wash it with deionized water, divide it, add deionized water and mince it at high speed to form a slurry;

[0029] S2. Add 1% of the total mass of sodium chloride to the slurry, heat to dissolve at 80° C., then perform suction filtration, collect the filtrate, and obtain the first filtrate and filter residue;

[0030] S3. Add 2709 alkaline protease of 2% of the total weight of the first filtrate and 1% of the porcine cytoplasmic polypeptide extractant of the total weight of the first filtrate to the first filtrate, and intermittently stir at a temperature of 55° C. and a pH of 8, Enzymolysis and extraction for 5 hours to obtain the first enzymolysis extraction solution; wherein the porcine cytoplasmic polypeptide extractant in this embodiment comprises 1 L of phosphoric acid solution, 2 L of acetonitrile solution...

Embodiment 2

[0035] This embodiment is the preparation method of the second porcine cytoplasmic polypeptide, comprising the following steps:

[0036] S1. Take fresh porcine tissue, wash it with deionized water, divide it, add deionized water and mince it at high speed to form a slurry;

[0037] S2. Add 1.5% of the total mass of sodium chloride to the slurry, heat to dissolve at 90° C., then perform suction filtration, collect the filtrate, and obtain the first filtrate and filter residue;

[0038] S3. Add 2709 alkaline protease of 2.5% of the total weight of the first filtrate and 1.5% of the porcine cytoplasmic polypeptide extractant to the first filtrate, and intermittently stir at a temperature of 60°C and a pH of 8.5, Enzymolysis and extraction for 6 hours to obtain the first enzymolysis extraction solution; wherein the porcine cytoplasmic polypeptide extractant in this embodiment includes 1.5 L of phosphoric acid solution, 2.5 L of acetonitrile solution, 0.65 L of methanol solution an...

Embodiment 3

[0043] This embodiment is the preparation method of the third porcine cytoplasmic polypeptide, comprising the following steps:

[0044] S1. Take fresh porcine tissue, wash it with deionized water, divide it, add deionized water and mince it at high speed to form a slurry;

[0045] S2. Add 2% of the total mass of sodium chloride to the slurry, heat to dissolve at 100° C., then perform suction filtration, collect the filtrate, and obtain the first filtrate and filter residue;

[0046] S3. Add 3% of the total weight of the first filtrate to the 2709 alkaline protease and 2% of the total weight of the first filtrate to the porcine cytoplasmic polypeptide extractant, and intermittently stir at a temperature of 65°C and a pH of 9, Enzymolysis and extraction for 7 hours to obtain the first enzymolysis extraction solution; wherein the porcine cytoplasmic polypeptide extractant in this embodiment includes 2 L of phosphoric acid solution, 3 L of acetonitrile solution, 0.8 L of methanol ...

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Abstract

The invention relates to the technical field of inactivated vaccines, and discloses a porcine reproductive and respiratory syndrome inactivated vaccine immunopotentiator and a preparation method thereof. The porcine reproductive and respiratory syndrome inactivated vaccine immunopotentiator comprises 0.15-1.2 mg / mL of monophosphoryl lipid A, 0.5-0.9 mg / mL of beta- glucan and 10-50mg / mL of porcinecytoplasm polypeptide; and the immunopotentiator comprises 6-12 parts of monophosphoryl lipid A, 7-11 parts of beta- glucan and 10-18 parts of porcine cytoplasm polypeptide in parts by mass. The preparation method comprises the following steps of mixing a mixed solution containing monophosphoryl lipid A, porcine cytoplasm polypeptide and beta-glucan with Tween-80 to obtain a water phase solution;then mixing white oil with span-80 to obtain an oil phase solution; and finally, mixing and emulsifying the water phase solution and the oil phase solution to obtain the inactivated vaccine immunopotentiator. The immune time of the porcine reproductive and respiratory syndrome inactivated vaccine can be prolonged, and the antibody level is improved.

Description

technical field [0001] The invention relates to the technical field of inactivated vaccines, in particular to an immune enhancer for porcine reproductive and respiratory syndrome inactivated vaccines and a preparation method thereof. Background technique [0002] Porcine reproductive and respiratory syndrome (abbreviated as PRRS), also known as porcine blue ear disease, is caused by porcine reproductive and respiratory syndrome virus (abbreviated as PRRSV). , a disease of piglets and finishing pigs with respiratory symptoms. It is difficult to effectively prevent and control PRRS. First, PRRSV targets lung macrophages and has antibody-dependent enhancement (ADE), causing certain immunosuppression. Secondly, PRRSV is constantly mutating, and there is no effective treatment for PRRS Drugs, currently, vaccine immunization is considered to be an important means to prevent and control PRRS. At the same time, compared with other control methods, vaccine immunization is also the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/12A61P31/14
CPCA61K39/39A61K39/12A61P31/14A61K2039/552A61K2039/55572A61K2039/55583A61K2039/55516A61K2039/5252C12N2770/10034
Inventor 沈建军陈雪张秀文屈健陈芳李阳
Owner 浙江美保龙生物技术有限公司
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