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Group of markers for predicting cytokines and thrombostorms of 2019 coronavirus, application and kit

A coronavirus and cytokine technology, applied in the field of biomedicine, can solve problems such as unclear research conclusions, and achieve the effects of broad application prospects, rapid detection, and low cost

Active Publication Date: 2020-12-04
BEIJING IMMUPEUTICS MEDICINE TECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the predictive markers of "thrombotic storm" at present, and the research conclusions are not yet clear.

Method used

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  • Group of markers for predicting cytokines and thrombostorms of 2019 coronavirus, application and kit
  • Group of markers for predicting cytokines and thrombostorms of 2019 coronavirus, application and kit
  • Group of markers for predicting cytokines and thrombostorms of 2019 coronavirus, application and kit

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Experimental program
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Effect test

preparation example Construction

[0033] In the present invention, the preparation method of the kit preferably comprises the following steps:

[0034] (1) the capture antibody coated with one or more than two of VEGF-D, TNF-α, SCF, LIF, IL-2, IL-4, IL-6, IL-8, IL-10, IL-15, IL-17A, IL-18, IL-1β, and IFN-γ, respectively, is mixed with Get the coded microspheres coated with one or more than two capture antibodies of VEGF-D, TNF-α, SCF, LIF, IL-2, IL-4, IL-6, IL-8, IL-10, IL-15, IL-17A, IL-18, IL-1β and IFN-γ;

[0035] (2) attaching biotin to one or more than two detection antibodies of VEGF-D, TNF-α, SCF, LIF, IL-2, IL-4, IL-6, IL-8, IL-10, IL-15, IL-17A, IL-18, IL-1β and IFN-γ, respectively. Get one or more than two detection antibodies of VEGF-D, TNF-α, SCF, LIF, IL-2, IL-4, IL-6, IL-8, IL-10, IL-17A, IL-18, IL-1β and IFN-γ respectively labeled with biotin;

[0036] There is no sequential relationship between the steps (1) and (2).

[0037] In the invention, one or more capture antibodies of VEGF-D, TNF-α, SCF, L...

Embodiment 1

[0062] Preparation of liquid-phase chip kit for detecting biomarkers of VEGF-D, TNF-α, SCF, LIF, IL-2, IL-4, IL-6, IL-8, IL-10, IL-15, IL-17A, IL-18, IL-1β and IFN-γ.

[0063] 1. Composition of kit

[0064] (1)14-plex coated microspheres: containing VEGF-D, TNF-α, SCF, LIF, IL-2, IL-4, IL-6, IL-8, IL-10, IL-15, IL-17A, IL-18, IL-1β and IFN-γ respectively.

[0065] (2)14-plex biotin-labeled antibody: VEGF-D, TNF-α, SCF, LIF, IL-2, IL-4, IL-6, IL-8, IL-10, IL-15, IL-17A, IL-18, IL-1β and IFN-respectively labeled with biotin.

[0066] (3) streptavidin phycoerythrin.

[0067] The clone numbers of capture antibodies VEGF-D, TNF-α, SCF, LIF, IL-2, IL-4, IL-6, IL-8, IL-10, IL-15, IL-17A, IL-18, IL-1β and IFN-γ are 106 and 28401, respectively. The clone numbers of VEGF-D, TNF-α, SCF, IL-4, IL-6, IL-8, IL-17A, IL-1β and IFN-γ antibodies are OTI1C7, 52B83, SC61-06, 25D2, 5IL6, 3IL8-H10, respectively.

[0068] 2. Preparation method of kit

[0069] Comprises the following steps:

[0070] (1) ...

Embodiment 2

[0089] The application of VEGF-D, TNF-α, SCF, LIF, IL-2, IL-4, IL-6, IL-8, IL-10, IL-15, IL-17A, IL-18, IL-1β and IFN-γ liquid chip kits in predicting COVID-19 cytokine release syndrome and blood clot storm

[0090] 1, experimental purpose

[0091] It is proved that the baseline concentrations of VEGF-D, TNF-α, SCF, LIF, IL-2, IL-4, IL-6, IL-8, IL-10, IL-15, IL-17A, IL-18, IL-1β and IFN-γ at admission are in Covid-γ where cytokine release syndrome and blood clot storm occur.

[0092] 2. Experimental subjects

[0093] 1) On the premise of informed consent and meeting the inclusion conditions, select the enrolled cases and record the basic personal information. The COVID-19 patient cohort came from 24 COVID patients in beijing ditan hospital affiliated to Capital Medical University, including 14 critically ill patients and 10 critically ill patients. .

[0094] 2) Extract the untreated plasma of the patient at the time of admission, and store the specimen in the refrigerator at -80℃...

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Abstract

The invention provides a group of markers for predicting cytokine release syndrome and thrombostorm of 2019 coronavirus, application of the markers, a kit and a preparation method of the kit, and belongs to the technical field of biological medicines. The markers provided by the invention are respectively VEGFD, TNF-alpha, SCF, LIF, IL-2, IL-4, IL-6, IL-8, IL-10, IL-15 , IL-17A, IL-18, IL-1beta and IFN-gamma. The kit for predicting the cytokine release syndrome and thrombus storm of the 2019 coronavirus disease (COVED19) by utilizing the group of markers of VEGFD, TNF-alpha, SCF, LIF, IL-2, IL-4, IL-6, IL-8, IL-10, IL-15 , IL-17A, IL-18, IL-1beta and IFN-gamma or the kit prepared from the markers has the advantages of rapid and accurate detection, low cost and the like.

Description

Technical field [0001] The invention belongs to the technical field of biomedicine, and particularly relates to a group of markers for predicting cytokine release syndrome and thrombus storm of coronavirus disease (COVID-19) in 2019, applications thereof, a kit and a preparation method of the kit. technical background [0002] Among the 2019 patients with coronavirus disease (COVID-19), about 15% of COVID-19 patients developed severe cases, and 5% to 6% of them developed critical cases. The mortality of COVID-19 critically ill patients is significantly higher than that of critically ill patients. It is reported that "cytokine release syndrome and thrombotic storm" is related to the disease criticality and lethality of COVID-19 patients. Therefore, it is urgent to develop markers that can effectively predict "cytokine release syndrome and thrombus storm" at an early stage to distinguish critical patients from critical patients, so as to formulate effective treatment plans in advan...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/68G01N33/569G01N33/543
CPCG01N33/74G01N33/6863G01N33/6869G01N33/6866G01N33/56983G01N33/54313
Inventor 沈宁陈莉陈衍辉王修芳宋瑾
Owner BEIJING IMMUPEUTICS MEDICINE TECH LTD
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