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Method for establishing mouse lung cytomegalovirus latent reactivation infection model

A cytomegalovirus, a technology for establishing a method, applied in the field of establishing a model of pulmonary cytomegalovirus latent reactivation infection in mice, can solve the problem that the impact of HCMV body tissue cannot be comprehensively evaluated, and it is not suitable for pulmonary MCMV latent reactivation infection and evaluation. There are few problems such as few means to achieve the effect of novel construction method, long duration and accurate animal model.

Inactive Publication Date: 2020-11-20
张志辉
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Problems solved by technology

However, there are no relevant reports on how to construct an experimental mouse model of latent reactivation infection of MCMV in the lungs, and due to tissue differences and different detection substances, the above existing methods are not suitable for latent reactivation infection of lung MCMV Established, and the above-mentioned existing methods have too short construction time and few evaluation methods, and cannot comprehensively evaluate the impact of HCMV on the body tissue

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  • Method for establishing mouse lung cytomegalovirus latent reactivation infection model
  • Method for establishing mouse lung cytomegalovirus latent reactivation infection model
  • Method for establishing mouse lung cytomegalovirus latent reactivation infection model

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Embodiment 1

[0039] Example 1 Establishment and evaluation of latent reactivation infection model of cytomegalovirus in mouse lung

[0040] 1. Establish the latent infection of cytomegalovirus in the lungs of experimental mice

[0041] (1) Animal selection and handling: 6- to 8-week-old female BALB / c mice were used. After obtaining the approval of animal ethics, the mice were kept in a level 2 animal biosafety laboratory. Mice were euthanized using the cervical dislocation method after inhalation of the anesthetic isoflurane. The mouse lungs and salivary glands were aseptically dissected, and the removed lungs and salivary glands were immediately frozen in liquid nitrogen and then stored in a -80°C freezer.

[0042] (2) Construction of latent MCMV-infected mice: the purified MCMV Smith virus strain (VR-194 / 1981) was obtained from the American Type Culture Collection. By intraperitoneal injection of 5 × 10 4 The PFU MCMV Smith virus strain achieved primary MCMV infection. After MCMV in...

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Abstract

The invention discloses a method for establishing a mouse lung cytomegalovirus latent reactivation infection model. The method comprises the following steps: injecting MCMV into the abdominal cavity of a mouse to establish mouse lung latent infection cytomegalovirus, selecting latent MCMV-infected mouse, orally taking a medicine prednisone to induce reactivation of the lung latent cytomegalovirus,and identifying reactivation of the lung latent cytomegalovirus. Lung latent cytomegalovirus infection is induced to be reactivated by orally taking the medicine prednisone, the mouse lung is subjected to cytomegalovirus latent reactivation infection, and an animal model with cytomegalovirus latent reactivation can be constructed. The mouse model shows the typical characteristic of lung cytomegalovirus latent reactivation that: a virus expression-related protein appears in lung tissues. The model can be used for fundamental research in the related fields of cytomegalovirus infection, and laysan experimental animal foundation for exploring the pathogenesis of latent reactivation of cytomegalovirus.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for establishing a latent reactivation infection model of mouse pulmonary cytomegalovirus. Background technique [0002] Human cytomegalovirus (Human Cytomegalovirus, HCMV) has an extremely high infection rate in the population, and its infection rate is related to factors such as gender, race, age group, region, and economic status. The overall population infection rate is about 60-100%. HCMV in newborns in my country is as high as 96.2%. HCMV has the characteristics of herpes virus infection, that is, once the human body is infected with HCMV, it will be carried for life, and can lead to adverse outcomes such as congenital nervous system dysplasia, induction of transplanted organ rejection, and weakened immune defenses, which seriously threaten life and health. However, current treatment and preventive measures not much. [0003] Cytomegalovirus (CMV) has obvio...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A61K31/573
CPCA01K67/02A01K2207/20A01K2227/105A01K2267/0337A61K31/573
Inventor 张志辉
Owner 张志辉
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