Periodontitis diagnostic methods, uses and kits

Abstract

Disclosed is an in vitro method for assessing whether a human patient has periodontitis. The method is based on the insight to determine biomarker proteins. Accordingly, in a sample of saliva a patient suffering from periodontitis, the concentrations are measured of the certain protein combinations. One such combination is Alpha-1-acid glycoprotein (A1AGP) and at least one of Profilin and S100 calcium-binding protein A9 (S100A9). Another combination is Pyruvate Kinase (PK) and S100 calcium-binding protein A8 (S100A8). Based on the concentrations as measured, a value is determined reflecting the joint concentrations for said proteins. This value is compared with a threshold value reflecting in the same manner the joint concentrations associated with periodontitis. The comparison allows assessing whether the testing value is indicative of the presence of periodontitis in said patient. Thereby, typically, a testing value reflecting a joint concentration below the joint concentration reflected by the threshold value is indicative for absence of periodontitis in said patient, and a testing value reflecting a joint concentration at or above the joint concentration reflected by the threshold value, is indicative for periodontitis in said patient.

Description

technical field [0001] The present invention belongs to the field of oral care and relates to saliva-based diagnosis of periodontal disease. In particular, the present invention relates to a kit, use and method for diagnosing periodontitis. Background technique [0002] Inflammation of the gums or gingivitis is a non-destructive periodontal disease mainly caused by the adhesion of dental bacterial biofilm or plaque to the tooth surface. If undetected and treated, reversible gingivitis typically causes inflammation of the tissue surrounding the tooth (the periodontal tissue), a condition defined as periodontitis, which is irreversible and leads to tissue destruction and tooth decay. The groove bone is missing and eventually the tooth is lost. As gum disease develops, there are often clinical signs and symptoms associated with it, such as gum swelling, changing color from pink to dark red, bleeding gums, bad breath, and gums that become tender or painful to the touch. [00...

AI Technical Summary

Problems solved by technology

[0008] Factors that have previously limited the development of point-of-care tests for saliva biomarkers include a lack of technology suitable for chairside application and the inability to analyze multiple biomarkers in individual samples

Moreover, the choice of which multiple biomarkers to include in such tests has not been adequately addressed in the literature, nor has it been implemented in actual testing

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