Periodontitis diagnosis method, application and kit

Abstract

An in vitro method for assessing whether a human patient has periodontitis is disclosed. The method is based on determining insights to biomarker proteins. Thus, in a saliva sample from a patient suffering from periodontitis, concentrations of two or more proteins of matrix metalloproteinase 8 (MMP8), matrix metalloproteinase 9 (MMP9), pyruvate kinase (PK), and S100 calcium binding protein A8 (S100A8) are measured. Based on the measured concentration, a value reflecting the combined concentration of the protein is determined. The values are compared to a threshold that reflects the combined concentration associated with periodontitis in the same manner. This comparison allows evaluating whether the test value indicates that the patient has periodontitis. Thus, generally, the combined concentration reflected by the test value is below the combined concentration reflected by the threshold value indicating that the patient does not suffer from periodontitis, and the combined concentrationreflected by the test value is equal to or above the combined concentration reflected by the threshold value indicating that the patient suffers from periodontitis.

Description

technical field [0001] The present invention belongs to the field of oral care and relates to saliva-based diagnosis of periodontal disease. In particular, the present invention relates to a kit, use and method for diagnosing periodontitis. Background technique [0002] Inflammation of the gums or gingivitis is a non-destructive periodontal disease mainly caused by the adhesion of dental bacterial biofilm or plaque to the tooth surface. If undetected and treated, reversible gingivitis typically triggers inflammation of the tissue surrounding the tooth (the periodontal tissue), a condition defined as periodontitis, which is irreversible and leads to tissue destruction and Loss of alveolar bone, eventually leading to tooth loss. As gum disease develops, there are often clinical signs and symptoms associated with it, such as gum swelling, changing color from pink to dark red, bleeding gums, bad breath, and gums that become tender or painful to the touch. [0003] Periodontit...

AI Technical Summary

Problems solved by technology

[0008] Factors that have previously limited the development of point-of-care tests for saliva biomarkers include a lack of technology suitable for chairside applications and the inability to analyze multiple biomarkers in individual samples

Moreover, the choice of which multiple biomarkers to include in such tests has not been adequately addressed in the literature, nor has it been implemented in actual testing

Images