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Construction method and application of two-step decay model of nitrite oxidizing bacteria

A technology for oxidizing bacteria and nitrous acid, applied in chemical instruments and methods, biological treatment adjustment methods, special data processing applications, etc., can solve problems such as the inability to transform dead cells into soluble evaluations, and achieve improved sewage treatment effects and perfect models Effect

Inactive Publication Date: 2020-12-01
SHANDONG JIANZHU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the traditional bacterial decay model is only applicable to the primary stage of the decay stage of nitrite oxidizing bacteria (living cells→dead cells), and cannot evaluate the transformation of dead cells into soluble COD.

Method used

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  • Construction method and application of two-step decay model of nitrite oxidizing bacteria
  • Construction method and application of two-step decay model of nitrite oxidizing bacteria
  • Construction method and application of two-step decay model of nitrite oxidizing bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1 Construction of the two-step decay model of nitrous acid oxidizing bacteria of the present invention

[0033] 1. Use NaNO 2 (500mg-N / L), NH 4 CI(1.0mg-N / L), KH 2 PO4(0.13mg-P / L) and Na 2 Synthetic wastewater of PO4 (1.67-P / L) was enriched in sludge in a 5-L sequencing batch reactor (SBR) (the purpose of NOB enrichment is to eliminate the interference of other bacterial species). The pH, temperature and DO of the reactor were controlled at 7.3, 35±0.5°C and above 5mg / L, respectively. The SRT of the SBR reactor was maintained at 50 days, and after 240 days of cultivation, the experiment was carried out with concentrated NOB sludge.

[0034] 2. Concentrated NOB sludge uses NH 4 CI(1.0mg-N / L), KH 2 PO4(0.13mg-P / L) and Na 2 Synthetic wastewater of PO4 (1.67-P / L) was centrifuged three times (5000rpm), three minutes each time. Before NOB sludge cultivation, the sludge samples were centrifuged three times using nitrite-free inorganic synthetic wastewater to...

Embodiment 2

[0055] Example 2 Under the condition that the concentration of nitrous acid is 50 mg-N / L, the model equation established in Example 1 is used to detect and simulate OUR and COD of NOB.

[0056] (1) with NaNO 2 (500mg-N / L), NH 4 CI(1.0mg-N / L), KH 2 PO 4 (0.13mg-P / L) and Na 2 PO 4 (1.67-P / L) synthetic wastewater was enriched in sludge in a 5-L sequencing batch reactor (SBR) (the purpose of NOB enrichment is to eliminate the interference of other bacterial species). The pH, temperature and DO of the reactor were controlled at 7.3, 35±0.5°C and above 5mg / L, respectively. The SRT of the SBR reactor was maintained at 50 days, and after 240 days of cultivation, the experiment was carried out with concentrated NOB sludge.

[0057] (2) Concentrated NOB sludge with NH 4 CI(1.0mg-N / L), KH 2 PO 4 (0.13mg-P / L) and Na 2 PO 4 (1.67-P / L) synthetic wastewater was centrifuged three times (5000rpm), each time for three minutes. Before NOB sludge cultivation, the sludge samples were c...

Embodiment 3

[0069] Example 3 Under the condition that the concentration of nitrous acid is 2000 mg-N / L, the model equation established in Example 1 is used to detect and simulate OUR and COD of NOB.

[0070] (1) with NaNO 2 (500mg-N / L), NH 4 CI(1.0mg-N / L), KH 2 PO 4 (0.13mg-P / L) and Na 2 PO 4 (1.67-P / L) synthetic wastewater was enriched in sludge in a 5-L sequencing batch reactor (SBR). The pH, temperature and DO of the reactor were controlled at 7.3, 35±0.5°C and above 5mg / L, respectively. The SRT of the SBR reactor was maintained at 50 days, and after 240 days of cultivation, the experiment was carried out with concentrated NOB sludge.

[0071] (2) Concentrated NOB sludge with NH 4 CI(1.0mg-N / L), KH 2 PO 4 (0.13mg-P / L) and Na 2 PO 4 (1.67-P / L) synthetic wastewater was centrifuged three times (5000rpm), each time for three minutes.

[0072] (3) The NOB concentrated sludge without nitrite was used for the respiration test, and the NOB culture was carried out under the experime...

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Abstract

The invention relates to a construction method and application of a two-step decay model of nitrite oxidizing bacteria. The invention aims to solve the problem that a traditional bacterial decay modelis only suitable for a primary stage (living cells to dead cells) of an NOB decay stage and cannot evaluate the conversion of the dead cells into soluble COD. According to the method, the decay process of NOB under the condition of high-concentration nitrite is scientifically simulated, a two-step decay model (living cells to dead cells to soluble COD) is established, and model parameters of NOBdecay under nitrite inhibition are measured. According to the method, the two-step decay mathematical model of nitrite oxidizing bacteria is established for the first time, and short-cut nitrificationand denitrification nitrogen removal effect can be ensured by controlling the concentration of nitrite by utilizing the model; sludge treatment effect is improved; and technical support is provided for further understanding microorganism inhibition and activation recovery phenomena.

Description

technical field [0001] The invention belongs to the field of sewage treatment, and relates to a construction method and application of a two-step decay model of nitrite oxidizing bacteria, in particular to a two-step decay model of nitrite oxidizing bacteria suppressed by high nitrite concentration. Background technique [0002] The traditional biological denitrification process is that ammonia nitrogen generates nitrite under the action of ammonia oxidizing bacteria, and then nitrite is oxidized to nitrate by nitrite-oxidizing bacteria (NOB), and finally nitrate is denitrified by denitrifying bacteria. The process of generating nitrogen gas under action. The short-cut nitrification and denitrification process is to control the generation of nitrite, so that the denitrifying bacteria can directly use nitrite as the electron acceptor to complete the denitrification process. Compared with the traditional biological denitrification process, it has the obvious advantages of les...

Claims

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Application Information

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IPC IPC(8): C02F3/30G06F30/20C02F101/16
CPCC02F3/302C02F3/006G06F30/20C02F2101/16
Inventor 刘兵陈飞勇邵媛媛宋扬王静吴金柱李一凡徐保建杨寓超徐成远刘华
Owner SHANDONG JIANZHU UNIV
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