Chlamydia trachomatis antigen detection method and kit

Abstract

The invention provides a chlamydia trachomatis antigen detection method, which comprises the steps of: loading a colloidal gold labeled chlamydia trachomatis antibody on a solid nitrocellulose membrane, and coating the solid nitrocellulose membrane with a detection line prepared from an anti-chlamydia lipopolysaccharide monoclonal antibody-II and a control line prepared from an anti-mouse, anti-rabbit and anti-sheep IgG secondary antibody; mixing secreta of a human body with a 0.2 mol / L HCl solution; and putting the sample solution into a sample treatment tube containing a 0.2 mol / L NaOH solution, and dropwise adding the sample solution onto a solid nitrocellulose membrane, wherein when the solid nitrocellulose membrane displays a detection line and a control line at the same time, the secretion of the human body contains chlamydia trachomatis antigen, and the dispersing agent is prepared from a 0.2 mol / L NaOH solution and a 0.2 mol / L HCl solution which have the same volume. The chlamydia trachomatis antigen detection method provided by the invention is a one-step method, the specific high specificity rarely causes the problems of false negative or false positive and the like puzzling clinic, and the chlamydia trachomatis antigen detection method has the advantages of high sensitivity, simple operation and short time consumption.

Description

technical field [0001] The invention relates to the technical field of immunology, in particular to a chlamydia trachomatis antigen detection method and a kit. Background technique [0002] Chlamydia are divided into three groups according to their characteristics: Chlamydia Trachomatis, Chlamydia Psittas and Chlamydia Pneumoniac. There are 15 known serotypes of Chlamydia trachomatis, of which 12 serotypes are related to sand milk and reproductive tract infection; the other 3 are related to the occurrence of Lymphor Ganuloma Venereun (LGV). [0003] The method for diagnosing chlamydia infection in male and female patients is currently often using cell culture method. During the specific operation, the specimens of female cervical canal or male urethra are inoculated into a single cell layer, and then cultured for 48-72 hours, and the single cell layer is stained to Observe for typical inclusion bodies. If you need to improve the sensitivity of cell culture method, you can ...

AI Technical Summary

Problems solved by technology

If you need to improve the sensitivity of cell culture method, you can inoculate the negative culture into fresh monolayer cells, continue to culture for 48-72 hours and then observe. Although the cell culture method has high sensitivity and strong specificity, this method has a large workload and a long cycle , requires specialized equipment and personnel to operate, and the actual clinical application is limited

However, the recently established immunofluorescence method, synthetic enzyme chain reaction, and polymerase chain reaction detection all require skilled technicians to perform the test operation and test result interpretation, which requires high technical level of personnel and is inconvenient for direct interpretation of results.