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Method for catalytically synthesizing phytosterol ester compound by using magnetic immobilized lipase

A technology for immobilizing lipase and phytosterol esters, applied in biochemical equipment and methods, fixed on or in inorganic carriers, fixed on/in organic carriers, etc., can solve the problem of lipase reusability High, low tolerance to organic solvents, etc., to achieve good physical and chemical stability, improve catalytic activity, and high catalytic activity

Active Publication Date: 2020-12-04
NANJING UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2019, Zhou Rui et al. used chitosan-glutaraldehyde as a carrier to immobilize Candida rugosa lipase. Although the stability of the lipase has been improved, the reusability of the lipase is not high. Only about 20% of the initial enzyme activity is retained, and the tolerance in organic solvents is not high. After soaking in n-heptanol for 120 days, the enzyme activity of the immobilized enzyme only retains 32.2% (Zhou Rui, Xing Yanhua, Wang Yan .Using chitosan-glutaraldehyde as a carrier to flexibly immobilize Candida rugosa lipase[J].Modern Chemical Industry, 2019, 48(8):13.)

Method used

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  • Method for catalytically synthesizing phytosterol ester compound by using magnetic immobilized lipase
  • Method for catalytically synthesizing phytosterol ester compound by using magnetic immobilized lipase
  • Method for catalytically synthesizing phytosterol ester compound by using magnetic immobilized lipase

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Embodiment 1

[0044] (1) Co-precipitation method to synthesize magnetic nano ferric oxide particles, dissolve 4.5g of ferric chloride hexahydrate and 6g of ferrous chloride tetrahydrate in 90ml of deionized water, fully shake and mix evenly at 60°C and 150r / min Finally, add a certain amount of 2mol / L NaOH solution, adjust the pH to 10, continue to stir for 1h, then perform magnetic separation, wash with deionized water until neutral to obtain black solid-magnetic nanometer ferric oxide particles.

[0045] (2) Add 2 g of chitosan into 100 mL of acetic acid solution and stir well to obtain a uniform chitosan solution. The Fe obtained in (1) 3 o 4 Add magnetic nanoparticles into the solution, add NaOH solution after ultrasonication for 0.5h to adjust the pH to 7±0.5, wash with deionized water after magnetic separation and dry to obtain chitosan-wrapped magnetic nano iron ferric oxide particles.

[0046] (3) Dissolve 1.5 g of dialdehyde cellulose into 50 mL of deionized water, and stir well t...

Embodiment 2

[0050] (1) Co-precipitation method to synthesize magnetic nano ferric oxide particles, dissolve 5g of ferric chloride hexahydrate and 6.7g of ferrous chloride tetrahydrate in 100ml of deionized water, fully shake and mix evenly at 60°C and 150r / min Finally, add a certain amount of 2mol / L NaOH solution, adjust the pH to 10, continue to stir for 1H, then carry out magnetic separation, wash with deionized water until neutral to obtain black solid-magnetic nanometer ferric oxide particles.

[0051] (2) Add 4 g of chitosan into 120 mL of acetic acid solution and stir well to obtain a uniform chitosan solution. The Fe obtained in (1) 3 o 4 Add magnetic nanoparticles into the solution, add NaOH solution after ultrasonication for 0.5h to adjust the pH to 7±0.5, wash with deionized water after magnetic separation and dry to obtain chitosan-wrapped magnetic nano iron ferric oxide particles.

[0052] (3) Dissolve 2 g of dialdehyde cellulose into 50 mL of deionized water, and stir well ...

Embodiment 3

[0056] (1) Co-precipitation method to synthesize magnetic nano ferric oxide particles, dissolve 5.3g ferric chloride hexahydrate and 7g ferrous chloride tetrahydrate in 100ml deionized water, fully shake and mix evenly at 60°C and 150r / min Finally, add a certain amount of 2mol / L NaOH solution, adjust the pH to 10, continue to stir for 1H, then carry out magnetic separation, wash with deionized water until neutral to obtain black solid-magnetic nanometer ferric oxide particles.

[0057] (2) Add 4 g of chitosan into 150 mL of acetic acid solution and stir thoroughly to obtain a uniform chitosan solution. The Fe obtained in (1) 3 o 4 Add magnetic nanoparticles into the solution, add NaOH solution after ultrasonication for 0.5h to adjust the pH to 7±0.5, wash with deionized water after magnetic separation and dry to obtain chitosan-wrapped magnetic nano iron ferric oxide particles.

[0058] (3) Dissolve 3 g of dialdehyde cellulose into 50 mL of deionized water, and stir well to ...

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Abstract

The invention provides a method for preparing phytosterol ester from a nano composite magnetic material-immobilized lipase. Chitosan with a polysaccharide chain structure and rich functional groups wraps an outer surface of a magnetic nano material ferroferric oxide, and then dialdehyde cellulose with an excellent flexible structure and biocompatibility is used as macromolecule cross-linked molecules to covalently immobilize lipase activated by an ionic liquid type surfactant. The organic-inorganic magnetic nano composite material immobilized enzyme prepared by the method has characteristics of high catalytic activity, convenience in separation, good reusability and the like, and can be used for catalyzing esterification reaction of phytosterol and fatty acid to prepare phytosterol ester.The invention provides a green and efficient new method for synthesizing phytosterol ester.

Description

technical field [0001] The invention belongs to the technical field of biocatalysis, and in particular relates to a method for synthesizing phytosterol ester by catalyzing the esterification reaction of phytosterol and fatty acid with magnetically immobilized lipase in an organic phase. Background technique [0002] Phytosterol is a natural compound with cyclopentane perhydrophenanthrene as the skeleton, which has the characteristics of antioxidant activity, treatment of osteoarthritis, and reduction of low-density lipoprotein cholesterol absorption. Phytosterols are poor in water solubility and fat solubility, and their absorption and utilization rate is low. The absorption and utilization rate of phytosterols after esterification is about 5 times higher than that of free phytosterols, which can significantly prevent hyperlipidemia and arteriosclerosis in mice. Modulating the content of small molecule metabolites of phospholipids and bile acids to improve the occurrence of ...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/12C12N11/10C12P33/00
CPCC12N11/14C12N11/12C12N11/10C12N9/20C12P33/00C12Y301/01003
Inventor 胡燚王舒舒吴正章张鹏邱香徐华金
Owner NANJING UNIV OF TECH
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